Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics
Live-cell imaging generally requires pretreatment with fluorophores to either monitor cellular functions or the dynamics of intracellular processes and structures. We have recently introduced full-field optical coherence tomography for the label-free live-cell imaging of fungi with potential clinica...
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MDPI AG
2024-10-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/10/11/741 |
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| author | Eliott Teston Marc Sautour Léa Boulnois Nicolas Augey Abdellah Dighab Christophe Guillet Dea Garcia-Hermoso Fanny Lanternier Marie-Elisabeth Bougnoux Frédéric Dalle Louise Basmaciyan Mathieu Blot Pierre-Emmanuel Charles Jean-Pierre Quenot Bianca Podac Catherine Neuwirth Claude Boccara Martine Boccara Olivier Thouvenin Thomas Maldiney |
| author_facet | Eliott Teston Marc Sautour Léa Boulnois Nicolas Augey Abdellah Dighab Christophe Guillet Dea Garcia-Hermoso Fanny Lanternier Marie-Elisabeth Bougnoux Frédéric Dalle Louise Basmaciyan Mathieu Blot Pierre-Emmanuel Charles Jean-Pierre Quenot Bianca Podac Catherine Neuwirth Claude Boccara Martine Boccara Olivier Thouvenin Thomas Maldiney |
| author_sort | Eliott Teston |
| collection | DOAJ |
| description | Live-cell imaging generally requires pretreatment with fluorophores to either monitor cellular functions or the dynamics of intracellular processes and structures. We have recently introduced full-field optical coherence tomography for the label-free live-cell imaging of fungi with potential clinical applications for the diagnosis of invasive fungal mold infections. While both the spatial resolution and technical set up of this technology are more likely designed for the histopathological analysis of tissue biopsies, there is to our knowledge no previous work reporting the use of a light interference-based optical technique for direct mycological examination and monitoring of intracellular processes. We describe the first application of dynamic full-field optical transmission tomography (D-FF-OTT) to achieve both high-resolution and live-cell imaging of fungi. First, D-FF-OTT allowed for the precise examination and identification of several elementary structures within a selection of fungal species commonly known to be responsible for invasive fungal infections such as <i>Candida albicans</i>, <i>Aspergillus fumigatus</i>, or <i>Rhizopus arrhizus</i>. Furthermore, D-FF-OTT revealed the intracellular trafficking of organelles and vesicles related to metabolic processes of living fungi, thus opening new perspectives in fast fungal infection diagnostics. |
| format | Article |
| id | doaj-art-c416f1e3bccb47c283ddc6687712cd5a |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-c416f1e3bccb47c283ddc6687712cd5a2024-11-26T18:08:42ZengMDPI AGJournal of Fungi2309-608X2024-10-01101174110.3390/jof10110741Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular DynamicsEliott Teston0Marc Sautour1Léa Boulnois2Nicolas Augey3Abdellah Dighab4Christophe Guillet5Dea Garcia-Hermoso6Fanny Lanternier7Marie-Elisabeth Bougnoux8Frédéric Dalle9Louise Basmaciyan10Mathieu Blot11Pierre-Emmanuel Charles12Jean-Pierre Quenot13Bianca Podac14Catherine Neuwirth15Claude Boccara16Martine Boccara17Olivier Thouvenin18Thomas Maldiney19Lipness Team, Translational Research Center in Molecular Medicine– INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, FranceDepartment of Parasitology/Mycology, Dijon Bourgogne University Hospital, 21000 Dijon, FranceMedical Biology Laboratory, William Morey General Hospital, 71100 Chalon-sur-Saône, FranceLISPEN, Arts et Metiers Institute of Technology, 71100 Chalon-sur-Saône, FranceLISPEN, Arts et Metiers Institute of Technology, 71100 Chalon-sur-Saône, FranceLISPEN, Arts et Metiers Institute of Technology, 71100 Chalon-sur-Saône, FranceTranslational Mycology Research Group, Mycology Department, National Reference Center for Invasive Mycoses and Antifungals, Institut Pasteur, Paris Cité University, 75015 Paris, FranceTranslational Mycology Research Group, Mycology Department, National Reference Center for Invasive Mycoses and Antifungals, Institut Pasteur, Paris Cité University, 75015 Paris, FranceNecker-Enfants Malades Hospital, Assistance Publique-Hôpitaux de Paris, 75015 Paris, FranceDepartment of Parasitology/Mycology, Dijon Bourgogne University Hospital, 21000 Dijon, FranceDepartment of Parasitology/Mycology, Dijon Bourgogne University Hospital, 21000 Dijon, FranceLipness Team, Translational Research Center in Molecular Medicine– INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, FranceLipness Team, Translational Research Center in Molecular Medicine– INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, FranceLipness Team, Translational Research Center in Molecular Medicine– INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, FranceMedical Biology Laboratory, William Morey General Hospital, 71100 Chalon-sur-Saône, FranceDepartment of Bacteriology, University Hospital of Dijon, 21000 Dijon, FranceInstitut Langevin, Ecole Supérieure de Physique et Chimie Industrielle de la ville de Paris, Université PSL, CNRS, 75005 Paris, FranceInstitut de Systématique, Evolution, Biodiversité– (ISYEB-UMR7205), Ecole Normale Supérieure, PSL Research University, 75005 Paris, FranceInstitut Langevin, Ecole Supérieure de Physique et Chimie Industrielle de la ville de Paris, Université PSL, CNRS, 75005 Paris, FranceLipness Team, Translational Research Center in Molecular Medicine– INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, FranceLive-cell imaging generally requires pretreatment with fluorophores to either monitor cellular functions or the dynamics of intracellular processes and structures. We have recently introduced full-field optical coherence tomography for the label-free live-cell imaging of fungi with potential clinical applications for the diagnosis of invasive fungal mold infections. While both the spatial resolution and technical set up of this technology are more likely designed for the histopathological analysis of tissue biopsies, there is to our knowledge no previous work reporting the use of a light interference-based optical technique for direct mycological examination and monitoring of intracellular processes. We describe the first application of dynamic full-field optical transmission tomography (D-FF-OTT) to achieve both high-resolution and live-cell imaging of fungi. First, D-FF-OTT allowed for the precise examination and identification of several elementary structures within a selection of fungal species commonly known to be responsible for invasive fungal infections such as <i>Candida albicans</i>, <i>Aspergillus fumigatus</i>, or <i>Rhizopus arrhizus</i>. Furthermore, D-FF-OTT revealed the intracellular trafficking of organelles and vesicles related to metabolic processes of living fungi, thus opening new perspectives in fast fungal infection diagnostics.https://www.mdpi.com/2309-608X/10/11/741optical tomographymedical mycologyinvasive fungal infectionslive-cell imagingfungal metabolism |
| spellingShingle | Eliott Teston Marc Sautour Léa Boulnois Nicolas Augey Abdellah Dighab Christophe Guillet Dea Garcia-Hermoso Fanny Lanternier Marie-Elisabeth Bougnoux Frédéric Dalle Louise Basmaciyan Mathieu Blot Pierre-Emmanuel Charles Jean-Pierre Quenot Bianca Podac Catherine Neuwirth Claude Boccara Martine Boccara Olivier Thouvenin Thomas Maldiney Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics Journal of Fungi optical tomography medical mycology invasive fungal infections live-cell imaging fungal metabolism |
| title | Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics |
| title_full | Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics |
| title_fullStr | Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics |
| title_full_unstemmed | Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics |
| title_short | Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics |
| title_sort | label free optical transmission tomography for direct mycological examination and monitoring of intracellular dynamics |
| topic | optical tomography medical mycology invasive fungal infections live-cell imaging fungal metabolism |
| url | https://www.mdpi.com/2309-608X/10/11/741 |
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