Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish
In the field of fish genetic breeding, accurately determining the DNA content and ploidy of fish is of great significance. This article introduces the use of flow cytometry (FCM) to measure the DNA content and conduct ploidy analysis by sampling different tissues of freshwater fish species. It descr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-05-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/10/5/227 |
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| author | Xinyan Zhu Yang Chen Xiaodie Zhang Jiaxu Qiang Lingtao Nie Xinyue Luo Binchao Liang Kuo Chen Fuzhong Yang Rurong Zhao Chun Zhang |
| author_facet | Xinyan Zhu Yang Chen Xiaodie Zhang Jiaxu Qiang Lingtao Nie Xinyue Luo Binchao Liang Kuo Chen Fuzhong Yang Rurong Zhao Chun Zhang |
| author_sort | Xinyan Zhu |
| collection | DOAJ |
| description | In the field of fish genetic breeding, accurately determining the DNA content and ploidy of fish is of great significance. This article introduces the use of flow cytometry (FCM) to measure the DNA content and conduct ploidy analysis by sampling different tissues of freshwater fish species. It describes the FCM detection methods and their effectiveness for different individual tissues. These tissues include embryos and fry, as well as the blood, caudal fins, and sperm of adult live fish, and also specific tissues such as testes, ovaries, gills, spleens, and livers under anatomical conditions. Moreover, the application of ploidy detection to different tissues or individuals in different stages in the practice of fish genetic breeding is analyzed. This research covers samples from different growth stages and a variety of tissue types. The results show that this method exhibits high stability and reliability in the detection of different tissue samples, providing solid data support for subsequent research. It holds significant application value in fish genetic breeding. |
| format | Article |
| id | doaj-art-c49e6cb6c1454f3aa3d619c6e263be85 |
| institution | Kabale University |
| issn | 2410-3888 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj-art-c49e6cb6c1454f3aa3d619c6e263be852025-08-20T03:47:57ZengMDPI AGFishes2410-38882025-05-0110522710.3390/fishes10050227Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of FishXinyan Zhu0Yang Chen1Xiaodie Zhang2Jiaxu Qiang3Lingtao Nie4Xinyue Luo5Binchao Liang6Kuo Chen7Fuzhong Yang8Rurong Zhao9Chun Zhang10Engineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaCollege of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde 415000, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaEngineering Reseaech Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaIn the field of fish genetic breeding, accurately determining the DNA content and ploidy of fish is of great significance. This article introduces the use of flow cytometry (FCM) to measure the DNA content and conduct ploidy analysis by sampling different tissues of freshwater fish species. It describes the FCM detection methods and their effectiveness for different individual tissues. These tissues include embryos and fry, as well as the blood, caudal fins, and sperm of adult live fish, and also specific tissues such as testes, ovaries, gills, spleens, and livers under anatomical conditions. Moreover, the application of ploidy detection to different tissues or individuals in different stages in the practice of fish genetic breeding is analyzed. This research covers samples from different growth stages and a variety of tissue types. The results show that this method exhibits high stability and reliability in the detection of different tissue samples, providing solid data support for subsequent research. It holds significant application value in fish genetic breeding.https://www.mdpi.com/2410-3888/10/5/227flow cytometry (FCM)fish genetic breedingploidyDAPI |
| spellingShingle | Xinyan Zhu Yang Chen Xiaodie Zhang Jiaxu Qiang Lingtao Nie Xinyue Luo Binchao Liang Kuo Chen Fuzhong Yang Rurong Zhao Chun Zhang Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish Fishes flow cytometry (FCM) fish genetic breeding ploidy DAPI |
| title | Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish |
| title_full | Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish |
| title_fullStr | Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish |
| title_full_unstemmed | Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish |
| title_short | Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish |
| title_sort | application of flow cytometry to determine cell dna content in the genetic breeding of fish |
| topic | flow cytometry (FCM) fish genetic breeding ploidy DAPI |
| url | https://www.mdpi.com/2410-3888/10/5/227 |
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