Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profiling
Objective Probiotics are living microorganisms that can provide health benefits when consumed. Here, we investigated the effects of probiotics on gene expression in the spleen of mice using RNA-sequencing analysis between negative control and probiotic groups (including 4 Lactobacillus strains: Lact...
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| Format: | Article |
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Asian-Australasian Association of Animal Production Societies
2025-02-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0280.pdf |
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| author | Anh Duc Truong Ha Thi Thanh Tran Nhu Thi Chu Lanh Phan Hoai Thi Phan Thu Huong Dang Hoang Vu Dang La Anh Nguyen |
| author_facet | Anh Duc Truong Ha Thi Thanh Tran Nhu Thi Chu Lanh Phan Hoai Thi Phan Thu Huong Dang Hoang Vu Dang La Anh Nguyen |
| author_sort | Anh Duc Truong |
| collection | DOAJ |
| description | Objective Probiotics are living microorganisms that can provide health benefits when consumed. Here, we investigated the effects of probiotics on gene expression in the spleen of mice using RNA-sequencing analysis between negative control and probiotic groups (including 4 Lactobacillus strains: Lactobacillus fermentum, L. casei, L. plantarum, and L. brevis). Methods Mice exposed with probiotic in 4 weeks by intragastric administration. Then, spleen tissues of the control and probiotics groups were collected on days 14 and 28 for RNA sequencing. Results In total, 665, 186, and 81 differentially expressed genes (DEGs) were significantly expressed on day 14 vs control, day 28 vs control groups, and probiotics day 28 vs day 14 groups, respectively. On the other hand, 12 toll-like receptor genes underwent additional validation through quantitative real-time polymerase chain reaction (qRT-PCR), affirming the increased alignment between qRT-PCR and RNA-Seq findings. In addition, the Kyoto encyclopedia of genes and genomes and gene ontology analyses revealed that the DEGs were predominantly enriched in defense responses to pathogens, including inflammatory bowel diseases, malaria, leukaemia virus 1, and herpes virus, as well as immune processes related to immune response and signal transduction. This study represents the first investigation into mice’s gene expression in the spleen exposed to probiotics using Lactobacillus spp. isolated from a field strain in Vietnam. Conclusion Our results provide valuable insights into the impacts and functions of probiotics on mammalian development, offering crucial information for the potential therapeutic use of probiotics in defending against pathogens in Vietnam. The findings from this study highlight the potential of probiotics in modulating gene expression in the spleen, which may have implications for immune function and overall health in mice. |
| format | Article |
| id | doaj-art-c2fb1de470254d98921c00110dd4e456 |
| institution | Kabale University |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-c2fb1de470254d98921c00110dd4e4562025-01-03T04:14:22ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-02-0138233634910.5713/ab.24.028025324Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profilingAnh Duc Truong0Ha Thi Thanh Tran1Nhu Thi Chu2Lanh Phan3Hoai Thi Phan4Thu Huong Dang5Hoang Vu Dang6La Anh Nguyen7 Department of Biochemistry and Immunology, National Institute of Veterinary Research, Dong Da, Hanoi 100000, Vietnam Department of Biochemistry and Immunology, National Institute of Veterinary Research, Dong Da, Hanoi 100000, Vietnam Department of Biochemistry and Immunology, National Institute of Veterinary Research, Dong Da, Hanoi 100000, Vietnam Department of Biochemistry and Immunology, National Institute of Veterinary Research, Dong Da, Hanoi 100000, Vietnam Department of Biochemistry and Immunology, National Institute of Veterinary Research, Dong Da, Hanoi 100000, Vietnam Department of Microbial Biotechnology, Food Industries Research Institute, Thanh Xuan Distr., Hanoi 100000, Vietnam Department of Microbial Biotechnology, Food Industries Research Institute, Thanh Xuan Distr., Hanoi 100000, Vietnam Department of Microbial Biotechnology, Food Industries Research Institute, Thanh Xuan Distr., Hanoi 100000, VietnamObjective Probiotics are living microorganisms that can provide health benefits when consumed. Here, we investigated the effects of probiotics on gene expression in the spleen of mice using RNA-sequencing analysis between negative control and probiotic groups (including 4 Lactobacillus strains: Lactobacillus fermentum, L. casei, L. plantarum, and L. brevis). Methods Mice exposed with probiotic in 4 weeks by intragastric administration. Then, spleen tissues of the control and probiotics groups were collected on days 14 and 28 for RNA sequencing. Results In total, 665, 186, and 81 differentially expressed genes (DEGs) were significantly expressed on day 14 vs control, day 28 vs control groups, and probiotics day 28 vs day 14 groups, respectively. On the other hand, 12 toll-like receptor genes underwent additional validation through quantitative real-time polymerase chain reaction (qRT-PCR), affirming the increased alignment between qRT-PCR and RNA-Seq findings. In addition, the Kyoto encyclopedia of genes and genomes and gene ontology analyses revealed that the DEGs were predominantly enriched in defense responses to pathogens, including inflammatory bowel diseases, malaria, leukaemia virus 1, and herpes virus, as well as immune processes related to immune response and signal transduction. This study represents the first investigation into mice’s gene expression in the spleen exposed to probiotics using Lactobacillus spp. isolated from a field strain in Vietnam. Conclusion Our results provide valuable insights into the impacts and functions of probiotics on mammalian development, offering crucial information for the potential therapeutic use of probiotics in defending against pathogens in Vietnam. The findings from this study highlight the potential of probiotics in modulating gene expression in the spleen, which may have implications for immune function and overall health in mice.http://www.animbiosci.org/upload/pdf/ab-24-0280.pdfdifferentially expressed geneimmune response spp.probioticsignaling pathway |
| spellingShingle | Anh Duc Truong Ha Thi Thanh Tran Nhu Thi Chu Lanh Phan Hoai Thi Phan Thu Huong Dang Hoang Vu Dang La Anh Nguyen Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profiling Animal Bioscience differentially expressed gene immune response spp. probiotic signaling pathway |
| title | Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profiling |
| title_full | Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profiling |
| title_fullStr | Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profiling |
| title_full_unstemmed | Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profiling |
| title_short | Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profiling |
| title_sort | identification of immune associated genes with altered expression in the spleen of mice enriched with probiotic species using rna seq profiling |
| topic | differentially expressed gene immune response spp. probiotic signaling pathway |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0280.pdf |
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