Eco-Friendly Synthesis of Gold Nanoparticles Using Microcystis elabens for Enhanced Photothermal Therapy Applications
This study presents a strategy for the green biosynthesis of gold nanoparticles (AuNPs) using extracts from Microcystis elabens (ME-AuNPs). The synthesized ME-AuNPs exhibit photothermal responsiveness under 808[Formula: see text]nm near-infrared (NIR) laser irradiation and maintain photostability ev...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2025-01-01
|
| Series: | Materials Open |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S2811086225500025 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849328532174405632 |
|---|---|
| author | Yinchuan Wang Yifan Bo Jiacheng Chen Zhanglong Li Susu Liu Changyuan Yu Jiahui Liu Shihui Wang |
| author_facet | Yinchuan Wang Yifan Bo Jiacheng Chen Zhanglong Li Susu Liu Changyuan Yu Jiahui Liu Shihui Wang |
| author_sort | Yinchuan Wang |
| collection | DOAJ |
| description | This study presents a strategy for the green biosynthesis of gold nanoparticles (AuNPs) using extracts from Microcystis elabens (ME-AuNPs). The synthesized ME-AuNPs exhibit photothermal responsiveness under 808[Formula: see text]nm near-infrared (NIR) laser irradiation and maintain photostability even after four cycles of irradiation. Cellular uptake experiments confirmed the concentration-dependent internalization of ME-AuNPs by 4T1 cells. MTT assays demonstrated good biocompatibility of ME-AuNPs within an appropriate concentration range (0–100[Formula: see text] [Formula: see text]g/mL) in the absence of laser irradiation. Upon laser irradiation, substantial production of reactive oxygen species (ROS) is induced, promoting cell apoptosis and leading to a cell death rate of 91%. Furthermore, western blot and a series of fluorescence assays confirmed that the cytotoxic effect on cancer cells is mediated through the apoptotic pathways involving caspase 3 and Bax/Bcl-2. In conclusion, our synthesized ME-AuNPs possess favorable photothermal conversion characteristics, demonstrating excellent therapeutic efficacy and safety. |
| format | Article |
| id | doaj-art-c45c7c8d48c54fc5aef3c3241fbf095c |
| institution | Kabale University |
| issn | 2811-0862 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Materials Open |
| spelling | doaj-art-c45c7c8d48c54fc5aef3c3241fbf095c2025-08-20T03:47:33ZengWorld Scientific PublishingMaterials Open2811-08622025-01-010310.1142/S2811086225500025Eco-Friendly Synthesis of Gold Nanoparticles Using Microcystis elabens for Enhanced Photothermal Therapy ApplicationsYinchuan Wang0Yifan Bo1Jiacheng Chen2Zhanglong Li3Susu Liu4Changyuan Yu5Jiahui Liu6Shihui Wang7College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaAnalytical Instrumentation Center, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaThis study presents a strategy for the green biosynthesis of gold nanoparticles (AuNPs) using extracts from Microcystis elabens (ME-AuNPs). The synthesized ME-AuNPs exhibit photothermal responsiveness under 808[Formula: see text]nm near-infrared (NIR) laser irradiation and maintain photostability even after four cycles of irradiation. Cellular uptake experiments confirmed the concentration-dependent internalization of ME-AuNPs by 4T1 cells. MTT assays demonstrated good biocompatibility of ME-AuNPs within an appropriate concentration range (0–100[Formula: see text] [Formula: see text]g/mL) in the absence of laser irradiation. Upon laser irradiation, substantial production of reactive oxygen species (ROS) is induced, promoting cell apoptosis and leading to a cell death rate of 91%. Furthermore, western blot and a series of fluorescence assays confirmed that the cytotoxic effect on cancer cells is mediated through the apoptotic pathways involving caspase 3 and Bax/Bcl-2. In conclusion, our synthesized ME-AuNPs possess favorable photothermal conversion characteristics, demonstrating excellent therapeutic efficacy and safety.https://www.worldscientific.com/doi/10.1142/S2811086225500025Microcystis elabensbiosynthesisgold nanoparticlesphotothermal therapy (PTT)anti-cancer |
| spellingShingle | Yinchuan Wang Yifan Bo Jiacheng Chen Zhanglong Li Susu Liu Changyuan Yu Jiahui Liu Shihui Wang Eco-Friendly Synthesis of Gold Nanoparticles Using Microcystis elabens for Enhanced Photothermal Therapy Applications Materials Open Microcystis elabens biosynthesis gold nanoparticles photothermal therapy (PTT) anti-cancer |
| title | Eco-Friendly Synthesis of Gold Nanoparticles Using Microcystis elabens for Enhanced Photothermal Therapy Applications |
| title_full | Eco-Friendly Synthesis of Gold Nanoparticles Using Microcystis elabens for Enhanced Photothermal Therapy Applications |
| title_fullStr | Eco-Friendly Synthesis of Gold Nanoparticles Using Microcystis elabens for Enhanced Photothermal Therapy Applications |
| title_full_unstemmed | Eco-Friendly Synthesis of Gold Nanoparticles Using Microcystis elabens for Enhanced Photothermal Therapy Applications |
| title_short | Eco-Friendly Synthesis of Gold Nanoparticles Using Microcystis elabens for Enhanced Photothermal Therapy Applications |
| title_sort | eco friendly synthesis of gold nanoparticles using microcystis elabens for enhanced photothermal therapy applications |
| topic | Microcystis elabens biosynthesis gold nanoparticles photothermal therapy (PTT) anti-cancer |
| url | https://www.worldscientific.com/doi/10.1142/S2811086225500025 |
| work_keys_str_mv | AT yinchuanwang ecofriendlysynthesisofgoldnanoparticlesusingmicrocystiselabensforenhancedphotothermaltherapyapplications AT yifanbo ecofriendlysynthesisofgoldnanoparticlesusingmicrocystiselabensforenhancedphotothermaltherapyapplications AT jiachengchen ecofriendlysynthesisofgoldnanoparticlesusingmicrocystiselabensforenhancedphotothermaltherapyapplications AT zhanglongli ecofriendlysynthesisofgoldnanoparticlesusingmicrocystiselabensforenhancedphotothermaltherapyapplications AT susuliu ecofriendlysynthesisofgoldnanoparticlesusingmicrocystiselabensforenhancedphotothermaltherapyapplications AT changyuanyu ecofriendlysynthesisofgoldnanoparticlesusingmicrocystiselabensforenhancedphotothermaltherapyapplications AT jiahuiliu ecofriendlysynthesisofgoldnanoparticlesusingmicrocystiselabensforenhancedphotothermaltherapyapplications AT shihuiwang ecofriendlysynthesisofgoldnanoparticlesusingmicrocystiselabensforenhancedphotothermaltherapyapplications |