Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast.
Gpn2 is a highly conserved protein essential for the assembly of RNA polymerase II (RNAPII) in eukaryotic cells. Mutations in Gpn2, specifically Phe105Tyr and Leu164Pro, confer temperature sensitivity and significantly impair RNAPII assembly. Despite its crucial role, the complete range of Gpn2 func...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0313597 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846129492889698304 |
|---|---|
| author | Le Wang Pan Li Pei Zeng Debao Xie Mengdi Gao Lujie Ma Aamir Sohail Fanli Zeng |
| author_facet | Le Wang Pan Li Pei Zeng Debao Xie Mengdi Gao Lujie Ma Aamir Sohail Fanli Zeng |
| author_sort | Le Wang |
| collection | DOAJ |
| description | Gpn2 is a highly conserved protein essential for the assembly of RNA polymerase II (RNAPII) in eukaryotic cells. Mutations in Gpn2, specifically Phe105Tyr and Leu164Pro, confer temperature sensitivity and significantly impair RNAPII assembly. Despite its crucial role, the complete range of Gpn2 functions remains to be elucidated. To further explore these functions, we conducted large-scale multicopy suppressor screening in budding yeast, aiming to identify genes whose overexpression could mitigate the growth defects of a temperature-sensitive gpn2 mutant (gpn2ts) at restrictive temperatures. We screened over 30,000 colonies harboring plasmids from a multicopy genetic library and identified 31 genes that rescued the growth defects of gpn2ts to various extents. Notably, we found that PAB1, CDC5, and RGS2 reduced the drug sensitivity of gpn2ts mutants. These findings lay a theoretical foundation for future studies on the function of Gpn2 in RNAPII assembly. |
| format | Article |
| id | doaj-art-c55f0ace364e4a8f92816712fbafed30 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c55f0ace364e4a8f92816712fbafed302024-12-10T05:32:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031359710.1371/journal.pone.0313597Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast.Le WangPan LiPei ZengDebao XieMengdi GaoLujie MaAamir SohailFanli ZengGpn2 is a highly conserved protein essential for the assembly of RNA polymerase II (RNAPII) in eukaryotic cells. Mutations in Gpn2, specifically Phe105Tyr and Leu164Pro, confer temperature sensitivity and significantly impair RNAPII assembly. Despite its crucial role, the complete range of Gpn2 functions remains to be elucidated. To further explore these functions, we conducted large-scale multicopy suppressor screening in budding yeast, aiming to identify genes whose overexpression could mitigate the growth defects of a temperature-sensitive gpn2 mutant (gpn2ts) at restrictive temperatures. We screened over 30,000 colonies harboring plasmids from a multicopy genetic library and identified 31 genes that rescued the growth defects of gpn2ts to various extents. Notably, we found that PAB1, CDC5, and RGS2 reduced the drug sensitivity of gpn2ts mutants. These findings lay a theoretical foundation for future studies on the function of Gpn2 in RNAPII assembly.https://doi.org/10.1371/journal.pone.0313597 |
| spellingShingle | Le Wang Pan Li Pei Zeng Debao Xie Mengdi Gao Lujie Ma Aamir Sohail Fanli Zeng Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast. PLoS ONE |
| title | Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast. |
| title_full | Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast. |
| title_fullStr | Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast. |
| title_full_unstemmed | Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast. |
| title_short | Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast. |
| title_sort | dosage suppressors of gpn2ts mutants and functional insights into the role of gpn2 in budding yeast |
| url | https://doi.org/10.1371/journal.pone.0313597 |
| work_keys_str_mv | AT lewang dosagesuppressorsofgpn2tsmutantsandfunctionalinsightsintotheroleofgpn2inbuddingyeast AT panli dosagesuppressorsofgpn2tsmutantsandfunctionalinsightsintotheroleofgpn2inbuddingyeast AT peizeng dosagesuppressorsofgpn2tsmutantsandfunctionalinsightsintotheroleofgpn2inbuddingyeast AT debaoxie dosagesuppressorsofgpn2tsmutantsandfunctionalinsightsintotheroleofgpn2inbuddingyeast AT mengdigao dosagesuppressorsofgpn2tsmutantsandfunctionalinsightsintotheroleofgpn2inbuddingyeast AT lujiema dosagesuppressorsofgpn2tsmutantsandfunctionalinsightsintotheroleofgpn2inbuddingyeast AT aamirsohail dosagesuppressorsofgpn2tsmutantsandfunctionalinsightsintotheroleofgpn2inbuddingyeast AT fanlizeng dosagesuppressorsofgpn2tsmutantsandfunctionalinsightsintotheroleofgpn2inbuddingyeast |