Neuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat diet
ObjectiveEnergy homeostasis is modulated by the hypothalamic is essential for obesity progression, however, the gene expression profiling remains to be fully understood.MethodsGEO datasets were downloaded from the GEO website and analyzed by the R packages to obtain the DEGs. And, the WGCNA analysis...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2024.1443880/full |
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| author | Caixia Liang Caixia Liang Caixia Liang Caixia Liang Hongjian Lu Xueqin Wang Jianbin Su Feng Qi Yanxing Shang Yanxing Shang Yanxing Shang Yanxing Shang Yu Li Dongmei Zhang Dongmei Zhang Dongmei Zhang Dongmei Zhang Dongmei Zhang Chengwei Duan Chengwei Duan Chengwei Duan Chengwei Duan |
| author_facet | Caixia Liang Caixia Liang Caixia Liang Caixia Liang Hongjian Lu Xueqin Wang Jianbin Su Feng Qi Yanxing Shang Yanxing Shang Yanxing Shang Yanxing Shang Yu Li Dongmei Zhang Dongmei Zhang Dongmei Zhang Dongmei Zhang Dongmei Zhang Chengwei Duan Chengwei Duan Chengwei Duan Chengwei Duan |
| author_sort | Caixia Liang |
| collection | DOAJ |
| description | ObjectiveEnergy homeostasis is modulated by the hypothalamic is essential for obesity progression, however, the gene expression profiling remains to be fully understood.MethodsGEO datasets were downloaded from the GEO website and analyzed by the R packages to obtain the DEGs. And, the WGCNA analysis and PPI networks of co-expressed DEGs were designed using STRING to get key genes. In addition, the single-cell sequencing datasets and GTEx database were utilized to receive the neuron-stress genes from the key genes. Further, high-fat diet (HFD)-induced hypothalamic tissue of mice was used as an animal model to validate the mRNA up-regulation of neuron-stress genes. In addition, the Bmi1 gene was identified as a hub gene through the LASSO model and nomogram analysis. Western blot confirmed the high expression of Bmi1 in hypothalamic tissue of HFD mice and PA-stimulated microglia. Immunofluorescence staining showed that HFD induced the activation of microglia and the expression of Bmi1 in hypothalamic tissue.ResultsWe found that six genes (Sacm1l, Junb, Bmi1, Erbb4, Dkc1, and Suv39h1) are neuron stress-related genes and increased in the HFD-induced mice obesity model, Bmi1gene was identified as a key genes that can reflect the pathophysiology of obesity.ConclusionsOur research depicted a comprehensive activation map of cell abnormality in the obese hypothalamus and Bim1 may be a diagnostic marker in the clinic, which provides a new perspective and basis for investigating the pathogenesis of obesity. |
| format | Article |
| id | doaj-art-c32c960cdbf14b77a6fa5c9b6a92234e |
| institution | Kabale University |
| issn | 1664-2392 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj-art-c32c960cdbf14b77a6fa5c9b6a92234e2024-12-09T04:24:57ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922024-12-011510.3389/fendo.2024.14438801443880Neuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat dietCaixia Liang0Caixia Liang1Caixia Liang2Caixia Liang3Hongjian Lu4Xueqin Wang5Jianbin Su6Feng Qi7Yanxing Shang8Yanxing Shang9Yanxing Shang10Yanxing Shang11Yu Li12Dongmei Zhang13Dongmei Zhang14Dongmei Zhang15Dongmei Zhang16Dongmei Zhang17Chengwei Duan18Chengwei Duan19Chengwei Duan20Chengwei Duan21Medical Research Center, Affiliated Hospital 2 of Nantong University, Nantong, ChinaJiangsu Provincial Medical Key Discipline (Laboratory) Cultivation Unit, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaNantong Municipal Medical Key Laboratory of Molecular Immunology, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaNantong Municipal Key Laboratory of Metabolic Immunology and Disease Microenvironment, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaDepartment of Rehabilitation Medicine, Affiliated Hospital 2 of Nantong University, Nantong, ChinaDepartment of Endocrinology, Affiliated Hospital 2 of Nantong University, Nantong, ChinaDepartment of Endocrinology, Affiliated Hospital 2 of Nantong University, Nantong, ChinaEmergency Intensive Care Unit, Affiliated Hospital 2 of Nantong University and First People’s Hospital of Nantong City, Nantong, ChinaMedical Research Center, Affiliated Hospital 2 of Nantong University, Nantong, ChinaJiangsu Provincial Medical Key Discipline (Laboratory) Cultivation Unit, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaNantong Municipal Medical Key Laboratory of Molecular Immunology, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaNantong Municipal Key Laboratory of Metabolic Immunology and Disease Microenvironment, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaDepartment of Pathogen Biology, Medical College, Nantong University, Nantong, ChinaMedical Research Center, Affiliated Hospital 2 of Nantong University, Nantong, ChinaJiangsu Provincial Medical Key Discipline (Laboratory) Cultivation Unit, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaNantong Municipal Medical Key Laboratory of Molecular Immunology, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaNantong Municipal Key Laboratory of Metabolic Immunology and Disease Microenvironment, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaDepartment of Pathogen Biology, Medical College, Nantong University, Nantong, ChinaMedical Research Center, Affiliated Hospital 2 of Nantong University, Nantong, ChinaJiangsu Provincial Medical Key Discipline (Laboratory) Cultivation Unit, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaNantong Municipal Medical Key Laboratory of Molecular Immunology, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaNantong Municipal Key Laboratory of Metabolic Immunology and Disease Microenvironment, Medical Research Center, Nantong First People’s Hospital, Nantong, ChinaObjectiveEnergy homeostasis is modulated by the hypothalamic is essential for obesity progression, however, the gene expression profiling remains to be fully understood.MethodsGEO datasets were downloaded from the GEO website and analyzed by the R packages to obtain the DEGs. And, the WGCNA analysis and PPI networks of co-expressed DEGs were designed using STRING to get key genes. In addition, the single-cell sequencing datasets and GTEx database were utilized to receive the neuron-stress genes from the key genes. Further, high-fat diet (HFD)-induced hypothalamic tissue of mice was used as an animal model to validate the mRNA up-regulation of neuron-stress genes. In addition, the Bmi1 gene was identified as a hub gene through the LASSO model and nomogram analysis. Western blot confirmed the high expression of Bmi1 in hypothalamic tissue of HFD mice and PA-stimulated microglia. Immunofluorescence staining showed that HFD induced the activation of microglia and the expression of Bmi1 in hypothalamic tissue.ResultsWe found that six genes (Sacm1l, Junb, Bmi1, Erbb4, Dkc1, and Suv39h1) are neuron stress-related genes and increased in the HFD-induced mice obesity model, Bmi1gene was identified as a key genes that can reflect the pathophysiology of obesity.ConclusionsOur research depicted a comprehensive activation map of cell abnormality in the obese hypothalamus and Bim1 may be a diagnostic marker in the clinic, which provides a new perspective and basis for investigating the pathogenesis of obesity.https://www.frontiersin.org/articles/10.3389/fendo.2024.1443880/fullobesityneuron stresshypothalamic inflammationBMI1WGCNA |
| spellingShingle | Caixia Liang Caixia Liang Caixia Liang Caixia Liang Hongjian Lu Xueqin Wang Jianbin Su Feng Qi Yanxing Shang Yanxing Shang Yanxing Shang Yanxing Shang Yu Li Dongmei Zhang Dongmei Zhang Dongmei Zhang Dongmei Zhang Dongmei Zhang Chengwei Duan Chengwei Duan Chengwei Duan Chengwei Duan Neuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat diet Frontiers in Endocrinology obesity neuron stress hypothalamic inflammation BMI1 WGCNA |
| title | Neuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat diet |
| title_full | Neuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat diet |
| title_fullStr | Neuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat diet |
| title_full_unstemmed | Neuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat diet |
| title_short | Neuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat diet |
| title_sort | neuron stress related genes serve as new biomarkers in hypothalamic tissue following high fat diet |
| topic | obesity neuron stress hypothalamic inflammation BMI1 WGCNA |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2024.1443880/full |
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