Electrospinning drug-loaded polycaprolactone/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma
Cutaneous squamous cell carcinoma (cSCC) tumor resection surgery poses challenges due to incomplete cancer cell removal, which increases the risk of local recurrence and micrometastasis, while large-scale surgical wounds are susceptible to severe infections. Therefore, a drug-loaded multi-functional...
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Biomedical Technology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949723X2400031X |
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| author | Yongteng Song Qingxi Hu Suihong Liu Guotai Yao Haiguang Zhang |
| author_facet | Yongteng Song Qingxi Hu Suihong Liu Guotai Yao Haiguang Zhang |
| author_sort | Yongteng Song |
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| description | Cutaneous squamous cell carcinoma (cSCC) tumor resection surgery poses challenges due to incomplete cancer cell removal, which increases the risk of local recurrence and micrometastasis, while large-scale surgical wounds are susceptible to severe infections. Therefore, a drug-loaded multi-functional bilayer nanofibers skin scaffold was fabricated for postoperative wound care of cSCC. Briefly, the antibacterial drug enrofloxacin (ENR) was loaded into polycaprolactone (PCL) nanofibers using electrospinning to form an antibacterial nanofiber membrane (PCL-ENR) as the outer layer of scaffold. The anticancer drug bleomycin (BLM) was loaded into PCL/Gelatin (Gel) nanofibers via electrospinning to form an anticancer nanofiber membrane (PG-BLM) as the inner layer of scaffold. ENR and BLM were successfully loaded into the scaffold. The scaffold had excellent physicochemical properties, with the outer layer exhibiting hydrophobicity and excellent antibacterial activity, and the inner layer showing hydrophilicity and outstanding anticancer activity. The elongation at break and tensile modulus of the scaffold were 26.35 ± 1.61 % and 15.25 ± 1.56 MPa, respectively. In vitro and in vivo experiments suggested that the scaffold not only has good biocompatibility to promote wound healing but also could inhibit the proliferation of A431 cells, which has great potential clinical application in postoperative wound care of cSCC. |
| format | Article |
| id | doaj-art-c44aa88f57c243d799c7bfb9a4faad17 |
| institution | Kabale University |
| issn | 2949-723X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
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| spelling | doaj-art-c44aa88f57c243d799c7bfb9a4faad172024-11-27T05:04:09ZengKeAi Communications Co., Ltd.Biomedical Technology2949-723X2024-12-0186580Electrospinning drug-loaded polycaprolactone/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinomaYongteng Song0Qingxi Hu1Suihong Liu2Guotai Yao3Haiguang Zhang4Rapid Manufacturing Engineering Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, 200444, China; Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai, 200072, ChinaRapid Manufacturing Engineering Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, 200444, China; Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai, 200072, China; National Demonstration Center for Experimental Engineering Training Education, Shanghai University, Shanghai, 200444, ChinaRapid Manufacturing Engineering Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, 200444, China; Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai, 200072, China; Engineering Science and Mechanics Department, Penn State University, University Park, PA, 16802, USADepartment of Dermatology, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China; Corresponding author.Rapid Manufacturing Engineering Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, 200444, China; Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai, 200072, China; National Demonstration Center for Experimental Engineering Training Education, Shanghai University, Shanghai, 200444, China; Corresponding author. Rapid Manufacturing Engineering Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, 200444, China.Cutaneous squamous cell carcinoma (cSCC) tumor resection surgery poses challenges due to incomplete cancer cell removal, which increases the risk of local recurrence and micrometastasis, while large-scale surgical wounds are susceptible to severe infections. Therefore, a drug-loaded multi-functional bilayer nanofibers skin scaffold was fabricated for postoperative wound care of cSCC. Briefly, the antibacterial drug enrofloxacin (ENR) was loaded into polycaprolactone (PCL) nanofibers using electrospinning to form an antibacterial nanofiber membrane (PCL-ENR) as the outer layer of scaffold. The anticancer drug bleomycin (BLM) was loaded into PCL/Gelatin (Gel) nanofibers via electrospinning to form an anticancer nanofiber membrane (PG-BLM) as the inner layer of scaffold. ENR and BLM were successfully loaded into the scaffold. The scaffold had excellent physicochemical properties, with the outer layer exhibiting hydrophobicity and excellent antibacterial activity, and the inner layer showing hydrophilicity and outstanding anticancer activity. The elongation at break and tensile modulus of the scaffold were 26.35 ± 1.61 % and 15.25 ± 1.56 MPa, respectively. In vitro and in vivo experiments suggested that the scaffold not only has good biocompatibility to promote wound healing but also could inhibit the proliferation of A431 cells, which has great potential clinical application in postoperative wound care of cSCC.http://www.sciencedirect.com/science/article/pii/S2949723X2400031XCutaneous squamous cell carcinomaDrug-loadedBilayer nanofibers skin scaffoldElectrospinningPostoperative wound careAntibacterial and anticancer |
| spellingShingle | Yongteng Song Qingxi Hu Suihong Liu Guotai Yao Haiguang Zhang Electrospinning drug-loaded polycaprolactone/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma Biomedical Technology Cutaneous squamous cell carcinoma Drug-loaded Bilayer nanofibers skin scaffold Electrospinning Postoperative wound care Antibacterial and anticancer |
| title | Electrospinning drug-loaded polycaprolactone/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma |
| title_full | Electrospinning drug-loaded polycaprolactone/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma |
| title_fullStr | Electrospinning drug-loaded polycaprolactone/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma |
| title_full_unstemmed | Electrospinning drug-loaded polycaprolactone/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma |
| title_short | Electrospinning drug-loaded polycaprolactone/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma |
| title_sort | electrospinning drug loaded polycaprolactone polycaprolactone gelatin multi functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma |
| topic | Cutaneous squamous cell carcinoma Drug-loaded Bilayer nanofibers skin scaffold Electrospinning Postoperative wound care Antibacterial and anticancer |
| url | http://www.sciencedirect.com/science/article/pii/S2949723X2400031X |
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