Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin
Abstract Carbon dots doped with metals and non-metals have gained much popularity due to the enhancement in their optical and electronic properties. In this study, polyethyleneimine-functionalized transition metal (nickel or copper) doped carbon dots (CD, NiCD and CuCD) were synthesized through hydr...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-85246-1 |
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| author | A. Lakshmi Devi M. Sreelakshmi P. V. Suneesh T. G. Satheesh Babu |
| author_facet | A. Lakshmi Devi M. Sreelakshmi P. V. Suneesh T. G. Satheesh Babu |
| author_sort | A. Lakshmi Devi |
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| description | Abstract Carbon dots doped with metals and non-metals have gained much popularity due to the enhancement in their optical and electronic properties. In this study, polyethyleneimine-functionalized transition metal (nickel or copper) doped carbon dots (CD, NiCD and CuCD) were synthesized through hydrothermal method. The carbon dots exhibited a blue fluorescence at 470 nm when excited at 350 nm. The as-synthesized carbon dots were utilised for the fluorimetric detection of bilirubin in the range 0.5 µM – 280 µM, with CuCD exhibiting the highest sensitivity of 155.38 a.u/log µM in the concentration range 0.5 to 10 µM and 84.01 a.u/ log µM in the concentration range 10 to 280 µM. CuCD also exhibited the lowest limit of detection of 0.0907 µM and the lowest limit of quantification of 0.3023 µM. All the carbon dots showed negligible interference in the presence of biomolecules and metal ions present in human serum implying the remarkable selectivity of the method to bilirubin detection. Further, the carbon dots were successfully tested for their real-time application in human serum using bilirubin-spiked serum samples. |
| format | Article |
| id | doaj-art-c6323592e65a4afdb4450876fd5a801b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-c6323592e65a4afdb4450876fd5a801b2025-01-12T12:23:44ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-025-85246-1Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubinA. Lakshmi Devi0M. Sreelakshmi1P. V. Suneesh2T. G. Satheesh Babu3Department of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa VidyapeethamDepartment of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa VidyapeethamDepartment of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa VidyapeethamDepartment of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa VidyapeethamAbstract Carbon dots doped with metals and non-metals have gained much popularity due to the enhancement in their optical and electronic properties. In this study, polyethyleneimine-functionalized transition metal (nickel or copper) doped carbon dots (CD, NiCD and CuCD) were synthesized through hydrothermal method. The carbon dots exhibited a blue fluorescence at 470 nm when excited at 350 nm. The as-synthesized carbon dots were utilised for the fluorimetric detection of bilirubin in the range 0.5 µM – 280 µM, with CuCD exhibiting the highest sensitivity of 155.38 a.u/log µM in the concentration range 0.5 to 10 µM and 84.01 a.u/ log µM in the concentration range 10 to 280 µM. CuCD also exhibited the lowest limit of detection of 0.0907 µM and the lowest limit of quantification of 0.3023 µM. All the carbon dots showed negligible interference in the presence of biomolecules and metal ions present in human serum implying the remarkable selectivity of the method to bilirubin detection. Further, the carbon dots were successfully tested for their real-time application in human serum using bilirubin-spiked serum samples.https://doi.org/10.1038/s41598-025-85246-1Carbon dotsCopper-doped carbon dotsNickel-doped carbon dotsBilirubinFluorescence quenchingBiosensor |
| spellingShingle | A. Lakshmi Devi M. Sreelakshmi P. V. Suneesh T. G. Satheesh Babu Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin Scientific Reports Carbon dots Copper-doped carbon dots Nickel-doped carbon dots Bilirubin Fluorescence quenching Biosensor |
| title | Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin |
| title_full | Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin |
| title_fullStr | Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin |
| title_full_unstemmed | Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin |
| title_short | Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin |
| title_sort | copper and nickel doped carbon dots for rapid and sensitive fluorescent turn off detection of bilirubin |
| topic | Carbon dots Copper-doped carbon dots Nickel-doped carbon dots Bilirubin Fluorescence quenching Biosensor |
| url | https://doi.org/10.1038/s41598-025-85246-1 |
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