Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC
Several pre-analytical variables influence hematological results, including anticoagulant use, storage temperature, and time between blood sample collection and analysis. Delayed sample analysis owing to prolonged storage could result in erythrogram profiles, which could complicate the interpretatio...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga, Faculty of Veterinary Medicine
2024-04-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/49540 |
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| author | Putri Azzahrah Anita Esfandiari Arief Purwo Mihardi Sus Derthi Widhyari Retno Wulansari Putri Indah Ningtias |
| author_facet | Putri Azzahrah Anita Esfandiari Arief Purwo Mihardi Sus Derthi Widhyari Retno Wulansari Putri Indah Ningtias |
| author_sort | Putri Azzahrah |
| collection | DOAJ |
| description | Several pre-analytical variables influence hematological results, including anticoagulant use, storage temperature, and time between blood sample collection and analysis. Delayed sample analysis owing to prolonged storage could result in erythrogram profiles, which could complicate the interpretation of the resulting data. This study investigated the erythrogram profile of tripotassium ethylenediaminetetraacetic acid (K3EDTA) in blood samples stored for 48 h at 4°C. Ten healthy blood samples of Ongole crossbred cattle were collected into K3EDTA tubes from the jugular or coccygeal veins and analyzed for erythrogram profiles (erythrocyte counts, hemoglobin levels, hematocrit value, and erythrocyte morphology). Blood sample analysis for the control (0 h) was performed within ± 1.5 hours after collection, then the samples were refrigerated (4°C) and analyzed at 3, 6, 9, 12, 24, and 48 h. The results showed increased (p < 0.05) erythrocyte counts and hematocrit values after 9–24 and 6–48 h of storage, respectively. There was a significant difference in erythrocyte diameter between 0 h and other time observations (p < 0.05). Echinocytes were observed at 0 h of storage and continued to increase up to 48 h. Hypochromasia was also found at 6 to 48 hours of storage. Therefore, the analysis of blood samples for erythrogram parameters should be performed as soon as possible, preferably within three hours after collection, to ensure clinically reliable results. |
| format | Article |
| id | doaj-art-c6b7c20cfa58474aaba50cc8d5a47ff4 |
| institution | Kabale University |
| issn | 2615-7497 2581-012X |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj-art-c6b7c20cfa58474aaba50cc8d5a47ff42025-01-02T22:24:00ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2024-04-0171768710.20473/jmv.vol7.iss1.2024.76-8747687Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oCPutri Azzahrah0https://orcid.org/0009-0009-3480-1264Anita Esfandiari1https://orcid.org/0000-0002-1435-6185Arief Purwo Mihardi2https://orcid.org/0000-0001-7044-8204Sus Derthi Widhyari3https://orcid.org/0000-0003-3030-9482Retno Wulansari4https://orcid.org/0000-0002-3420-5931Putri Indah Ningtias5https://orcid.org/0009-0008-8871-1355Veterinary Medicine Study Program, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, West Java, IndonesiaDivision of Veterinary Internal Medicine, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, West Java, IndonesiaDivision of Veterinary Internal Medicine, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, West Java, IndonesiaDivision of Veterinary Internal Medicine, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, West Java, IndonesiaDivision of Veterinary Internal Medicine, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, West Java, IndonesiaAnimal Embryo Center, Cipelang, Bogor, West Java, IndonesiaSeveral pre-analytical variables influence hematological results, including anticoagulant use, storage temperature, and time between blood sample collection and analysis. Delayed sample analysis owing to prolonged storage could result in erythrogram profiles, which could complicate the interpretation of the resulting data. This study investigated the erythrogram profile of tripotassium ethylenediaminetetraacetic acid (K3EDTA) in blood samples stored for 48 h at 4°C. Ten healthy blood samples of Ongole crossbred cattle were collected into K3EDTA tubes from the jugular or coccygeal veins and analyzed for erythrogram profiles (erythrocyte counts, hemoglobin levels, hematocrit value, and erythrocyte morphology). Blood sample analysis for the control (0 h) was performed within ± 1.5 hours after collection, then the samples were refrigerated (4°C) and analyzed at 3, 6, 9, 12, 24, and 48 h. The results showed increased (p < 0.05) erythrocyte counts and hematocrit values after 9–24 and 6–48 h of storage, respectively. There was a significant difference in erythrocyte diameter between 0 h and other time observations (p < 0.05). Echinocytes were observed at 0 h of storage and continued to increase up to 48 h. Hypochromasia was also found at 6 to 48 hours of storage. Therefore, the analysis of blood samples for erythrogram parameters should be performed as soon as possible, preferably within three hours after collection, to ensure clinically reliable results.https://e-journal.unair.ac.id/JMV/article/view/49540erythrogramerythrocyte morphologyk3edta anticoagulantstorage duration |
| spellingShingle | Putri Azzahrah Anita Esfandiari Arief Purwo Mihardi Sus Derthi Widhyari Retno Wulansari Putri Indah Ningtias Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC Jurnal Medik Veteriner erythrogram erythrocyte morphology k3edta anticoagulant storage duration |
| title | Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC |
| title_full | Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC |
| title_fullStr | Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC |
| title_full_unstemmed | Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC |
| title_short | Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC |
| title_sort | erythrogram profile of blood samples anticoagulated with tri potassium ethylene diamine tetraacetic acid k3edta stored for 48 hours at 4oc |
| topic | erythrogram erythrocyte morphology k3edta anticoagulant storage duration |
| url | https://e-journal.unair.ac.id/JMV/article/view/49540 |
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