Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo
Polyphenols are potential neuroprotectors that increase lifespan and slow down aging. Red clover (Trifolium pratense L.) is a promising source of biologically active substances. Its extracts contain biochanin A and chlorogenic acid. This research used Caenorhabditis elegans to study the effect of po...
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Kemerovo State University
2023-12-01
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| Series: | Техника и технология пищевых производств |
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| Online Access: | https://fptt.ru/en/issues/22269/22242/ |
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| author | Irina S. Milentyeva Anna D. Vesnina Anastasiya M. Fedorova Elena V. Ostapova Timothy A. Larichev |
| author_facet | Irina S. Milentyeva Anna D. Vesnina Anastasiya M. Fedorova Elena V. Ostapova Timothy A. Larichev |
| author_sort | Irina S. Milentyeva |
| collection | DOAJ |
| description | Polyphenols are potential neuroprotectors that increase lifespan and slow down aging. Red clover (Trifolium pratense L.) is a promising source of biologically active substances. Its extracts contain biochanin A and chlorogenic acid. This research used Caenorhabditis elegans to study the effect of polyphenols extracted from red clover callus cultures on SOD-3 and HSP-16.2 genes, as well as their anti-amyloid potential. The chlorogenic acid and biochanin A (200, 100, 50, and 10 µM) with a purity of 95% were isolated from callus extracts of T. pratense L. The effect of polyphenols on SOD-3 and HSP-16.2 was assessed after 5 and 2 h of heat stress (35°C), respectively, using C. elegans N2 Bristol as model organism. The neuroprotective potential was measured by counting paralyzed nematodes after 18, 40, and 62 h of incubation. The research established a dose-dependent effect between the concentration of biologically active substances and the percentage of paralyzed nematodes after 18 h of cultivation. The lowest paralysis phenotype count occurred at a concentration of 200 μM. The activity of 200 μM biochnin A was 1.18 times as high as that of a 200 μM chlorogenic acid solution. Biochanin A solutions increased SOD-3 expression by 3.7 times, compared to the control. The biologically active substances exhibited relative neuroprotective activity and affected the expression of antioxidant defense gene in C. elegans. |
| format | Article |
| id | doaj-art-c3757d9a720f43b0bb9c62559c5382b1 |
| institution | Kabale University |
| issn | 2074-9414 2313-1748 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Техника и технология пищевых производств |
| spelling | doaj-art-c3757d9a720f43b0bb9c62559c5382b12025-01-03T00:16:45ZengKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482023-12-0153475476510.21603/2074-9414-2023-4-2475Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In VivoIrina S. Milentyeva0https://orcid.org/0000-0002-3536-562XAnna D. Vesnina1https://orcid.org/0000-0002-4552-7418Anastasiya M. Fedorova2https://orcid.org/0000-0002-8071-4411Elena V. Ostapova3https://orcid.org/0000-0002-4704-484XTimothy A. Larichev4https://orcid.org/0000-0003-0166-2527Kemerovo State University , Kemerovo, RussiaKemerovo State University , Kemerovo, RussiaKemerovo State University , Kemerovo, RussiaKemerovo State University , Kemerovo, RussiaKemerovo State University , Kemerovo, RussiaPolyphenols are potential neuroprotectors that increase lifespan and slow down aging. Red clover (Trifolium pratense L.) is a promising source of biologically active substances. Its extracts contain biochanin A and chlorogenic acid. This research used Caenorhabditis elegans to study the effect of polyphenols extracted from red clover callus cultures on SOD-3 and HSP-16.2 genes, as well as their anti-amyloid potential. The chlorogenic acid and biochanin A (200, 100, 50, and 10 µM) with a purity of 95% were isolated from callus extracts of T. pratense L. The effect of polyphenols on SOD-3 and HSP-16.2 was assessed after 5 and 2 h of heat stress (35°C), respectively, using C. elegans N2 Bristol as model organism. The neuroprotective potential was measured by counting paralyzed nematodes after 18, 40, and 62 h of incubation. The research established a dose-dependent effect between the concentration of biologically active substances and the percentage of paralyzed nematodes after 18 h of cultivation. The lowest paralysis phenotype count occurred at a concentration of 200 μM. The activity of 200 μM biochnin A was 1.18 times as high as that of a 200 μM chlorogenic acid solution. Biochanin A solutions increased SOD-3 expression by 3.7 times, compared to the control. The biologically active substances exhibited relative neuroprotective activity and affected the expression of antioxidant defense gene in C. elegans.https://fptt.ru/en/issues/22269/22242/agingalzheimer's diseaseneuroprotectorspolyphenolschlorogenic acidbiochanin acaenorhabditis elegansβ-amyloid peptide |
| spellingShingle | Irina S. Milentyeva Anna D. Vesnina Anastasiya M. Fedorova Elena V. Ostapova Timothy A. Larichev Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo Техника и технология пищевых производств aging alzheimer's disease neuroprotectors polyphenols chlorogenic acid biochanin a caenorhabditis elegans β-amyloid peptide |
| title | Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo |
| title_full | Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo |
| title_fullStr | Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo |
| title_full_unstemmed | Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo |
| title_short | Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo |
| title_sort | chlorogenic acid and biohanin a from trifolium pratense l callus culture extract functional activity in vivo |
| topic | aging alzheimer's disease neuroprotectors polyphenols chlorogenic acid biochanin a caenorhabditis elegans β-amyloid peptide |
| url | https://fptt.ru/en/issues/22269/22242/ |
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