Protocol for fabricating elastomeric stencils for patterned stem cell differentiation
Summary: Geometrically controlled stem cell differentiation promotes reproducible pattern formation. Here, we present a protocol to fabricate elastomeric stencils for patterned stem cell differentiation. We describe procedures for using photolithography to produce molds, followed by molding polydime...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724003526 |
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| author | Stefanie Lehr Jack Merrin Monika Kulig Thomas George Minchington Anna Kicheva |
| author_facet | Stefanie Lehr Jack Merrin Monika Kulig Thomas George Minchington Anna Kicheva |
| author_sort | Stefanie Lehr |
| collection | DOAJ |
| description | Summary: Geometrically controlled stem cell differentiation promotes reproducible pattern formation. Here, we present a protocol to fabricate elastomeric stencils for patterned stem cell differentiation. We describe procedures for using photolithography to produce molds, followed by molding polydimethylsiloxane (PDMS) to obtain stencils with through holes. We then provide instructions for culturing cells on stencils and, finally, removing stencils to allow colony growth and cell migration. This approach yields reproducible two-dimensional organoids tailored for quantitative studies of growth and pattern formation.For complete details on the use and execution of this protocol, please refer to Lehr et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-c34e49a81b8b452f94d1a29d8e6a8e47 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-c34e49a81b8b452f94d1a29d8e6a8e472024-11-27T05:03:11ZengElsevierSTAR Protocols2666-16672024-12-0154103187Protocol for fabricating elastomeric stencils for patterned stem cell differentiationStefanie Lehr0Jack Merrin1Monika Kulig2Thomas George Minchington3Anna Kicheva4Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, AustriaInstitute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, AustriaInstitute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, AustriaInstitute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, AustriaInstitute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria; Corresponding authorSummary: Geometrically controlled stem cell differentiation promotes reproducible pattern formation. Here, we present a protocol to fabricate elastomeric stencils for patterned stem cell differentiation. We describe procedures for using photolithography to produce molds, followed by molding polydimethylsiloxane (PDMS) to obtain stencils with through holes. We then provide instructions for culturing cells on stencils and, finally, removing stencils to allow colony growth and cell migration. This approach yields reproducible two-dimensional organoids tailored for quantitative studies of growth and pattern formation.For complete details on the use and execution of this protocol, please refer to Lehr et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724003526Developmental biologyCell DifferentiationOrganoidsBiotechnology and bioengineering |
| spellingShingle | Stefanie Lehr Jack Merrin Monika Kulig Thomas George Minchington Anna Kicheva Protocol for fabricating elastomeric stencils for patterned stem cell differentiation STAR Protocols Developmental biology Cell Differentiation Organoids Biotechnology and bioengineering |
| title | Protocol for fabricating elastomeric stencils for patterned stem cell differentiation |
| title_full | Protocol for fabricating elastomeric stencils for patterned stem cell differentiation |
| title_fullStr | Protocol for fabricating elastomeric stencils for patterned stem cell differentiation |
| title_full_unstemmed | Protocol for fabricating elastomeric stencils for patterned stem cell differentiation |
| title_short | Protocol for fabricating elastomeric stencils for patterned stem cell differentiation |
| title_sort | protocol for fabricating elastomeric stencils for patterned stem cell differentiation |
| topic | Developmental biology Cell Differentiation Organoids Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724003526 |
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