Polymeric membrane electrode-based biosensor for detection of methadone: Enhancing doping control in athletes
This work aimed to develop a simple, very stable, and sensitive biosensor to detect methadone for athlete doping monitoring by integrating polyaniline and graphitic carbon nitride on a glassy carbon electrode (PANI/g-C3N4/GCE). Using modified g-C3N4/GCE, the deposition approach was used to electroox...
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Elsevier
2024-12-01
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| Series: | Alexandria Engineering Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110016824010421 |
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| _version_ | 1846160425123577856 |
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| author | Guangyu Han Wenjuan Zhang |
| author_facet | Guangyu Han Wenjuan Zhang |
| author_sort | Guangyu Han |
| collection | DOAJ |
| description | This work aimed to develop a simple, very stable, and sensitive biosensor to detect methadone for athlete doping monitoring by integrating polyaniline and graphitic carbon nitride on a glassy carbon electrode (PANI/g-C3N4/GCE). Using modified g-C3N4/GCE, the deposition approach was used to electrooxidize and polymerize aniline. The electrodeposited and polymerized aniline particles on g-C3N4 surfaces were confirmed by the structural analysis results. The addition of g-C3N4 to PANI produced a network film that improved stability and sensitivity and significantly increased electron mobility, according to cyclic-voltammetry and differential-pulse-voltammetry measurements. The DPV responses of PANI/g-C3N4/GCE indicated that this methadone sensor has sensitivity of 0.0891 μA/μM, linear-range of 1–520 μM, and detection limit of 4 nM. In this study, the proposed sensor's methadone detection capabilities were tested using prepared urine samples from four young athletes, ages 26–32. The sensor's accuracy and dependability are demonstrated by the significant recovery values (more than 97.00 %), suitable precision (RSD less than 4.69 %), and agreement between measurements made using the DPV and the methadone test kit. These findings demonstrate the developed sensor's potential to improve athletes' doping control by offering a dependable methadone detection technique. |
| format | Article |
| id | doaj-art-c4894f3ec2b34663a8e5f9777bfa631f |
| institution | Kabale University |
| issn | 1110-0168 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Alexandria Engineering Journal |
| spelling | doaj-art-c4894f3ec2b34663a8e5f9777bfa631f2024-11-22T07:36:23ZengElsevierAlexandria Engineering Journal1110-01682024-12-01108654661Polymeric membrane electrode-based biosensor for detection of methadone: Enhancing doping control in athletesGuangyu Han0Wenjuan Zhang1Physical Education Institute, Xinxiang Medical University, Xinxiang, Henan 453003, ChinaSports Mechanics Laboratory, Jeju University, Jeju Island 63243, South Korea; Corresponding author.This work aimed to develop a simple, very stable, and sensitive biosensor to detect methadone for athlete doping monitoring by integrating polyaniline and graphitic carbon nitride on a glassy carbon electrode (PANI/g-C3N4/GCE). Using modified g-C3N4/GCE, the deposition approach was used to electrooxidize and polymerize aniline. The electrodeposited and polymerized aniline particles on g-C3N4 surfaces were confirmed by the structural analysis results. The addition of g-C3N4 to PANI produced a network film that improved stability and sensitivity and significantly increased electron mobility, according to cyclic-voltammetry and differential-pulse-voltammetry measurements. The DPV responses of PANI/g-C3N4/GCE indicated that this methadone sensor has sensitivity of 0.0891 μA/μM, linear-range of 1–520 μM, and detection limit of 4 nM. In this study, the proposed sensor's methadone detection capabilities were tested using prepared urine samples from four young athletes, ages 26–32. The sensor's accuracy and dependability are demonstrated by the significant recovery values (more than 97.00 %), suitable precision (RSD less than 4.69 %), and agreement between measurements made using the DPV and the methadone test kit. These findings demonstrate the developed sensor's potential to improve athletes' doping control by offering a dependable methadone detection technique.http://www.sciencedirect.com/science/article/pii/S1110016824010421MethadoneG-C3N4PolyanilineUrine sampleElectrochemical sensor |
| spellingShingle | Guangyu Han Wenjuan Zhang Polymeric membrane electrode-based biosensor for detection of methadone: Enhancing doping control in athletes Alexandria Engineering Journal Methadone G-C3N4 Polyaniline Urine sample Electrochemical sensor |
| title | Polymeric membrane electrode-based biosensor for detection of methadone: Enhancing doping control in athletes |
| title_full | Polymeric membrane electrode-based biosensor for detection of methadone: Enhancing doping control in athletes |
| title_fullStr | Polymeric membrane electrode-based biosensor for detection of methadone: Enhancing doping control in athletes |
| title_full_unstemmed | Polymeric membrane electrode-based biosensor for detection of methadone: Enhancing doping control in athletes |
| title_short | Polymeric membrane electrode-based biosensor for detection of methadone: Enhancing doping control in athletes |
| title_sort | polymeric membrane electrode based biosensor for detection of methadone enhancing doping control in athletes |
| topic | Methadone G-C3N4 Polyaniline Urine sample Electrochemical sensor |
| url | http://www.sciencedirect.com/science/article/pii/S1110016824010421 |
| work_keys_str_mv | AT guangyuhan polymericmembraneelectrodebasedbiosensorfordetectionofmethadoneenhancingdopingcontrolinathletes AT wenjuanzhang polymericmembraneelectrodebasedbiosensorfordetectionofmethadoneenhancingdopingcontrolinathletes |