Nature-driven blue-emissive N, S-CDs: Harnessing sequential “switch-off-on” fluorescence signals for detection of chrysin and Al³⁺ along with cellular imaging versatility
Novel biocompatible blue emissive nitrogen and sulfur co-doped carbon dots (N, S-CDs) were synthesized via in situ microwave-assisted synthesis from snake melon (Cucumis melo var. flexuosus) for the first time. The N, S-CDs have been utilized for fluorimetric determination of chrysin (CHR). The fluo...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
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| Series: | Talanta Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831925000682 |
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| Summary: | Novel biocompatible blue emissive nitrogen and sulfur co-doped carbon dots (N, S-CDs) were synthesized via in situ microwave-assisted synthesis from snake melon (Cucumis melo var. flexuosus) for the first time. The N, S-CDs have been utilized for fluorimetric determination of chrysin (CHR). The fluorescence emitted by the N, S-CDs (λem = 460 nm) decreased upon the addition of CHR due to dynamic quenching and the inner filter effect (IFE). The reduction in emission intensity exhibits a linear relationship over a concentration range of 2.0–275.0 μM with a low limit of detection (LOD) of 0.0837 μM. By contrast, the quenched fluorescence of the N, S-CD/CHR system was restored upon the addition of Al³⁺, which efficiently displaced CHR from the surface of the N, S-CDs, prompting fluorescence ''switch-on''. The retrieved fluorescence intensity was proportional to Al3+ concentration over a range of 50.0–4000.0 μM, with a LOD of 2.96 μM. This sequential approach was efficiently utilized to determine CHR and Al3+ in real pharmaceutical, biological, and environmental samples with accurate and precise findings. Furthermore, due to their minimal cytotoxicity, the N, S-CDs were also successful for cellular imaging on HepG2 cells. The proposed sensing platform provides several merits, including simplicity, cost-efficiency, long-lasting stability, high selectivity, and exceptional sensitivity. These findings not only contribute to the growing field of carbon-based nanomaterials but also offer a sustainable and multifunctional platform for environmental and biomedical applications. |
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| ISSN: | 2666-8319 |