Whole genome analysis, detoxification of ochratoxin a and physiological characterization of a novel Bacillus velezensis MM35 isolated from soil
Ochratoxin A (OTA) is a significant global contaminant that poses severe challenges to food safety and public health. This study aims to isolate the OTA-degrated probiotics and evaluate genetic and biological characteristic. Here, The degradation rate of a new strain named Bacillus velezensis MM35 i...
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Frontiers Media S.A.
2024-12-01
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| author | Fengru Xu Fengru Xu Fengru Xu Mengmeng Tang Mengmeng Tang Mengmeng Tang Zhihao Yang Zhihao Yang Zhihao Yang Chengshui Liao Chengshui Liao Chengshui Liao Zuhua Yu Zuhua Yu Zuhua Yu Rongxian Guo Rongxian Guo Rongxian Guo Ke Shang Ke Shang Ke Shang Songbiao Chen Songbiao Chen Songbiao Chen Ke Yang Ke Yang Ke Yang Jing Li Jing Li Jing Li Ke Ding Ke Ding Ke Ding Yanyan Jia Yanyan Jia Yanyan Jia |
| author_facet | Fengru Xu Fengru Xu Fengru Xu Mengmeng Tang Mengmeng Tang Mengmeng Tang Zhihao Yang Zhihao Yang Zhihao Yang Chengshui Liao Chengshui Liao Chengshui Liao Zuhua Yu Zuhua Yu Zuhua Yu Rongxian Guo Rongxian Guo Rongxian Guo Ke Shang Ke Shang Ke Shang Songbiao Chen Songbiao Chen Songbiao Chen Ke Yang Ke Yang Ke Yang Jing Li Jing Li Jing Li Ke Ding Ke Ding Ke Ding Yanyan Jia Yanyan Jia Yanyan Jia |
| author_sort | Fengru Xu |
| collection | DOAJ |
| description | Ochratoxin A (OTA) is a significant global contaminant that poses severe challenges to food safety and public health. This study aims to isolate the OTA-degrated probiotics and evaluate genetic and biological characteristic. Here, The degradation rate of a new strain named Bacillus velezensis MM35 isolated from soil was the highest (87.10% within 48 h), and its culture supernatant was the main component of OTA degradation (63.95%) by high performance liquid chromatography. Further investigation revealed that the extracellular enzyme that degrades OTA in the culture supernatant of MM35 may be a small molecule enzyme with certain heat resistance. Genome-wide analysis showed that MM35 contains a cluster of carboxypeptidases encoding OTA-degrading potential, and had good metabolic and catalytic synthesis ability, and strong application potential in the synthesis and degradation of carbohydrates and proteins. A variety of secondary metabolites with antibacterial properties, such as non-ribosomal peptide synthetase and terpenoids, were identified in its metabolites. Consistent with the predicted results, MM35 showed various enzyme production characteristics such as cellulase and xylanase. Furthermore, MM35 could inhibit the growth of a variety of pathogenic bacteria, and showed high co-aggregation ability to Escherichia coli and Salmonella typhimurium. In addition, MM35 has certain tolerance to harsh environments such as strong acid, bile salt, and high temperature. Additionally, the adhesion rate of MM35 was 5.4%, and the invasion rate was 2.1% in IPEC-J2 cells. In summary, the data suggest MM35 isolated strain has high OTA degradation efficiency, antibacterial activity and intestinal colonization, which provided a new way for the treatment of OTA contamination in food and feed industries. |
| format | Article |
| id | doaj-art-c38b73caa9b347688efacfdfd36b97ef |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-c38b73caa9b347688efacfdfd36b97ef2024-12-18T06:43:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.14978601497860Whole genome analysis, detoxification of ochratoxin a and physiological characterization of a novel Bacillus velezensis MM35 isolated from soilFengru Xu0Fengru Xu1Fengru Xu2Mengmeng Tang3Mengmeng Tang4Mengmeng Tang5Zhihao Yang6Zhihao Yang7Zhihao Yang8Chengshui Liao9Chengshui Liao10Chengshui Liao11Zuhua Yu12Zuhua Yu13Zuhua Yu14Rongxian Guo15Rongxian Guo16Rongxian Guo17Ke Shang18Ke Shang19Ke Shang20Songbiao Chen21Songbiao Chen22Songbiao Chen23Ke Yang24Ke Yang25Ke Yang26Jing Li27Jing Li28Jing Li29Ke Ding30Ke Ding31Ke Ding32Yanyan Jia33Yanyan Jia34Yanyan Jia35College of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, ChinaThe Key Lab of Animal Disease and Public Health, Henan University of Science and Technology, Luoyang, ChinaOchratoxin A (OTA) is a significant global contaminant that poses severe challenges to food safety and public health. This study aims to isolate the OTA-degrated probiotics and evaluate genetic and biological characteristic. Here, The degradation rate of a new strain named Bacillus velezensis MM35 isolated from soil was the highest (87.10% within 48 h), and its culture supernatant was the main component of OTA degradation (63.95%) by high performance liquid chromatography. Further investigation revealed that the extracellular enzyme that degrades OTA in the culture supernatant of MM35 may be a small molecule enzyme with certain heat resistance. Genome-wide analysis showed that MM35 contains a cluster of carboxypeptidases encoding OTA-degrading potential, and had good metabolic and catalytic synthesis ability, and strong application potential in the synthesis and degradation of carbohydrates and proteins. A variety of secondary metabolites with antibacterial properties, such as non-ribosomal peptide synthetase and terpenoids, were identified in its metabolites. Consistent with the predicted results, MM35 showed various enzyme production characteristics such as cellulase and xylanase. Furthermore, MM35 could inhibit the growth of a variety of pathogenic bacteria, and showed high co-aggregation ability to Escherichia coli and Salmonella typhimurium. In addition, MM35 has certain tolerance to harsh environments such as strong acid, bile salt, and high temperature. Additionally, the adhesion rate of MM35 was 5.4%, and the invasion rate was 2.1% in IPEC-J2 cells. In summary, the data suggest MM35 isolated strain has high OTA degradation efficiency, antibacterial activity and intestinal colonization, which provided a new way for the treatment of OTA contamination in food and feed industries.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1497860/fullochratoxin AbiodegradationBacillus velezensiswhole genome analysisphysiological characterization |
| spellingShingle | Fengru Xu Fengru Xu Fengru Xu Mengmeng Tang Mengmeng Tang Mengmeng Tang Zhihao Yang Zhihao Yang Zhihao Yang Chengshui Liao Chengshui Liao Chengshui Liao Zuhua Yu Zuhua Yu Zuhua Yu Rongxian Guo Rongxian Guo Rongxian Guo Ke Shang Ke Shang Ke Shang Songbiao Chen Songbiao Chen Songbiao Chen Ke Yang Ke Yang Ke Yang Jing Li Jing Li Jing Li Ke Ding Ke Ding Ke Ding Yanyan Jia Yanyan Jia Yanyan Jia Whole genome analysis, detoxification of ochratoxin a and physiological characterization of a novel Bacillus velezensis MM35 isolated from soil Frontiers in Microbiology ochratoxin A biodegradation Bacillus velezensis whole genome analysis physiological characterization |
| title | Whole genome analysis, detoxification of ochratoxin a and physiological characterization of a novel Bacillus velezensis MM35 isolated from soil |
| title_full | Whole genome analysis, detoxification of ochratoxin a and physiological characterization of a novel Bacillus velezensis MM35 isolated from soil |
| title_fullStr | Whole genome analysis, detoxification of ochratoxin a and physiological characterization of a novel Bacillus velezensis MM35 isolated from soil |
| title_full_unstemmed | Whole genome analysis, detoxification of ochratoxin a and physiological characterization of a novel Bacillus velezensis MM35 isolated from soil |
| title_short | Whole genome analysis, detoxification of ochratoxin a and physiological characterization of a novel Bacillus velezensis MM35 isolated from soil |
| title_sort | whole genome analysis detoxification of ochratoxin a and physiological characterization of a novel bacillus velezensis mm35 isolated from soil |
| topic | ochratoxin A biodegradation Bacillus velezensis whole genome analysis physiological characterization |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1497860/full |
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