Second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis
Abstract Form-function relationships often have tradeoffs: if a material is tough, it is often inflexible, and vice versa. This is particularly relevant for the elephant trunk, where the skin should be protective yet elastic. To investigate how this is achieved, we used classical histochemical stain...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-07386-w |
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| author | Andrew K. Schulz Magdalena Plotczyk Sophia Sordilla David C. A. Gaboriau Madeline Boyle Krishma Singal Joy S. Reidenberg David L. Hu Claire A. Higgins |
| author_facet | Andrew K. Schulz Magdalena Plotczyk Sophia Sordilla David C. A. Gaboriau Madeline Boyle Krishma Singal Joy S. Reidenberg David L. Hu Claire A. Higgins |
| author_sort | Andrew K. Schulz |
| collection | DOAJ |
| description | Abstract Form-function relationships often have tradeoffs: if a material is tough, it is often inflexible, and vice versa. This is particularly relevant for the elephant trunk, where the skin should be protective yet elastic. To investigate how this is achieved, we used classical histochemical staining and second harmonic generation microscopy to describe the morphology and composition of elephant trunk skin. We report structure at the macro and micro scales, from the thickness of the dermis to the interaction of 10 μm thick collagen fibers. We analyzed several sites along the length of the trunk to compare and contrast the dorsal-ventral and proximal-distal skin morphologies and compositions. We find the dorsal skin of the elephant trunk can have keratin armor layers over 2 mm thick, which is nearly 100 times the thickness of the equivalent layer in human skin. We also found that the structural support layer (the dermis) of the elephant trunk contains a distribution of collagen-I (COL1) fibers in both perpendicular and parallel arrangement. The bimodal distribution of collagen is seen across all portions of the trunk, and is dissimilar from that of human skin where one orientation dominates within a body site. We hypothesize that this distribution of COL1 in the elephant trunk allows both flexibility and load-bearing capabilities. Additionally, when viewing individual fiber interactions of 10 μm thick collagen, we find the fiber crossings per unit volume are five times more common than in human skin, suggesting that the fibers are entangled. We surmise that these intriguing structures permit both flexibility and strength in the elephant trunk. The complex nature of the elephant skin may inspire the design of materials that can combine strength and flexibility. |
| format | Article |
| id | doaj-art-c3b40d5e4e8d4d77a4804c4201b5da32 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Communications Biology |
| spelling | doaj-art-c3b40d5e4e8d4d77a4804c4201b5da322025-01-12T12:35:42ZengNature PortfolioCommunications Biology2399-36422025-01-01811910.1038/s42003-024-07386-wSecond harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermisAndrew K. Schulz0Magdalena Plotczyk1Sophia Sordilla2David C. A. Gaboriau3Madeline Boyle4Krishma Singal5Joy S. Reidenberg6David L. Hu7Claire A. Higgins8School of Mechanical Engineering, Georgia Institute of TechnologyDepartment of Bioengineering, Imperial College LondonSchool of Biological Sciences, Georgia Institute of TechnologyFacility for Imaging by Light Microscopy, National Heart and Lung Institute, Imperial College LondonSchool of Mechanical Engineering, Georgia Institute of TechnologySchool of Physics, Georgia Institute of TechnologyCenter for Anatomy and Functional Morphology, Icahn School of Medicine at Mount SinaiSchool of Mechanical Engineering, Georgia Institute of TechnologyDepartment of Bioengineering, Imperial College LondonAbstract Form-function relationships often have tradeoffs: if a material is tough, it is often inflexible, and vice versa. This is particularly relevant for the elephant trunk, where the skin should be protective yet elastic. To investigate how this is achieved, we used classical histochemical staining and second harmonic generation microscopy to describe the morphology and composition of elephant trunk skin. We report structure at the macro and micro scales, from the thickness of the dermis to the interaction of 10 μm thick collagen fibers. We analyzed several sites along the length of the trunk to compare and contrast the dorsal-ventral and proximal-distal skin morphologies and compositions. We find the dorsal skin of the elephant trunk can have keratin armor layers over 2 mm thick, which is nearly 100 times the thickness of the equivalent layer in human skin. We also found that the structural support layer (the dermis) of the elephant trunk contains a distribution of collagen-I (COL1) fibers in both perpendicular and parallel arrangement. The bimodal distribution of collagen is seen across all portions of the trunk, and is dissimilar from that of human skin where one orientation dominates within a body site. We hypothesize that this distribution of COL1 in the elephant trunk allows both flexibility and load-bearing capabilities. Additionally, when viewing individual fiber interactions of 10 μm thick collagen, we find the fiber crossings per unit volume are five times more common than in human skin, suggesting that the fibers are entangled. We surmise that these intriguing structures permit both flexibility and strength in the elephant trunk. The complex nature of the elephant skin may inspire the design of materials that can combine strength and flexibility.https://doi.org/10.1038/s42003-024-07386-w |
| spellingShingle | Andrew K. Schulz Magdalena Plotczyk Sophia Sordilla David C. A. Gaboriau Madeline Boyle Krishma Singal Joy S. Reidenberg David L. Hu Claire A. Higgins Second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis Communications Biology |
| title | Second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis |
| title_full | Second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis |
| title_fullStr | Second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis |
| title_full_unstemmed | Second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis |
| title_short | Second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis |
| title_sort | second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis |
| url | https://doi.org/10.1038/s42003-024-07386-w |
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