Nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system-mediated proinflammatory disease
Abstract The correlation between circulating microbes and sepsis as well as proinflammatory diseases is increasingly gaining recognition. However, the detection of microbes’ cell-free DNA (cfDNA), which exist at concentrations of a billion times lower than blood proteins, poses a significant challen...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-80126-6 |
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| author | Kessarin Thanapirom Walid Al-Akkad Aylin Pelut Zahra Sadouki Jemima B. Finkel Stefan Nardi-Hiebl Wieland Vogt Benjamin Vojnar Hinnerk Wulf Leopold Eberhart Timothy D McHugh Krista Rombouts Massimo Pinzani Emmanouil Tsochatzis Joseph W. Ndieyira |
| author_facet | Kessarin Thanapirom Walid Al-Akkad Aylin Pelut Zahra Sadouki Jemima B. Finkel Stefan Nardi-Hiebl Wieland Vogt Benjamin Vojnar Hinnerk Wulf Leopold Eberhart Timothy D McHugh Krista Rombouts Massimo Pinzani Emmanouil Tsochatzis Joseph W. Ndieyira |
| author_sort | Kessarin Thanapirom |
| collection | DOAJ |
| description | Abstract The correlation between circulating microbes and sepsis as well as proinflammatory diseases is increasingly gaining recognition. However, the detection of microbes’ cell-free DNA (cfDNA), which exist at concentrations of a billion times lower than blood proteins, poses a significant challenge for early disease detection. Here, we present Nano mechanics combined with highly sensitive readout sequences to address the challenges of ultralow counts of disease biomarkers, thus enabling robust quantitative monitoring of chronic medical conditions at different stages of human disease progression. To showcase the effectiveness of our approach, we employ fragments of cfDNA and human cell secretory proteins as models with predictive capabilities for human diseases. Notably, our method reveals a reliable representation over an impressive three to four orders of magnitude in the detection limit and dynamic range, surpassing commercially available quantitative polymerase chain reaction (qPCR) commonly used in routine clinical practice. This concept underpins a highly sensitive and selective medical device designed for the early detection of circulating microbes in patients undergoing intensive cancer therapy. This will help pinpoint individuals at risk of complications, including damage to the intestinal barrier and development of neutropenic fever/Sirsa/Sepsis. Moreover, this approach introduces new avenues for stratifying antibiotic prophylaxis in proinflammatory diseases. |
| format | Article |
| id | doaj-art-c31cbbd3f65d4a53a33cf63e989bc217 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-c31cbbd3f65d4a53a33cf63e989bc2172024-12-08T12:28:53ZengNature PortfolioScientific Reports2045-23222024-12-0114112010.1038/s41598-024-80126-6Nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system-mediated proinflammatory diseaseKessarin Thanapirom0Walid Al-Akkad1Aylin Pelut2Zahra Sadouki3Jemima B. Finkel4Stefan Nardi-Hiebl5Wieland Vogt6Benjamin Vojnar7Hinnerk Wulf8Leopold Eberhart9Timothy D McHugh10Krista Rombouts11Massimo Pinzani12Emmanouil Tsochatzis13Joseph W. Ndieyira14Division of Medicine, University College LondonDivision of Medicine, University College LondonDivision of Medicine, University College LondonUCL Centre for Clinical Microbiology, Division of Infection and Immunity, University College LondonDivision of Medicine, University College LondonDepartment of Anaesthesia and Intensive Care, University Hospital of the Philipps-University of Marburg BaldingerstrasseMedical Innovations and Management, Steinbeis UniversityDepartment of Anaesthesia and Intensive Care, University Hospital of the Philipps-University of Marburg BaldingerstrasseDepartment of Anaesthesia and Intensive Care, University Hospital of the Philipps-University of Marburg BaldingerstrasseDepartment of Anaesthesia and Intensive Care, University Hospital of the Philipps-University of Marburg BaldingerstrasseUCL Centre for Clinical Microbiology, Division of Infection and Immunity, University College LondonDivision of Medicine, University College LondonDivision of Medicine, University College LondonDivision of Medicine, University College LondonDivision of Medicine, University College LondonAbstract The correlation between circulating microbes and sepsis as well as proinflammatory diseases is increasingly gaining recognition. However, the detection of microbes’ cell-free DNA (cfDNA), which exist at concentrations of a billion times lower than blood proteins, poses a significant challenge for early disease detection. Here, we present Nano mechanics combined with highly sensitive readout sequences to address the challenges of ultralow counts of disease biomarkers, thus enabling robust quantitative monitoring of chronic medical conditions at different stages of human disease progression. To showcase the effectiveness of our approach, we employ fragments of cfDNA and human cell secretory proteins as models with predictive capabilities for human diseases. Notably, our method reveals a reliable representation over an impressive three to four orders of magnitude in the detection limit and dynamic range, surpassing commercially available quantitative polymerase chain reaction (qPCR) commonly used in routine clinical practice. This concept underpins a highly sensitive and selective medical device designed for the early detection of circulating microbes in patients undergoing intensive cancer therapy. This will help pinpoint individuals at risk of complications, including damage to the intestinal barrier and development of neutropenic fever/Sirsa/Sepsis. Moreover, this approach introduces new avenues for stratifying antibiotic prophylaxis in proinflammatory diseases.https://doi.org/10.1038/s41598-024-80126-6 |
| spellingShingle | Kessarin Thanapirom Walid Al-Akkad Aylin Pelut Zahra Sadouki Jemima B. Finkel Stefan Nardi-Hiebl Wieland Vogt Benjamin Vojnar Hinnerk Wulf Leopold Eberhart Timothy D McHugh Krista Rombouts Massimo Pinzani Emmanouil Tsochatzis Joseph W. Ndieyira Nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system-mediated proinflammatory disease Scientific Reports |
| title | Nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system-mediated proinflammatory disease |
| title_full | Nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system-mediated proinflammatory disease |
| title_fullStr | Nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system-mediated proinflammatory disease |
| title_full_unstemmed | Nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system-mediated proinflammatory disease |
| title_short | Nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system-mediated proinflammatory disease |
| title_sort | nanomechanical detection to empower robust monitoring of sepsis and microbial adaptive immune system mediated proinflammatory disease |
| url | https://doi.org/10.1038/s41598-024-80126-6 |
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