Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatment

The overuse and misuse of antibiotics have accelerated the selection of antibiotic-resistant bacteria, significantly impacting human, animal, and environmental health. As aquatic environments are vulnerable to antibiotic resistance, suitable management practices should be adopted to tackle this phen...

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Main Authors: Lluís Bertrans-Tubau, Sergio Martínez-Campos, Julio Lopez-Doval, Meritxell Abril, Sergio Ponsá, Victoria Salvadó, Manuela Hidalgo, Anna Pico-Tomàs, Jose Luis Balcazar, Lorenzo Proia
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Environmental Science and Ecotechnology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666498424000590
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author Lluís Bertrans-Tubau
Sergio Martínez-Campos
Julio Lopez-Doval
Meritxell Abril
Sergio Ponsá
Victoria Salvadó
Manuela Hidalgo
Anna Pico-Tomàs
Jose Luis Balcazar
Lorenzo Proia
author_facet Lluís Bertrans-Tubau
Sergio Martínez-Campos
Julio Lopez-Doval
Meritxell Abril
Sergio Ponsá
Victoria Salvadó
Manuela Hidalgo
Anna Pico-Tomàs
Jose Luis Balcazar
Lorenzo Proia
author_sort Lluís Bertrans-Tubau
collection DOAJ
description The overuse and misuse of antibiotics have accelerated the selection of antibiotic-resistant bacteria, significantly impacting human, animal, and environmental health. As aquatic environments are vulnerable to antibiotic resistance, suitable management practices should be adopted to tackle this phenomenon. Here we show an effective, nature-based solution for reducing antibiotic resistance from actual wastewater. We utilize a bioreactor that relies on benthic (biofilms) and planktonic microbial communities to treat secondary effluent from a small urban wastewater treatment plant (<10,000 population equivalent). This treated effluent is eventually released into the local aquatic ecosystem. We observe high removal efficiency for genes that provide resistance to commonly used antibiotic families, as well as for mobile genetic elements that could potentially aid in their spread. Importantly, we notice a buildup of sulfonamide (sul1 and sul2) and tetracycline (tet(C), tet(G), and tetR) resistance genes specifically in biofilms. This advancement marks the initial step in considering this bioreactor as a nature-based, cost-effective tertiary treatment option for small UWWTPs facing antibiotic resistance challenges.
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institution Kabale University
issn 2666-4984
language English
publishDate 2024-11-01
publisher Elsevier
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series Environmental Science and Ecotechnology
spelling doaj-art-c34799d52f5d4f4b9f983a2d1ac06c2d2024-11-15T06:15:12ZengElsevierEnvironmental Science and Ecotechnology2666-49842024-11-0122100445Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatmentLluís Bertrans-Tubau0Sergio Martínez-Campos1Julio Lopez-Doval2Meritxell Abril3Sergio Ponsá4Victoria Salvadó5Manuela Hidalgo6Anna Pico-Tomàs7Jose Luis Balcazar8Lorenzo Proia9BETA Technological Centre- University of Vic- Central University of Catalunya (BETA- UVIC- UCC), Carretera de Roda 70, 08500, Vic, Barcelona, SpainBETA Technological Centre- University of Vic- Central University of Catalunya (BETA- UVIC- UCC), Carretera de Roda 70, 08500, Vic, Barcelona, SpainBETA Technological Centre- University of Vic- Central University of Catalunya (BETA- UVIC- UCC), Carretera de Roda 70, 08500, Vic, Barcelona, SpainBETA Technological Centre- University of Vic- Central University of Catalunya (BETA- UVIC- UCC), Carretera de Roda 70, 08500, Vic, Barcelona, SpainBETA Technological Centre- University of Vic- Central University of Catalunya (BETA- UVIC- UCC), Carretera de Roda 70, 08500, Vic, Barcelona, SpainChemistry Department, University of Girona. Campus Montilivi, 17005, Girona, SpainChemistry Department, University of Girona. Campus Montilivi, 17005, Girona, SpainCatalan Institute Water Research (ICRA-CERCA), Emili Grahit 101, 17003, Girona, SpainCatalan Institute Water Research (ICRA-CERCA), Emili Grahit 101, 17003, Girona, Spain; University of Girona, 17004, Girona, SpainBETA Technological Centre- University of Vic- Central University of Catalunya (BETA- UVIC- UCC), Carretera de Roda 70, 08500, Vic, Barcelona, Spain; Corresponding author.The overuse and misuse of antibiotics have accelerated the selection of antibiotic-resistant bacteria, significantly impacting human, animal, and environmental health. As aquatic environments are vulnerable to antibiotic resistance, suitable management practices should be adopted to tackle this phenomenon. Here we show an effective, nature-based solution for reducing antibiotic resistance from actual wastewater. We utilize a bioreactor that relies on benthic (biofilms) and planktonic microbial communities to treat secondary effluent from a small urban wastewater treatment plant (<10,000 population equivalent). This treated effluent is eventually released into the local aquatic ecosystem. We observe high removal efficiency for genes that provide resistance to commonly used antibiotic families, as well as for mobile genetic elements that could potentially aid in their spread. Importantly, we notice a buildup of sulfonamide (sul1 and sul2) and tetracycline (tet(C), tet(G), and tetR) resistance genes specifically in biofilms. This advancement marks the initial step in considering this bioreactor as a nature-based, cost-effective tertiary treatment option for small UWWTPs facing antibiotic resistance challenges.http://www.sciencedirect.com/science/article/pii/S2666498424000590ARGsMGEsUWWTPsNBSBiofilms
spellingShingle Lluís Bertrans-Tubau
Sergio Martínez-Campos
Julio Lopez-Doval
Meritxell Abril
Sergio Ponsá
Victoria Salvadó
Manuela Hidalgo
Anna Pico-Tomàs
Jose Luis Balcazar
Lorenzo Proia
Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatment
Environmental Science and Ecotechnology
ARGs
MGEs
UWWTPs
NBS
Biofilms
title Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatment
title_full Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatment
title_fullStr Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatment
title_full_unstemmed Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatment
title_short Nature-based bioreactors: Tackling antibiotic resistance in urban wastewater treatment
title_sort nature based bioreactors tackling antibiotic resistance in urban wastewater treatment
topic ARGs
MGEs
UWWTPs
NBS
Biofilms
url http://www.sciencedirect.com/science/article/pii/S2666498424000590
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