Determination of melamine in biscuits by high performance liquid chromatography
A high performance liquid chromatography method was developed for the determination of melamine in biscuits. The biscuit samples were extracted by 1% trichloroacetic acid solution and 2.2% lead acetate, and melamine was separated on a Hypersil ODS C18 column (100 mm×4.6 mm, 5 μm). Acetonitrile and s...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.01.019 |
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| Summary: | A high performance liquid chromatography method was developed for the determination of melamine in biscuits. The biscuit samples were extracted by 1% trichloroacetic acid solution and 2.2% lead acetate, and melamine was separated on a Hypersil ODS C18 column (100 mm×4.6 mm, 5 μm). Acetonitrile and sodium 1-octanesulfonate solution(pH 3) (15∶85, V/V) were used as mobile phase at a flow rate of 1.0 mL·min<sup>-1</sup>. The detective wavelength was set as 210 nm by an ultraviolet detector. The temperature of column was 40 ℃. The linear ranges for melamine were 0.2-10 μg·mL<sup>-1</sup>. The average recovery rate of this method varied from 80.5% to 93.5% with the relative standard deviation of 7.7% (n=9) when blank sample was fortified standard working solution of 1, 2 and 4 μg·mL<sup>-1</sup>. |
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| ISSN: | 1008-9209 2097-5155 |