Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity
This study evaluated the potential impact of VCO-loaded liposomes, particularly on the activation of tyrosinase. Optimized liposomes containing 1 % (w/w) VCO were prepared using the film deposition on carrier method, resulting in a particle size of 84.02 ± 5.00 nm and a zeta potential of -68.40 ± 2....
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | OpenNano |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352952025000015 |
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| author | Suwipa Ungphaiboon Sutasinee Ardhanwanich Duangkhae Maneenuan Sirirat Pinsuwan Pawika Mahasawat |
| author_facet | Suwipa Ungphaiboon Sutasinee Ardhanwanich Duangkhae Maneenuan Sirirat Pinsuwan Pawika Mahasawat |
| author_sort | Suwipa Ungphaiboon |
| collection | DOAJ |
| description | This study evaluated the potential impact of VCO-loaded liposomes, particularly on the activation of tyrosinase. Optimized liposomes containing 1 % (w/w) VCO were prepared using the film deposition on carrier method, resulting in a particle size of 84.02 ± 5.00 nm and a zeta potential of -68.40 ± 2.78 mV. Encapsulation of VCO enhanced mushroom tyrosinase activity by 3-fold and exhibited lower cytotoxicity to B16-F10 cells compared to VCO alone. Moreover, a positive correlation was observed between the increase in intracellular tyrosinase activity and the concentrations of VCO (r = 0.8366) and VCO-loaded liposomes (r = 0.4794) in B16-F10 cells, while a negative correlation (r = -0.0545) was found for liposomes without VCO. A hair and eyebrow-darkening gel containing both VCO and VCO-loaded liposomes further enhanced mushroom tyrosinase activity by 283.33 ± 26.58 %. These findings suggest that VCO-loaded liposomes may serve as novel and effective nano-scale carriers for VCO in cosmetic applications. |
| format | Article |
| id | doaj-art-c3875b84773e4596b988c313be4e8af0 |
| institution | Kabale University |
| issn | 2352-9520 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | OpenNano |
| spelling | doaj-art-c3875b84773e4596b988c313be4e8af02025-01-08T04:53:06ZengElsevierOpenNano2352-95202025-03-0122100232Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activitySuwipa Ungphaiboon0Sutasinee Ardhanwanich1Duangkhae Maneenuan2Sirirat Pinsuwan3Pawika Mahasawat4Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, Thailand; Drug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, ThailandDepartment of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, ThailandDepartment of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, Thailand; Drug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, ThailandDepartment of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, ThailandProgram of Biotechnology, Faculty of Science and Technology, Songkhla Rajabhat University, Songkhla, Thailand; Corresponding author.This study evaluated the potential impact of VCO-loaded liposomes, particularly on the activation of tyrosinase. Optimized liposomes containing 1 % (w/w) VCO were prepared using the film deposition on carrier method, resulting in a particle size of 84.02 ± 5.00 nm and a zeta potential of -68.40 ± 2.78 mV. Encapsulation of VCO enhanced mushroom tyrosinase activity by 3-fold and exhibited lower cytotoxicity to B16-F10 cells compared to VCO alone. Moreover, a positive correlation was observed between the increase in intracellular tyrosinase activity and the concentrations of VCO (r = 0.8366) and VCO-loaded liposomes (r = 0.4794) in B16-F10 cells, while a negative correlation (r = -0.0545) was found for liposomes without VCO. A hair and eyebrow-darkening gel containing both VCO and VCO-loaded liposomes further enhanced mushroom tyrosinase activity by 283.33 ± 26.58 %. These findings suggest that VCO-loaded liposomes may serve as novel and effective nano-scale carriers for VCO in cosmetic applications.http://www.sciencedirect.com/science/article/pii/S2352952025000015Virgin coconut oil (VCO)TyrosinaseLiposomesCosmetics |
| spellingShingle | Suwipa Ungphaiboon Sutasinee Ardhanwanich Duangkhae Maneenuan Sirirat Pinsuwan Pawika Mahasawat Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity OpenNano Virgin coconut oil (VCO) Tyrosinase Liposomes Cosmetics |
| title | Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity |
| title_full | Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity |
| title_fullStr | Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity |
| title_full_unstemmed | Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity |
| title_short | Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity |
| title_sort | evaluation of nano sized virgin coconut oil vco loaded liposomes for enhancing mushroom and b16 f10 tyrosinase activity |
| topic | Virgin coconut oil (VCO) Tyrosinase Liposomes Cosmetics |
| url | http://www.sciencedirect.com/science/article/pii/S2352952025000015 |
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