In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticus
Background: Antimicrobial resistance has rendered several anti-infective agents ineffective, necessitating the need to intensify efforts to identify and develop novel drugs against microbial infection. Structural modification of existing antibiotics continues to be one of the areas of intense rese...
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| Format: | Article |
| Language: | English |
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Creative Pharma Assent
2024-10-01
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| Series: | Journal of Applied Pharmaceutical Research |
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| Online Access: | https://japtronline.com/index.php/joapr/article/view/658 |
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| author | Ewura Seidu Yahaya Jesse Azebiik Anak Nana Ama Amissah Collins Obuah Phyllis Elsie Owusu Agyei Roland Osei Saahene Michael Ainooson Anthony Ablordey Martins Ekor |
| author_facet | Ewura Seidu Yahaya Jesse Azebiik Anak Nana Ama Amissah Collins Obuah Phyllis Elsie Owusu Agyei Roland Osei Saahene Michael Ainooson Anthony Ablordey Martins Ekor |
| author_sort | Ewura Seidu Yahaya |
| collection | DOAJ |
| description | Background: Antimicrobial resistance has rendered several anti-infective agents ineffective, necessitating the need to intensify efforts to identify and develop novel drugs against microbial infection. Structural modification of existing antibiotics continues to be one of the areas of intense research focus in recent times. Objective: This study assessed the antibacterial and synergistic activity of fifteen pyrazolyl sulphonamide derivatives in Staphylococcus haemolyticus. Methods: Antibacterial activity was determined using the broth microdilution method. The ability of test compounds to interact with tetracycline was assessed using the checkerboard synergy testing method and the Loewe synergy and antagonism model. Results: Seven compounds (46.6%) were significantly active against the bacteria (MIC 1 µg/mL – 16 µg/mL), and four were confirmed to form synergistic combinations with tetracycline in checkerboard and Loewe analysis. Conclusion: Observations from this study has demonstrated the antibacterial activity and the synergistic potential of the novel pyrazolyl sulphonamides with tetracycline, highlighting the possible role of modified sulphonamides as a rich resource for antimicrobial development. |
| format | Article |
| id | doaj-art-c46891d3c85744f2ad7f791ada72237a |
| institution | Kabale University |
| issn | 2348-0335 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Creative Pharma Assent |
| record_format | Article |
| series | Journal of Applied Pharmaceutical Research |
| spelling | doaj-art-c46891d3c85744f2ad7f791ada72237a2024-11-23T06:01:12ZengCreative Pharma AssentJournal of Applied Pharmaceutical Research2348-03352024-10-0112511612310.69857/joapr.v12i5.658659In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticusEwura Seidu Yahaya0Jesse Azebiik Anak1Nana Ama Amissah2Collins Obuah3Phyllis Elsie Owusu Agyei4https://orcid.org/0009-0002-4010-0594Roland Osei Saahene5Michael Ainooson6Anthony Ablordey7Martins Ekor8Department of Pharmacology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, GhanaDepartment of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, GhanaDepartment of Chemistry, University of Ghana, Legon, GhanaDepartment of Pharmacology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, GhanaDepartment of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, GhanaDepartment of Chemistry, University of Ghana, Legon, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, GhanaDepartment of Pharmacology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, GhanaBackground: Antimicrobial resistance has rendered several anti-infective agents ineffective, necessitating the need to intensify efforts to identify and develop novel drugs against microbial infection. Structural modification of existing antibiotics continues to be one of the areas of intense research focus in recent times. Objective: This study assessed the antibacterial and synergistic activity of fifteen pyrazolyl sulphonamide derivatives in Staphylococcus haemolyticus. Methods: Antibacterial activity was determined using the broth microdilution method. The ability of test compounds to interact with tetracycline was assessed using the checkerboard synergy testing method and the Loewe synergy and antagonism model. Results: Seven compounds (46.6%) were significantly active against the bacteria (MIC 1 µg/mL – 16 µg/mL), and four were confirmed to form synergistic combinations with tetracycline in checkerboard and Loewe analysis. Conclusion: Observations from this study has demonstrated the antibacterial activity and the synergistic potential of the novel pyrazolyl sulphonamides with tetracycline, highlighting the possible role of modified sulphonamides as a rich resource for antimicrobial development.https://japtronline.com/index.php/joapr/article/view/658antibacterial activityantimicrobial resistancesulphonamide derivativessynergistic activitystaphylococcus haemolyticus |
| spellingShingle | Ewura Seidu Yahaya Jesse Azebiik Anak Nana Ama Amissah Collins Obuah Phyllis Elsie Owusu Agyei Roland Osei Saahene Michael Ainooson Anthony Ablordey Martins Ekor In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticus Journal of Applied Pharmaceutical Research antibacterial activity antimicrobial resistance sulphonamide derivatives synergistic activity staphylococcus haemolyticus |
| title | In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticus |
| title_full | In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticus |
| title_fullStr | In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticus |
| title_full_unstemmed | In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticus |
| title_short | In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticus |
| title_sort | in vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against staphylococcus haemolyticus |
| topic | antibacterial activity antimicrobial resistance sulphonamide derivatives synergistic activity staphylococcus haemolyticus |
| url | https://japtronline.com/index.php/joapr/article/view/658 |
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