Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene
Anti-Müllerian hormone (AMH) plays an important role in avian ovarian follicle development. The high mRNA expression of AMH in avian ovarian prehierarchical follicles helps prevent premature granulosa cell differentiation. Vitamin D3 was reported to downregulate AMH mRNA expression in granulosa cell...
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124013300 |
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| author | Rong Chen Chen Chen Yifei Qin Jie Liu Mingming Lei Huanxi Zhu Zhendan Shi |
| author_facet | Rong Chen Chen Chen Yifei Qin Jie Liu Mingming Lei Huanxi Zhu Zhendan Shi |
| author_sort | Rong Chen |
| collection | DOAJ |
| description | Anti-Müllerian hormone (AMH) plays an important role in avian ovarian follicle development. The high mRNA expression of AMH in avian ovarian prehierarchical follicles helps prevent premature granulosa cell differentiation. Vitamin D3 was reported to downregulate AMH mRNA expression in granulosa cells of prehierarchical follicles in hens; however, the underlying molecular mechanism remains unknown. In this study, AMH mRNA expression was investigated in granulosa cells of prehierarchical follicles in geese under vitamin D3 induction. A potential vitamin D response element (VDRE) present in the goose AMH promoter was identified using luciferase activity, electrophoretic mobility shift assay (EMSA), and chromatin immunoprecipitation (ChIP) assays. The results showed that AMH mRNA expressions were downregulated by vitamin D3 in granulosa cells of prehierarchical follicles in geese. A classical VDRE-like sequence was identified in the goose AMH promoter by in silico analyses. Luciferase activity assays revealed that the putative VDRE is a negative regulatory element. Vitamin D receptor (VDR) and retinoic X receptor (RXR) are necessary to decrease the AMH promoter activity induced by vitamin D3. The EMSA and ChIP assays demonstrated that the VDR/RXR complex directly binds to the putative VDRE. These results suggest that vitamin D3 downregulates AMH mRNA expression via a functional VDRE that binds the VDR/RXR heterodimer in goose ovarian prehierarchical follicles. These results provide new insights into the regulatory mechanisms of vitamin D3 in avian ovarian follicular development. |
| format | Article |
| id | doaj-art-c3f1c2b8dff046e6a53af56c5eff1978 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-c3f1c2b8dff046e6a53af56c5eff19782025-01-05T04:27:44ZengElsevierPoultry Science0032-57912025-02-011042104752Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone geneRong Chen0Chen Chen1Yifei Qin2Jie Liu3Mingming Lei4Huanxi Zhu5Zhendan Shi6Key Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing, PR China; Jiangsu Province Engineering Research Center of Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, PR ChinaKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing, PR China; Jiangsu Province Engineering Research Center of Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, PR ChinaKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing, PR China; Jiangsu Province Engineering Research Center of Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, PR ChinaKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing, PR China; Jiangsu Province Engineering Research Center of Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, PR ChinaKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing, PR China; Jiangsu Province Engineering Research Center of Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, PR ChinaKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing, PR China; Jiangsu Province Engineering Research Center of Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, PR ChinaKey Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing, PR China; Jiangsu Province Engineering Research Center of Precision Animal Breeding, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, PR China; Corresponding author.Anti-Müllerian hormone (AMH) plays an important role in avian ovarian follicle development. The high mRNA expression of AMH in avian ovarian prehierarchical follicles helps prevent premature granulosa cell differentiation. Vitamin D3 was reported to downregulate AMH mRNA expression in granulosa cells of prehierarchical follicles in hens; however, the underlying molecular mechanism remains unknown. In this study, AMH mRNA expression was investigated in granulosa cells of prehierarchical follicles in geese under vitamin D3 induction. A potential vitamin D response element (VDRE) present in the goose AMH promoter was identified using luciferase activity, electrophoretic mobility shift assay (EMSA), and chromatin immunoprecipitation (ChIP) assays. The results showed that AMH mRNA expressions were downregulated by vitamin D3 in granulosa cells of prehierarchical follicles in geese. A classical VDRE-like sequence was identified in the goose AMH promoter by in silico analyses. Luciferase activity assays revealed that the putative VDRE is a negative regulatory element. Vitamin D receptor (VDR) and retinoic X receptor (RXR) are necessary to decrease the AMH promoter activity induced by vitamin D3. The EMSA and ChIP assays demonstrated that the VDR/RXR complex directly binds to the putative VDRE. These results suggest that vitamin D3 downregulates AMH mRNA expression via a functional VDRE that binds the VDR/RXR heterodimer in goose ovarian prehierarchical follicles. These results provide new insights into the regulatory mechanisms of vitamin D3 in avian ovarian follicular development.http://www.sciencedirect.com/science/article/pii/S0032579124013300GooseAnti-Müllerian hormoneVitamin D response element |
| spellingShingle | Rong Chen Chen Chen Yifei Qin Jie Liu Mingming Lei Huanxi Zhu Zhendan Shi Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene Poultry Science Goose Anti-Müllerian hormone Vitamin D response element |
| title | Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene |
| title_full | Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene |
| title_fullStr | Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene |
| title_full_unstemmed | Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene |
| title_short | Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene |
| title_sort | identification of a functional vitamin d response element in the promoter of goose anti mullerian hormone gene |
| topic | Goose Anti-Müllerian hormone Vitamin D response element |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124013300 |
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