Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis

Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis

Summary: Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Leishmania infection, harvesting infected tissues, and performing liquid chromatography-mass spectrometry (LC-MS) to identify enriched metabolic...

Full description

Saved in:
Bibliographic Details
Main Authors: Thalia Pacheco-Fernandez, Laura Klenow, Nazli Azodi, Hannah Markle, Matthew Bernier, Greta Volpedo, Timur Oljuskin, Parna Bhattacharya, Shinjiro Hamano, Greg Matlashewski, Hira L. Nakhasi, Abhay R. Satoskar, Sreenivas Gannavaram
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
metabolomics
microbiology
model organisms
molecular biology
mass spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002230
Tags: Add Tag
No Tags, Be the first to tag this record!
Be the first to leave a comment!
You must be logged in first

Similar Items