Translational research from bioinformatics to animal studies: Exploring gene expression and muscle health in COPD through selenium nanoparticles and exercise
Recent studies highlight the role of molecular pathways, such as oxidative stress response and mitochondrial function, in COPD. This study explores the role of the PGC-1α gene, a key regulator of mitochondrial biogenesis and energy metabolism, using a rat model and bioinformatics analysis of human l...
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| Format: | Article |
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2024-06-01
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| Series: | Journal of Exercise & Organ Cross Talk |
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| Online Access: | https://www.jeoct.com/article_209436_2303630e362c062f831362f1469511c4.pdf |
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| author | Mahdi Bakhshi Abdolali Bnaeifar Sajjad Arshadi Behzad Bazgir |
| author_facet | Mahdi Bakhshi Abdolali Bnaeifar Sajjad Arshadi Behzad Bazgir |
| author_sort | Mahdi Bakhshi |
| collection | DOAJ |
| description | Recent studies highlight the role of molecular pathways, such as oxidative stress response and mitochondrial function, in COPD. This study explores the role of the PGC-1α gene, a key regulator of mitochondrial biogenesis and energy metabolism, using a rat model and bioinformatics analysis of human lung tissue samples. This study utilized a combined approach, analyzing gene expression in rat lung tissue alongside bioinformatics analysis of public human datasets. A total of 42 male Wistar rats were divided into seven groups, receiving treatments including cigarette smoke extract (CSE), nano-selenium (SeNPs), and aerobic interval training (AIT). PGC-1α expression levels were evaluated using quantitative Real-Time PCR (qRT-PCR) and analyzed using one-way ANOVA, followed by Dunnett’s post hoc test for multiple comparisons to determine significance across groups. The CSE+SeNPs+AIT group exhibited significantly higher PGC-1α expression compared to controls (p = 0.0289), indicating a potential protective role of SeNPs and exercise against oxidative stress. Bioinformatics analysis identified 250 differentially expressed genes (DEGs), with PGC-1α emerging as a critical hub gene associated with pathways like oxidative stress response and mitochondrial regulation. Protein-protein interaction (PPI) analysis further highlighted the centrality of PGC-1α in COPD pathophysiology. This study underscores the importance of PGC-1α in regulating mitochondrial function and oxidative stress in COPD. The findings suggest that PGC-1α could serve as a potential therapeutic target, offering insights into the development of interventions aimed at improving respiratory health in COPD patients. Future research should focus on validating these findings in clinical settings and exploring the therapeutic potential of PGC-1α modulation. |
| format | Article |
| id | doaj-art-c2cf2dd47fc743cdab6bb802f6710aef |
| institution | Kabale University |
| issn | 2783-2074 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | JEOCT publisher |
| record_format | Article |
| series | Journal of Exercise & Organ Cross Talk |
| spelling | doaj-art-c2cf2dd47fc743cdab6bb802f6710aef2024-12-17T19:30:35ZengJEOCT publisherJournal of Exercise & Organ Cross Talk2783-20742024-06-014210711610.22122/jeoct.2024.485319.1129209436Translational research from bioinformatics to animal studies: Exploring gene expression and muscle health in COPD through selenium nanoparticles and exerciseMahdi Bakhshi0Abdolali Bnaeifar1Sajjad Arshadi2Behzad Bazgir3Department of Physical Education and Sports Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran.Department of Physical Education and Sports Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran.Department of Physical Education and Sports Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran.Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences. Tehran, Iran.Recent studies highlight the role of molecular pathways, such as oxidative stress response and mitochondrial function, in COPD. This study explores the role of the PGC-1α gene, a key regulator of mitochondrial biogenesis and energy metabolism, using a rat model and bioinformatics analysis of human lung tissue samples. This study utilized a combined approach, analyzing gene expression in rat lung tissue alongside bioinformatics analysis of public human datasets. A total of 42 male Wistar rats were divided into seven groups, receiving treatments including cigarette smoke extract (CSE), nano-selenium (SeNPs), and aerobic interval training (AIT). PGC-1α expression levels were evaluated using quantitative Real-Time PCR (qRT-PCR) and analyzed using one-way ANOVA, followed by Dunnett’s post hoc test for multiple comparisons to determine significance across groups. The CSE+SeNPs+AIT group exhibited significantly higher PGC-1α expression compared to controls (p = 0.0289), indicating a potential protective role of SeNPs and exercise against oxidative stress. Bioinformatics analysis identified 250 differentially expressed genes (DEGs), with PGC-1α emerging as a critical hub gene associated with pathways like oxidative stress response and mitochondrial regulation. Protein-protein interaction (PPI) analysis further highlighted the centrality of PGC-1α in COPD pathophysiology. This study underscores the importance of PGC-1α in regulating mitochondrial function and oxidative stress in COPD. The findings suggest that PGC-1α could serve as a potential therapeutic target, offering insights into the development of interventions aimed at improving respiratory health in COPD patients. Future research should focus on validating these findings in clinical settings and exploring the therapeutic potential of PGC-1α modulation.https://www.jeoct.com/article_209436_2303630e362c062f831362f1469511c4.pdfpgc-1αcopdgene expressionoxidative stressbioinformatics |
| spellingShingle | Mahdi Bakhshi Abdolali Bnaeifar Sajjad Arshadi Behzad Bazgir Translational research from bioinformatics to animal studies: Exploring gene expression and muscle health in COPD through selenium nanoparticles and exercise Journal of Exercise & Organ Cross Talk pgc-1α copd gene expression oxidative stress bioinformatics |
| title | Translational research from bioinformatics to animal studies: Exploring gene expression and muscle health in COPD through selenium nanoparticles and exercise |
| title_full | Translational research from bioinformatics to animal studies: Exploring gene expression and muscle health in COPD through selenium nanoparticles and exercise |
| title_fullStr | Translational research from bioinformatics to animal studies: Exploring gene expression and muscle health in COPD through selenium nanoparticles and exercise |
| title_full_unstemmed | Translational research from bioinformatics to animal studies: Exploring gene expression and muscle health in COPD through selenium nanoparticles and exercise |
| title_short | Translational research from bioinformatics to animal studies: Exploring gene expression and muscle health in COPD through selenium nanoparticles and exercise |
| title_sort | translational research from bioinformatics to animal studies exploring gene expression and muscle health in copd through selenium nanoparticles and exercise |
| topic | pgc-1α copd gene expression oxidative stress bioinformatics |
| url | https://www.jeoct.com/article_209436_2303630e362c062f831362f1469511c4.pdf |
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