Characteristics of the hemagglutinins antibody responses in influenza A patients in Suez Governorate, Egypt

Background: Influenza is an acute respiratory illness caused by the Influenza virus. There are 3 types of Influenza virus A, B and C. Infection with the Influenza virus exhibits broad immune responses and protection from re-infection by the same type of virus. The protective humoral immune response...

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Main Authors: Rehab Tash, Ahmed Mohamed, Said Hamed Abbadi, Mai Rashad
Format: Article
Language:English
Published: Zagazig University, Faculty of Medicine 2024-11-01
Series:Microbes and Infectious Diseases
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Online Access:https://mid.journals.ekb.eg/article_379967_00a24dd1afe8f969071e64706cfe0f90.pdf
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author Rehab Tash
Ahmed Mohamed
Said Hamed Abbadi
Mai Rashad
author_facet Rehab Tash
Ahmed Mohamed
Said Hamed Abbadi
Mai Rashad
author_sort Rehab Tash
collection DOAJ
description Background: Influenza is an acute respiratory illness caused by the Influenza virus. There are 3 types of Influenza virus A, B and C. Infection with the Influenza virus exhibits broad immune responses and protection from re-infection by the same type of virus. The protective humoral immune response depends mainly on antibodies to the virus surface glycoprotein, hemagglutinin, and neuraminidase. Our study aims to reveal the characteristics of hemagglutinin reactive serum antibody responses in influenza patients in Suez Governorate. Methods: 160 cases were enrolled according to their clinical picture and symptoms onset not more than 10 days from the beginning of illness. Nasopharyngeal swabs were obtained from each case and tested for the influenza virus by rapid influenza antigen detection test. The proven influenza A samples were typed by real-time PCR. Each sample was mapped for their hemagglutinin (HA) antibodies rising titer by ELISA: group 1 HAs (H1, H5, H9) and group 2 HAs (H3 and H7). Results: 15 cases were diagnosed as influenza A with subtype H1N1. There was a rise in group 1HAs more than group 2 HAs when comparing the antibody titer of the 1st and 2nd serum samples. Group 1: H1 showed a 20-fold rise, H5 showed a 5-fold rise and H9 showed a 2-fold rise. Group 2: H3 and H9 showed a 5-fold rise. Conclusion: Following natural infection; Influenza A(H1N1) pdm09 virus induces a strong anti-HA immune response.
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spelling doaj-art-c52765576b7f4c8d9c73c115dc2ed7902024-12-21T21:03:54ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-11-01541297130610.21608/mid.2024.311201.2147379967Characteristics of the hemagglutinins antibody responses in influenza A patients in Suez Governorate, EgyptRehab Tash0Ahmed Mohamed1Said Hamed Abbadi2Mai Rashad3Medical Microbiology and Immunology Department, Faculty pf Medicine, Zagazig University, EgyptMedical Microbiology and Immunology Department, Faculty pf Medicine, Zagazig University, EgyptMedical Microbiology and Immunology Department, Faculty pf Medicine, Suez University, EgyptMedical Microbiology and Immunology Department, Faculty pf Medicine, Suez University, EgyptBackground: Influenza is an acute respiratory illness caused by the Influenza virus. There are 3 types of Influenza virus A, B and C. Infection with the Influenza virus exhibits broad immune responses and protection from re-infection by the same type of virus. The protective humoral immune response depends mainly on antibodies to the virus surface glycoprotein, hemagglutinin, and neuraminidase. Our study aims to reveal the characteristics of hemagglutinin reactive serum antibody responses in influenza patients in Suez Governorate. Methods: 160 cases were enrolled according to their clinical picture and symptoms onset not more than 10 days from the beginning of illness. Nasopharyngeal swabs were obtained from each case and tested for the influenza virus by rapid influenza antigen detection test. The proven influenza A samples were typed by real-time PCR. Each sample was mapped for their hemagglutinin (HA) antibodies rising titer by ELISA: group 1 HAs (H1, H5, H9) and group 2 HAs (H3 and H7). Results: 15 cases were diagnosed as influenza A with subtype H1N1. There was a rise in group 1HAs more than group 2 HAs when comparing the antibody titer of the 1st and 2nd serum samples. Group 1: H1 showed a 20-fold rise, H5 showed a 5-fold rise and H9 showed a 2-fold rise. Group 2: H3 and H9 showed a 5-fold rise. Conclusion: Following natural infection; Influenza A(H1N1) pdm09 virus induces a strong anti-HA immune response.https://mid.journals.ekb.eg/article_379967_00a24dd1afe8f969071e64706cfe0f90.pdfkeywords: influenzaantigenhaemagglutininantibodiesreal time pcr
spellingShingle Rehab Tash
Ahmed Mohamed
Said Hamed Abbadi
Mai Rashad
Characteristics of the hemagglutinins antibody responses in influenza A patients in Suez Governorate, Egypt
Microbes and Infectious Diseases
keywords: influenza
antigen
haemagglutinin
antibodies
real time pcr
title Characteristics of the hemagglutinins antibody responses in influenza A patients in Suez Governorate, Egypt
title_full Characteristics of the hemagglutinins antibody responses in influenza A patients in Suez Governorate, Egypt
title_fullStr Characteristics of the hemagglutinins antibody responses in influenza A patients in Suez Governorate, Egypt
title_full_unstemmed Characteristics of the hemagglutinins antibody responses in influenza A patients in Suez Governorate, Egypt
title_short Characteristics of the hemagglutinins antibody responses in influenza A patients in Suez Governorate, Egypt
title_sort characteristics of the hemagglutinins antibody responses in influenza a patients in suez governorate egypt
topic keywords: influenza
antigen
haemagglutinin
antibodies
real time pcr
url https://mid.journals.ekb.eg/article_379967_00a24dd1afe8f969071e64706cfe0f90.pdf
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