An AIE fungal vacuole membrane probe toward species differentiation, vacuole formation visualization, and targeted photodynamic therapy
Vacuoles are unique organelles of fungi. The development of probes targeting the vacuoles membrane will enable visualization of physiological processes and precise diagnosis and therapy. Herein, a zwitterionic molecule, MXF-R, comprising of an aggregation-induced emission (AIE) photosensitizing unit...
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Elsevier
2024-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424003909 |
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| author | Bingnan Wang Siyuan Wang Chunyang Li Jianqing Li Meixi Yi Jing-Wen Lyu Bing Gu Ryan T.K. Kwok Jacky W.Y. Lam Anjun Qin Ben Zhong Tang |
| author_facet | Bingnan Wang Siyuan Wang Chunyang Li Jianqing Li Meixi Yi Jing-Wen Lyu Bing Gu Ryan T.K. Kwok Jacky W.Y. Lam Anjun Qin Ben Zhong Tang |
| author_sort | Bingnan Wang |
| collection | DOAJ |
| description | Vacuoles are unique organelles of fungi. The development of probes targeting the vacuoles membrane will enable visualization of physiological processes and precise diagnosis and therapy. Herein, a zwitterionic molecule, MXF-R, comprising of an aggregation-induced emission (AIE) photosensitizing unit and an antibiotic moxifloxacin, was found capable of specifically imaging vacuole membrane and using for targeted antifungal therapy. MXF-R demonstrated a higher signal-to-noise ratio, stronger targeting capability, and better biocompatibility than the commercial probe FM4-64. By using MXF-R, real-time visualization of vacuole formation during Candida albicans (C. albicans) proliferation was achieved. More importantly, owing to its varying staining ability towards different fungus, MXF-R could be used to quickly identify C. albicans in mixed strains by fluorescence imaging. Moreover, MXF-R exhibits a remarkable ability to generate reactive oxygen species under white light, effectively eradicating C. albicans by disrupting membrane structure. This antifungal therapy of membrane damage is more effective than clinical drug fluconazole. Therefore, this work not only presents the initial discovery of a probe targeting vacuolar membrane, but also provides a way to develop novel materials to realize integrated diagnosis and therapy. |
| format | Article |
| id | doaj-art-c411678e1c4a4224bd44aa3334b29af7 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-c411678e1c4a4224bd44aa3334b29af72024-12-14T06:32:18ZengElsevierMaterials Today Bio2590-00642024-12-0129101329An AIE fungal vacuole membrane probe toward species differentiation, vacuole formation visualization, and targeted photodynamic therapyBingnan Wang0Siyuan Wang1Chunyang Li2Jianqing Li3Meixi Yi4Jing-Wen Lyu5Bing Gu6Ryan T.K. Kwok7Jacky W.Y. Lam8Anjun Qin9Ben Zhong Tang10State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology, Guangzhou, 510640, China; Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, China; Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Department of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, 999077, Hong Kong, ChinaSchool of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen, (CUHK-Shenzhen), 518172, ChinaState Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology, Guangzhou, 510640, China; Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, ChinaState Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology, Guangzhou, 510640, China; Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, ChinaState Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology, Guangzhou, 510640, China; Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, ChinaDepartment of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510000, ChinaDepartment of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510000, ChinaHong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Department of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, 999077, Hong Kong, ChinaHong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Department of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, 999077, Hong Kong, ChinaState Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology, Guangzhou, 510640, China; Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, China; Corresponding author. State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou 510640, China.Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, China; School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen, (CUHK-Shenzhen), 518172, China; Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Department of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, 999077, Hong Kong, China; Corresponding author. School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, (CUHK-Shenzhen), Shenzhen 518172, China.Vacuoles are unique organelles of fungi. The development of probes targeting the vacuoles membrane will enable visualization of physiological processes and precise diagnosis and therapy. Herein, a zwitterionic molecule, MXF-R, comprising of an aggregation-induced emission (AIE) photosensitizing unit and an antibiotic moxifloxacin, was found capable of specifically imaging vacuole membrane and using for targeted antifungal therapy. MXF-R demonstrated a higher signal-to-noise ratio, stronger targeting capability, and better biocompatibility than the commercial probe FM4-64. By using MXF-R, real-time visualization of vacuole formation during Candida albicans (C. albicans) proliferation was achieved. More importantly, owing to its varying staining ability towards different fungus, MXF-R could be used to quickly identify C. albicans in mixed strains by fluorescence imaging. Moreover, MXF-R exhibits a remarkable ability to generate reactive oxygen species under white light, effectively eradicating C. albicans by disrupting membrane structure. This antifungal therapy of membrane damage is more effective than clinical drug fluconazole. Therefore, this work not only presents the initial discovery of a probe targeting vacuolar membrane, but also provides a way to develop novel materials to realize integrated diagnosis and therapy.http://www.sciencedirect.com/science/article/pii/S2590006424003909Aggregation-induced emissionVacuolar membrane probeVacuole formation visualizationFungal species differentiationPhotodynamic therapy |
| spellingShingle | Bingnan Wang Siyuan Wang Chunyang Li Jianqing Li Meixi Yi Jing-Wen Lyu Bing Gu Ryan T.K. Kwok Jacky W.Y. Lam Anjun Qin Ben Zhong Tang An AIE fungal vacuole membrane probe toward species differentiation, vacuole formation visualization, and targeted photodynamic therapy Materials Today Bio Aggregation-induced emission Vacuolar membrane probe Vacuole formation visualization Fungal species differentiation Photodynamic therapy |
| title | An AIE fungal vacuole membrane probe toward species differentiation, vacuole formation visualization, and targeted photodynamic therapy |
| title_full | An AIE fungal vacuole membrane probe toward species differentiation, vacuole formation visualization, and targeted photodynamic therapy |
| title_fullStr | An AIE fungal vacuole membrane probe toward species differentiation, vacuole formation visualization, and targeted photodynamic therapy |
| title_full_unstemmed | An AIE fungal vacuole membrane probe toward species differentiation, vacuole formation visualization, and targeted photodynamic therapy |
| title_short | An AIE fungal vacuole membrane probe toward species differentiation, vacuole formation visualization, and targeted photodynamic therapy |
| title_sort | aie fungal vacuole membrane probe toward species differentiation vacuole formation visualization and targeted photodynamic therapy |
| topic | Aggregation-induced emission Vacuolar membrane probe Vacuole formation visualization Fungal species differentiation Photodynamic therapy |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424003909 |
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