Aptamer-dependent full-length cDNA synthesis by overlap extension PCR
Sequencing of the human genome in combination with computational annotation has provided tremendous data on predicted genes. However, for most of them, no corresponding cDNAs are available yet. Furthermore, even where cDNA clones were obtained, gene transcripts often have many different splice varia...
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| Main Authors: | Yasumasa Mitani, Takayuki Nakayama, Matthias Harbers, Yoshihide Hayashizaki |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2004-07-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04371DD02 |
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