Understanding antimicrobial resistance in Campylobacter isolates from poultry environments in Gujarat, India
Campylobacter, a pathogen responsible for human gastroenteritis on a global scale, is primarily spread through the consumption of contaminated chicken meat. Antimicrobial treatments are commonly required in response to campylobacteriosis, highlighting the risk posed by antimicrobial resistance (AMR)...
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Elsevier
2025-06-01
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| Series: | Applied Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225000502 |
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| author | Sadik Dantroliya Monica Chavan Ramesh Pandit Chinmayi Joshi Fiona Tomley Damer Blake Richard Stabler Chaitanya Joshi Madhvi Joshi |
| author_facet | Sadik Dantroliya Monica Chavan Ramesh Pandit Chinmayi Joshi Fiona Tomley Damer Blake Richard Stabler Chaitanya Joshi Madhvi Joshi |
| author_sort | Sadik Dantroliya |
| collection | DOAJ |
| description | Campylobacter, a pathogen responsible for human gastroenteritis on a global scale, is primarily spread through the consumption of contaminated chicken meat. Antimicrobial treatments are commonly required in response to campylobacteriosis, highlighting the risk posed by antimicrobial resistance (AMR) in Campylobacter to food safety and public health. Monitoring and understanding AMR trends is crucial for effective risk assessment and development of management strategies. The current investigation examines the physical and genetic traits of AMR in Campylobacter species found in the caeca of chickens. Samples were collected from chicken farms and live chicken retail outlets across eight major cities in Gujarat, India. Selective culture from 750 samples found 21 of 250 samples from farms (8.4 %) and 56 of 500 samples from outlets (11.2 %) contained Campylobacter spp., confirmed by multiplex PCR and Sanger sequencing. Campylobacter coli was most common, detected in 56 samples (7.5%), with Campylobacter jejuni detected in 21 samples (2.8%). As per phenotypic assay, all the isolates were resistant to antibiotics ampicillin/sulbactam, followed by azithromycin (94.44%). Genomes were sequenced from a subset of 16 C. coli and 2 C. jejuni isolates for identification of antimicrobial resistance genes (ARGs). Eleven isolates hosted fluoroquinolone and tetracycline resistance genes. Macrolide resistance genes, such as macB, were found in 94.4% of genomes. The results of the current research highlight a high occurrence of ARG carriage in C. jejuni and C. coli, suggesting that resistance to macrolides, quinolones, and tetracyclines is common. The genotype-phenotype concordance observed was 76.39% whereas, remaining discordance (23.61%) observed was due to the six AMR genes, of which two genes were found truncated length while the remaining genes had complete lengths but had mutations. In-depth examination of the linkage between genetic and phenotypic AMR traits can support development of future strategies and policies to address the growing problem of antibiotic resistance and protect public health. |
| format | Article |
| id | doaj-art-c4082ae976f1425c9313c01e15fc5d2b |
| institution | Kabale University |
| issn | 2772-5022 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Food Research |
| spelling | doaj-art-c4082ae976f1425c9313c01e15fc5d2b2025-08-20T03:45:34ZengElsevierApplied Food Research2772-50222025-06-015110074010.1016/j.afres.2025.100740Understanding antimicrobial resistance in Campylobacter isolates from poultry environments in Gujarat, IndiaSadik Dantroliya0Monica Chavan1Ramesh Pandit2Chinmayi Joshi3Fiona Tomley4Damer Blake5Richard Stabler6Chaitanya Joshi7Madhvi Joshi8Gujarat Biotechnology Research Centre, Department of Science and Technology, Government of Gujarat, Gandhinagar 382001, Gujarat, India; Smt. S. S. Patel Nootan Science and Commerce College, Sankalchand Patel University, Visnagar, Gujarat, IndiaGujarat Biotechnology Research Centre, Department of Science and Technology, Government of Gujarat, Gandhinagar 382001, Gujarat, IndiaGujarat Biotechnology Research Centre, Department of Science and Technology, Government of Gujarat, Gandhinagar 382001, Gujarat, IndiaSmt. S. S. Patel Nootan Science and Commerce College, Sankalchand Patel University, Visnagar, Gujarat, IndiaThe Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire, AL9 7TA, United KingdomThe Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire, AL9 7TA, United KingdomDepartment of Infection Biology, London School of Hygiene & Tropical Medicine Faculty of Infectious and Tropical Diseases, London, UKGujarat Biotechnology Research Centre, Department of Science and Technology, Government of Gujarat, Gandhinagar 382001, Gujarat, IndiaGujarat Biotechnology Research Centre, Department of Science and Technology, Government of Gujarat, Gandhinagar 382001, Gujarat, India; Corresponding author at: GBRC, MS Building, 6th Floor, GH Rd, Sector 11, Gandhinagar, Gujarat 382011, India.Campylobacter, a pathogen responsible for human gastroenteritis on a global scale, is primarily spread through the consumption of contaminated chicken meat. Antimicrobial treatments are commonly required in response to campylobacteriosis, highlighting the risk posed by antimicrobial resistance (AMR) in Campylobacter to food safety and public health. Monitoring and understanding AMR trends is crucial for effective risk assessment and development of management strategies. The current investigation examines the physical and genetic traits of AMR in Campylobacter species found in the caeca of chickens. Samples were collected from chicken farms and live chicken retail outlets across eight major cities in Gujarat, India. Selective culture from 750 samples found 21 of 250 samples from farms (8.4 %) and 56 of 500 samples from outlets (11.2 %) contained Campylobacter spp., confirmed by multiplex PCR and Sanger sequencing. Campylobacter coli was most common, detected in 56 samples (7.5%), with Campylobacter jejuni detected in 21 samples (2.8%). As per phenotypic assay, all the isolates were resistant to antibiotics ampicillin/sulbactam, followed by azithromycin (94.44%). Genomes were sequenced from a subset of 16 C. coli and 2 C. jejuni isolates for identification of antimicrobial resistance genes (ARGs). Eleven isolates hosted fluoroquinolone and tetracycline resistance genes. Macrolide resistance genes, such as macB, were found in 94.4% of genomes. The results of the current research highlight a high occurrence of ARG carriage in C. jejuni and C. coli, suggesting that resistance to macrolides, quinolones, and tetracyclines is common. The genotype-phenotype concordance observed was 76.39% whereas, remaining discordance (23.61%) observed was due to the six AMR genes, of which two genes were found truncated length while the remaining genes had complete lengths but had mutations. In-depth examination of the linkage between genetic and phenotypic AMR traits can support development of future strategies and policies to address the growing problem of antibiotic resistance and protect public health.http://www.sciencedirect.com/science/article/pii/S2772502225000502Antimicrobial resistanceChickenCampylobacter spp., Genotype-phenotype concordancePoultryWhole genome sequencing |
| spellingShingle | Sadik Dantroliya Monica Chavan Ramesh Pandit Chinmayi Joshi Fiona Tomley Damer Blake Richard Stabler Chaitanya Joshi Madhvi Joshi Understanding antimicrobial resistance in Campylobacter isolates from poultry environments in Gujarat, India Applied Food Research Antimicrobial resistance Chicken Campylobacter spp., Genotype-phenotype concordance Poultry Whole genome sequencing |
| title | Understanding antimicrobial resistance in Campylobacter isolates from poultry environments in Gujarat, India |
| title_full | Understanding antimicrobial resistance in Campylobacter isolates from poultry environments in Gujarat, India |
| title_fullStr | Understanding antimicrobial resistance in Campylobacter isolates from poultry environments in Gujarat, India |
| title_full_unstemmed | Understanding antimicrobial resistance in Campylobacter isolates from poultry environments in Gujarat, India |
| title_short | Understanding antimicrobial resistance in Campylobacter isolates from poultry environments in Gujarat, India |
| title_sort | understanding antimicrobial resistance in campylobacter isolates from poultry environments in gujarat india |
| topic | Antimicrobial resistance Chicken Campylobacter spp., Genotype-phenotype concordance Poultry Whole genome sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2772502225000502 |
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