Inducing Oxalobacter formigenes Colonization Reduces Urinary Oxalate in Healthy Adults
Introduction: Oxalate-degrading intestinal bacteria, including the oxalate-degrading specialist, Oxalobacter formigenes (O formigenes), have the potential to reduce urinary oxalate excretion in humans, and thus limit the risk of calcium oxalate kidney stone formation. The aim of this proof-of-concep...
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Elsevier
2025-05-01
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| Series: | Kidney International Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468024925000932 |
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| author | Sonia Fargue Mangesh Suryavanshi Kyle D. Wood Joseph J. Crivelli Robert A. Oster Dean G. Assimos Aaron Miller John Knight |
| author_facet | Sonia Fargue Mangesh Suryavanshi Kyle D. Wood Joseph J. Crivelli Robert A. Oster Dean G. Assimos Aaron Miller John Knight |
| author_sort | Sonia Fargue |
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| description | Introduction: Oxalate-degrading intestinal bacteria, including the oxalate-degrading specialist, Oxalobacter formigenes (O formigenes), have the potential to reduce urinary oxalate excretion in humans, and thus limit the risk of calcium oxalate kidney stone formation. The aim of this proof-of-concept study, which was performed in healthy adults, was to demonstrate that ingestion of live O formigenes is safe, can establish sustainable gut colonization, and reduce urinary oxalate excretion. Methods: Twenty-two healthy adults without a history of kidney stones and not colonized with O formigenes ingested diets controlled in oxalate and calcium. In these participants, 24-hour urine and stool oxalate levels were quantified using ion chromatography coupled with mass spectrometry before and after ingestion of O formigenes. Results: All 22 participants were successfully colonized after a single dose of O formigenes (∼1010 cells); 10 remained colonized for at least 1 year. Colonization was lost in 11 participants, of whom 9 reported antibiotic use. Six participants who lost colonization were redosed, and 5 were successfully recolonized. Stool oxalate concentration and urine oxalate excretion significantly decreased by 54% and 14%, respectively, with varied responses after colonization. Microbiome molecular analyses of precolonized stool samples highlighted the abundance and diversity of other potential oxalate-degrading bacteria, which may have influenced the effect of O formigenes colonization on urinary oxalate excretion. Conclusion: These findings support future investigations to examine the effectiveness of O formigenes colonization in reducing urinary oxalate excretion in disease cohorts, including calcium oxalate kidney stone formers with enteric hyperoxaluria. |
| format | Article |
| id | doaj-art-c50d6b051ac1440ea55a0d1300c2bc96 |
| institution | Kabale University |
| issn | 2468-0249 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Kidney International Reports |
| spelling | doaj-art-c50d6b051ac1440ea55a0d1300c2bc962025-08-20T03:47:10ZengElsevierKidney International Reports2468-02492025-05-011051518152810.1016/j.ekir.2025.02.004Inducing Oxalobacter formigenes Colonization Reduces Urinary Oxalate in Healthy AdultsSonia Fargue0Mangesh Suryavanshi1Kyle D. Wood2Joseph J. Crivelli3Robert A. Oster4Dean G. Assimos5Aaron Miller6John Knight7Department of Urology, University of Alabama at Birmingham, Birmingham, Alabama, USA; Correspondence: Sonia Fargue, Department of Urology, University of Alabama at Birmingham Heersink School of Medicine, 720 20th St South Kaul 816F, Birmingham, Alabama, USA.Cardiovascular and Metabolic Sciences Department, Cleveland Clinic, Cleveland, Ohio, USADepartment of Urology, University of Alabama at Birmingham, Birmingham, Alabama, USADepartment of Urology, University of Alabama at Birmingham, Birmingham, Alabama, USADivision of Preventive Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USADepartment of Urology, University of Alabama at Birmingham, Birmingham, Alabama, USACardiovascular and Metabolic Sciences Department, Cleveland Clinic, Cleveland, Ohio, USA; Department of Urology, Cleveland Clinic, Cleveland, Ohio, USADepartment of Urology, University of Alabama at Birmingham, Birmingham, Alabama, USAIntroduction: Oxalate-degrading intestinal bacteria, including the oxalate-degrading specialist, Oxalobacter formigenes (O formigenes), have the potential to reduce urinary oxalate excretion in humans, and thus limit the risk of calcium oxalate kidney stone formation. The aim of this proof-of-concept study, which was performed in healthy adults, was to demonstrate that ingestion of live O formigenes is safe, can establish sustainable gut colonization, and reduce urinary oxalate excretion. Methods: Twenty-two healthy adults without a history of kidney stones and not colonized with O formigenes ingested diets controlled in oxalate and calcium. In these participants, 24-hour urine and stool oxalate levels were quantified using ion chromatography coupled with mass spectrometry before and after ingestion of O formigenes. Results: All 22 participants were successfully colonized after a single dose of O formigenes (∼1010 cells); 10 remained colonized for at least 1 year. Colonization was lost in 11 participants, of whom 9 reported antibiotic use. Six participants who lost colonization were redosed, and 5 were successfully recolonized. Stool oxalate concentration and urine oxalate excretion significantly decreased by 54% and 14%, respectively, with varied responses after colonization. Microbiome molecular analyses of precolonized stool samples highlighted the abundance and diversity of other potential oxalate-degrading bacteria, which may have influenced the effect of O formigenes colonization on urinary oxalate excretion. Conclusion: These findings support future investigations to examine the effectiveness of O formigenes colonization in reducing urinary oxalate excretion in disease cohorts, including calcium oxalate kidney stone formers with enteric hyperoxaluria.http://www.sciencedirect.com/science/article/pii/S2468024925000932calcium oxalatehyperoxaluriakidney calculiOxalobacter formigenesprobiotics |
| spellingShingle | Sonia Fargue Mangesh Suryavanshi Kyle D. Wood Joseph J. Crivelli Robert A. Oster Dean G. Assimos Aaron Miller John Knight Inducing Oxalobacter formigenes Colonization Reduces Urinary Oxalate in Healthy Adults Kidney International Reports calcium oxalate hyperoxaluria kidney calculi Oxalobacter formigenes probiotics |
| title | Inducing Oxalobacter formigenes Colonization Reduces Urinary Oxalate in Healthy Adults |
| title_full | Inducing Oxalobacter formigenes Colonization Reduces Urinary Oxalate in Healthy Adults |
| title_fullStr | Inducing Oxalobacter formigenes Colonization Reduces Urinary Oxalate in Healthy Adults |
| title_full_unstemmed | Inducing Oxalobacter formigenes Colonization Reduces Urinary Oxalate in Healthy Adults |
| title_short | Inducing Oxalobacter formigenes Colonization Reduces Urinary Oxalate in Healthy Adults |
| title_sort | inducing oxalobacter formigenes colonization reduces urinary oxalate in healthy adults |
| topic | calcium oxalate hyperoxaluria kidney calculi Oxalobacter formigenes probiotics |
| url | http://www.sciencedirect.com/science/article/pii/S2468024925000932 |
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