Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells
The acute phase of Chagas' disease in mice and human is marked by states of immunosuppression, in which Trypanosoma cruzi replicates extensively and releases immunomodulatory molecules that delay parasite-specific responses mediated by effector T cells. This mechanism of evasion allows the para...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/829139 |
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| author | Yadira Morán-Utrera Aracely López-Monteon José Luis Rosales-Encina Enrique Méndez-Bolaina Angel Ramos-Ligonio |
| author_facet | Yadira Morán-Utrera Aracely López-Monteon José Luis Rosales-Encina Enrique Méndez-Bolaina Angel Ramos-Ligonio |
| author_sort | Yadira Morán-Utrera |
| collection | DOAJ |
| description | The acute phase of Chagas' disease in mice and human is marked by states of immunosuppression, in which Trypanosoma cruzi replicates extensively and releases immunomodulatory molecules that delay parasite-specific responses mediated by effector T cells. This mechanism of evasion allows the parasite to spread in the host. Parasite molecules that regulate the host immune response during Chagas’ disease have not been fully identified, particularly proteins of the amastigote stage. In this work, we evaluated the role of the GPI anchored SSP4 protein of T. cruzi as an immunomodulatory molecule in peripheral blood mononuclear cells (PBMCs). rMBP::SSP4 protein was able to stimulate nitric oxide (NO) production. Likewise, rMBP::SSP4 induced the expression of genes and production of molecules involved in the inflammatory process, such as, cytokines, chemokines, and adhesion molecules (CAMs) as determined by RT-PCR and ELISA. These results suggest that the amastigote SSP4 molecule could play a key role in the immunoregulatory and/or immunosuppressive process observed in the acute phase of infection with T. cruzi. |
| format | Article |
| id | doaj-art-c66214cef7a446a2a308e711abf69f75 |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-c66214cef7a446a2a308e711abf69f752025-02-03T05:47:43ZengWileyJournal of Tropical Medicine1687-96861687-96942012-01-01201210.1155/2012/829139829139Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear CellsYadira Morán-Utrera0Aracely López-Monteon1José Luis Rosales-Encina2Enrique Méndez-Bolaina3Angel Ramos-Ligonio4LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Orizaba, VER, MexicoLADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Orizaba, VER, MexicoLaboratorio de Biología Molecular, Departamento de Infectómica y Patogénesis Molecular, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, DF, MexicoCentro de Investigaciones Biomédicas, Universidad Veracruzana, Xalapa, VER, MexicoLADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Orizaba, VER, MexicoThe acute phase of Chagas' disease in mice and human is marked by states of immunosuppression, in which Trypanosoma cruzi replicates extensively and releases immunomodulatory molecules that delay parasite-specific responses mediated by effector T cells. This mechanism of evasion allows the parasite to spread in the host. Parasite molecules that regulate the host immune response during Chagas’ disease have not been fully identified, particularly proteins of the amastigote stage. In this work, we evaluated the role of the GPI anchored SSP4 protein of T. cruzi as an immunomodulatory molecule in peripheral blood mononuclear cells (PBMCs). rMBP::SSP4 protein was able to stimulate nitric oxide (NO) production. Likewise, rMBP::SSP4 induced the expression of genes and production of molecules involved in the inflammatory process, such as, cytokines, chemokines, and adhesion molecules (CAMs) as determined by RT-PCR and ELISA. These results suggest that the amastigote SSP4 molecule could play a key role in the immunoregulatory and/or immunosuppressive process observed in the acute phase of infection with T. cruzi.http://dx.doi.org/10.1155/2012/829139 |
| spellingShingle | Yadira Morán-Utrera Aracely López-Monteon José Luis Rosales-Encina Enrique Méndez-Bolaina Angel Ramos-Ligonio Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells Journal of Tropical Medicine |
| title | Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
| title_full | Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
| title_fullStr | Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
| title_full_unstemmed | Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
| title_short | Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
| title_sort | trypanosoma cruzi ssp4 amastigote protein induces expression of immunoregulatory and immunosuppressive molecules in peripheral blood mononuclear cells |
| url | http://dx.doi.org/10.1155/2012/829139 |
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