Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction
The aim of this study was to determine the profile of the Luteinizing Hormone Receptor (rLH) gene in Madrasin cross breed cattle. The sample of this study used blood samples from 3 breeds of cattle. There were Madrasin from Bangkalan City, Madura from Bangkalan City and Limousin from BET Cipelang Bo...
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| Format: | Article |
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Universitas Airlangga, Faculty of Veterinary Medicine
2021-04-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/22356 |
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| author | Ivo Febrina Prasetyo Budi Utomo Sri Pantja Madyawati |
| author_facet | Ivo Febrina Prasetyo Budi Utomo Sri Pantja Madyawati |
| author_sort | Ivo Febrina Prasetyo |
| collection | DOAJ |
| description | The aim of this study was to determine the profile of the Luteinizing Hormone Receptor (rLH) gene in Madrasin cross breed cattle. The sample of this study used blood samples from 3 breeds of cattle. There were Madrasin from Bangkalan City, Madura from Bangkalan City and Limousin from BET Cipelang Bogor. The sample criteria were used female cattle around 2,5 years old and ever partus (once). The genomic DNA was extracted for polymerase chain reaction (PCR), followed by sequencing with software BioEdit Vers. 8.0 to determine the sequence of nucleotide bases. The results of this study were that three samples detected fragments in the form of DNA with nucleotide base of 373 bp. The conclusion, gene rLH in Madrasin Cattle can detected using PCR. |
| format | Article |
| id | doaj-art-c3b1e93a0e454ba1aa462b77a0d7060a |
| institution | Kabale University |
| issn | 2615-7497 2581-012X |
| language | English |
| publishDate | 2021-04-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj-art-c3b1e93a0e454ba1aa462b77a0d7060a2025-01-02T20:28:16ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2021-04-014112513010.20473/jmv.vol4.iss1.2021.125-13018302Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain ReactionIvo Febrina Prasetyo0Budi Utomo1Sri Pantja Madyawati2Proram Studi Biologi Reproduksi, Fakultas Kedokteran Hewan, Universitas AirlanggaDepatemen Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas AirlanggaDepatemen Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas AirlanggaThe aim of this study was to determine the profile of the Luteinizing Hormone Receptor (rLH) gene in Madrasin cross breed cattle. The sample of this study used blood samples from 3 breeds of cattle. There were Madrasin from Bangkalan City, Madura from Bangkalan City and Limousin from BET Cipelang Bogor. The sample criteria were used female cattle around 2,5 years old and ever partus (once). The genomic DNA was extracted for polymerase chain reaction (PCR), followed by sequencing with software BioEdit Vers. 8.0 to determine the sequence of nucleotide bases. The results of this study were that three samples detected fragments in the form of DNA with nucleotide base of 373 bp. The conclusion, gene rLH in Madrasin Cattle can detected using PCR.https://e-journal.unair.ac.id/JMV/article/view/22356genepcrrlhmadrasin cattle |
| spellingShingle | Ivo Febrina Prasetyo Budi Utomo Sri Pantja Madyawati Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction Jurnal Medik Veteriner gene pcr rlh madrasin cattle |
| title | Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction |
| title_full | Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction |
| title_fullStr | Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction |
| title_full_unstemmed | Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction |
| title_short | Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction |
| title_sort | detection gene of luteinizing hormone receptor rlh in madrasin cattle using polymerase chain reaction |
| topic | gene pcr rlh madrasin cattle |
| url | https://e-journal.unair.ac.id/JMV/article/view/22356 |
| work_keys_str_mv | AT ivofebrinaprasetyo detectiongeneofluteinizinghormonereceptorrlhinmadrasincattleusingpolymerasechainreaction AT budiutomo detectiongeneofluteinizinghormonereceptorrlhinmadrasincattleusingpolymerasechainreaction AT sripantjamadyawati detectiongeneofluteinizinghormonereceptorrlhinmadrasincattleusingpolymerasechainreaction |