Population structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chip

Abstract The frigid and droughty climate of Xinjiang in China has given rise to unique indigenous sheep breeds with robust adaptability and resistance. To investigate the genetic mechanism of adaptability of Xinjiang sheep to the local extreme environment, we conducted population genetic structure a...

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Main Authors: Yanhao Li, Xiaopeng Li, Zhipeng Han, Ruizhi Yang, Wen Zhou, Yuwei Peng, Jianzhong He, Shudong Liu
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-76573-w
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author Yanhao Li
Xiaopeng Li
Zhipeng Han
Ruizhi Yang
Wen Zhou
Yuwei Peng
Jianzhong He
Shudong Liu
author_facet Yanhao Li
Xiaopeng Li
Zhipeng Han
Ruizhi Yang
Wen Zhou
Yuwei Peng
Jianzhong He
Shudong Liu
author_sort Yanhao Li
collection DOAJ
description Abstract The frigid and droughty climate of Xinjiang in China has given rise to unique indigenous sheep breeds with robust adaptability and resistance. To investigate the genetic mechanism of adaptability of Xinjiang sheep to the local extreme environment, we conducted population genetic structure analyses for three native Xinjiang sheep breeds: Altay sheep (ALT), Bashbay Sheep (BSBC), and Duolang sheep (DLC), as well as two foreign sheep breeds: Suffolk and Dorset, using the Ovine Infinium HD SNP BeadChip(680 K). Our findings revealed distinct genetic and evolutionary histories between Xinjiang and foreign sheep breeds. Principal Component Analysis (PCA) and phylogenetic tree effectively differentiate these five sheep breeds based on their geographical origins, and the domestication level of Xinjiang sheep is comparatively lower than that of foreign sheep breeds. Furthermore, by utilizing three selective signature methods, namely Fixation Index (Fst), Cross Population Extended Haplotype Homozygosity Test (XP-EHH), and Nucleotide Diversity (π), we have successfully identified 22 potential candidate genes. Among these genes, there are TBXT, PDGFD, and VEGFA, which are closely related to tail type and lipid metabolism; VIL1, SLC11A1, and ZBTB46, which are associated with immune function; and candidate genes such as BNC1, HDAC1, and BMP5, which impact sheep reproductive traits. This study establishes a foundation for conserving and utilizing local sheep germplasm resources in Xinjiang and provides molecular insights into the genetic mechanisms governing sheep adaptation to extreme cold and arid environments.
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spelling doaj-art-c38e5f72597e42cba8d3a626763423e92024-11-17T12:22:35ZengNature PortfolioScientific Reports2045-23222024-11-0114111410.1038/s41598-024-76573-wPopulation structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chipYanhao Li0Xiaopeng Li1Zhipeng Han2Ruizhi Yang3Wen Zhou4Yuwei Peng5Jianzhong He6Shudong Liu7College of Animal Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityKey Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction CorpsCollege of Animal Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityCollege of Animal Science and Technology, Tarim UniversityAbstract The frigid and droughty climate of Xinjiang in China has given rise to unique indigenous sheep breeds with robust adaptability and resistance. To investigate the genetic mechanism of adaptability of Xinjiang sheep to the local extreme environment, we conducted population genetic structure analyses for three native Xinjiang sheep breeds: Altay sheep (ALT), Bashbay Sheep (BSBC), and Duolang sheep (DLC), as well as two foreign sheep breeds: Suffolk and Dorset, using the Ovine Infinium HD SNP BeadChip(680 K). Our findings revealed distinct genetic and evolutionary histories between Xinjiang and foreign sheep breeds. Principal Component Analysis (PCA) and phylogenetic tree effectively differentiate these five sheep breeds based on their geographical origins, and the domestication level of Xinjiang sheep is comparatively lower than that of foreign sheep breeds. Furthermore, by utilizing three selective signature methods, namely Fixation Index (Fst), Cross Population Extended Haplotype Homozygosity Test (XP-EHH), and Nucleotide Diversity (π), we have successfully identified 22 potential candidate genes. Among these genes, there are TBXT, PDGFD, and VEGFA, which are closely related to tail type and lipid metabolism; VIL1, SLC11A1, and ZBTB46, which are associated with immune function; and candidate genes such as BNC1, HDAC1, and BMP5, which impact sheep reproductive traits. This study establishes a foundation for conserving and utilizing local sheep germplasm resources in Xinjiang and provides molecular insights into the genetic mechanisms governing sheep adaptation to extreme cold and arid environments.https://doi.org/10.1038/s41598-024-76573-wSheepSNP chipPopulation genetic structureSelective sweep
spellingShingle Yanhao Li
Xiaopeng Li
Zhipeng Han
Ruizhi Yang
Wen Zhou
Yuwei Peng
Jianzhong He
Shudong Liu
Population structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chip
Scientific Reports
Sheep
SNP chip
Population genetic structure
Selective sweep
title Population structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chip
title_full Population structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chip
title_fullStr Population structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chip
title_full_unstemmed Population structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chip
title_short Population structure and selective signature analysis of local sheep breeds in Xinjiang, China based on high-density SNP chip
title_sort population structure and selective signature analysis of local sheep breeds in xinjiang china based on high density snp chip
topic Sheep
SNP chip
Population genetic structure
Selective sweep
url https://doi.org/10.1038/s41598-024-76573-w
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