Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment

Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment

Summary: Pancreatic ductal adenocarcinoma (PDAC) organoids that simulate the tumor microenvironment (TME) are an effective tool to identify how TME affects PDAC malignancy. We present a protocol for generating a fused pancreatic cancer organoid (FPCO) that partly reproduces the TME, including hetero...

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Bibliographic Details
Main Authors: Kenta Takeuchi, Shunsuke Tabe, Yuya Yamamoto, Kenta Takahashi, Megumi Matsuo, Yasuharu Ueno, Masayuki Ohtsuka, Soichiro Morinaga, Yohei Miyagi, Tomoyuki Yamaguchi, Naoki Tanimizu, Hideki Taniguchi
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Cancer
Stem Cells
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007044
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