Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin
<b>Aim: </b>The purpose was to investigate the effects of various dosages of thymoquinone and resveratrol (5 and 20 mg/kg) on doxorubicin-induced hepatotoxicity in rats from a pathological standpoint.<p> <b>Materials and Methods:</b> Eighty male Wistar Albino rats were...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2022-09-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1398 |
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| Summary: | <b>Aim: </b>The purpose was to investigate the effects of various dosages of
thymoquinone and resveratrol (5 and 20 mg/kg) on doxorubicin-induced
hepatotoxicity in rats from a pathological standpoint.<p>
<b>Materials and Methods:</b> Eighty male Wistar Albino rats were used in
this study. Animals were divided in to 10 groups: Control (physiological
saline, PO); Doxorubicin (physiological saline, PO and Dox,15mg/kg Dox in
10th days, IP); Thymoquinone -5 (TQ-5, 5 mg/kg TQ, PO); TQ-20 (20 mg/
kg TQ, PO); Resveratrol-5 (Res-5, 5 mg/kg Res, PO); Res-20 (20 mg/kg Res,
PO); Dox+TQ-5 ; Dox+TQ-20; Dox+Res-5; Dox+Res-20. After the 21-day
experiment, 6 replicates were randomly selected from the groups. After
weighing the body weight, the livers of the euthanized rats were dissected
and weighed. Routine tissue processing processes were applied to liver
samples. Hepatocyte degeneration, necrosis/apoptosis, bile duct hyperplasia,
dissociation, congestion, karyomegaly, mononuclear cell infiltration, binuclear
hepatocytes, and mitosis were all examined microscopically, and a liver total
lesion score was calculated<p>
<b>Results:</b> Dox treatment increased relative liver weight, but the TQ and Res
groups prevented this increase (p<0.05). The liver total lesion score, which
increased with Dox, was shown to be lower in the TQ and Res groups (p<0.05).
However, the Dox+TQ-5, Dox+TQ-20, and Dox+Res-20 groups, showed no
amelioration in necrosis/apoptosis.<p>
<b>Conclusion: </b>TQ and Res (5 and 20 mg/kg) decreased the total liver lesion
score induced by Dox. Although TQ and RES diminish degeneration and
inflammation, their poor protective effects on necrosis/apoptosis, one of the
key criteria, were considered as a limiting cause for their uncontrolled usage. |
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| ISSN: | 1309-6958 2146-1953 |