Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
Summary: Pancreatic ductal adenocarcinoma (PDAC) organoids that simulate the tumor microenvironment (TME) are an effective tool to identify how TME affects PDAC malignancy. We present a protocol for generating a fused pancreatic cancer organoid (FPCO) that partly reproduces the TME, including hetero...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007044 |
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| author | Kenta Takeuchi Shunsuke Tabe Yuya Yamamoto Kenta Takahashi Megumi Matsuo Yasuharu Ueno Masayuki Ohtsuka Soichiro Morinaga Yohei Miyagi Tomoyuki Yamaguchi Naoki Tanimizu Hideki Taniguchi |
| author_facet | Kenta Takeuchi Shunsuke Tabe Yuya Yamamoto Kenta Takahashi Megumi Matsuo Yasuharu Ueno Masayuki Ohtsuka Soichiro Morinaga Yohei Miyagi Tomoyuki Yamaguchi Naoki Tanimizu Hideki Taniguchi |
| author_sort | Kenta Takeuchi |
| collection | DOAJ |
| description | Summary: Pancreatic ductal adenocarcinoma (PDAC) organoids that simulate the tumor microenvironment (TME) are an effective tool to identify how TME affects PDAC malignancy. We present a protocol for generating a fused pancreatic cancer organoid (FPCO) that partly reproduces the TME, including heterogeneous cancer-associated fibroblasts (CAFs), using patient-derived PDAC cells and human-induced pluripotent cell-derived endothelial and mesenchymal cells. We also describe the procedure for analyzing FPCO characteristics. FPCO can provide a platform for establishing a reliable drug screening system.For complete details on the use and execution of this protocol, please refer to Takeuchi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-c48f85f8440a4bc2aa30bc78f63517d4 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-c48f85f8440a4bc2aa30bc78f63517d42025-01-08T04:53:31ZengElsevierSTAR Protocols2666-16672025-03-0161103539Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironmentKenta Takeuchi0Shunsuke Tabe1Yuya Yamamoto2Kenta Takahashi3Megumi Matsuo4Yasuharu Ueno5Masayuki Ohtsuka6Soichiro Morinaga7Yohei Miyagi8Tomoyuki Yamaguchi9Naoki Tanimizu10Hideki Taniguchi11Division of Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-0071, JapanDivision of Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-0071, Japan; Department of General Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8677, JapanDivision of Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-0071, Japan; Department of General Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8677, JapanDivision of Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-0071, Japan; Graduate School of Frontier Sciences, Computational Biology and Medical Science, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8563, JapanDepartment of Regenerative Medicine, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, JapanDivision of Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-0071, JapanDepartment of General Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8677, JapanDepartment of Gastrointestinal Surgery, Kanagawa Cancer Center, 2-3-2 Nakao, Asahi-ku, Yokohama, Kanagawa 241-8515, JapanMolecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, 2-3-2 Nakao, Asahi-ku, Yokohama, Kanagawa 241-8515, JapanSchool of Life Science, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, JapanDivision of Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-0071, Japan; Corresponding authorDivision of Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-0071, Japan; Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan; Department of Gastrointestinal Surgery, Kanagawa Cancer Center, 2-3-2 Nakao, Asahi-ku, Yokohama, Kanagawa 241-8515, JapanSummary: Pancreatic ductal adenocarcinoma (PDAC) organoids that simulate the tumor microenvironment (TME) are an effective tool to identify how TME affects PDAC malignancy. We present a protocol for generating a fused pancreatic cancer organoid (FPCO) that partly reproduces the TME, including heterogeneous cancer-associated fibroblasts (CAFs), using patient-derived PDAC cells and human-induced pluripotent cell-derived endothelial and mesenchymal cells. We also describe the procedure for analyzing FPCO characteristics. FPCO can provide a platform for establishing a reliable drug screening system.For complete details on the use and execution of this protocol, please refer to Takeuchi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007044CancerStem CellsOrganoids |
| spellingShingle | Kenta Takeuchi Shunsuke Tabe Yuya Yamamoto Kenta Takahashi Megumi Matsuo Yasuharu Ueno Masayuki Ohtsuka Soichiro Morinaga Yohei Miyagi Tomoyuki Yamaguchi Naoki Tanimizu Hideki Taniguchi Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment STAR Protocols Cancer Stem Cells Organoids |
| title | Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment |
| title_full | Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment |
| title_fullStr | Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment |
| title_full_unstemmed | Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment |
| title_short | Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment |
| title_sort | protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment |
| topic | Cancer Stem Cells Organoids |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007044 |
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