Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerance
Abiotic stress is the major bottleneck in obtaining the full potential of a standing crop. Various strategies have been investigated to impart abiotic stress tolerance. In the agricultural field, the stress conditions do not act in isolation rather they act synergistically in combinations against th...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364724004877 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846140799710920704 |
|---|---|
| author | Pamela Chanda Roy Deepak Kumar Ojha Welka Sahu Mohammad Khalid Al-Sadoon K. Sony Reddy Gopal Chowdhary |
| author_facet | Pamela Chanda Roy Deepak Kumar Ojha Welka Sahu Mohammad Khalid Al-Sadoon K. Sony Reddy Gopal Chowdhary |
| author_sort | Pamela Chanda Roy |
| collection | DOAJ |
| description | Abiotic stress is the major bottleneck in obtaining the full potential of a standing crop. Various strategies have been investigated to impart abiotic stress tolerance. In the agricultural field, the stress conditions do not act in isolation rather they act synergistically in combinations against the wellbeing of the crop. The combined impact of abiotic stress conditions has been referred to as stress matrix or stress combination. Here, we have proposed a probable candidate that could be instrumental in imparting tolerance against stress matrix or stress combination. The investigation was made on a peroxisome localized glutathione reductase (GR) enzyme from the economically significant crop Oryza sativa. The protein was expressed in the E. coli system, purified and characterized. The enzyme was found to be active even at an elevated temperature of 55℃, demonstrating its abiotic stress tolerance properties. Further, the gene expression studies showed it to be upregulated under salinity, dehydration, low and high temperature. The expression was also investigated under various stress combinations and it was observed that the level of GR mRNA was found to be higher in the case of combined stress treatment as compared to individual stress conditions suggesting an additive effect phenomenon. The results obtained here put this peroxisomal GR in the spotlight for imparting abiotic stress combinations studies. |
| format | Article |
| id | doaj-art-c2cae09c50954de29ba9e07f6f380d49 |
| institution | Kabale University |
| issn | 1018-3647 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj-art-c2cae09c50954de29ba9e07f6f380d492024-12-05T05:19:29ZengElsevierJournal of King Saud University: Science1018-36472024-12-013611103575Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerancePamela Chanda Roy0Deepak Kumar Ojha1Welka Sahu2Mohammad Khalid Al-Sadoon3K. Sony Reddy4Gopal Chowdhary5Plant Molecular Biology Laboratory, School of Biotechnology, KIIT University, Bhubaneswar 751024, Odisha, IndiaImmunology Laboratory, School of Biotechnology, KIIT University, Bhubaneswar 751024, Odisha, IndiaImmunology Laboratory, School of Biotechnology, KIIT University, Bhubaneswar 751024, Odisha, IndiaDepartment of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi ArabiaImmunology Laboratory, School of Biotechnology, KIIT University, Bhubaneswar 751024, Odisha, IndiaPlant Molecular Biology Laboratory, School of Biotechnology, KIIT University, Bhubaneswar 751024, Odisha, India; Corresponding authors.Abiotic stress is the major bottleneck in obtaining the full potential of a standing crop. Various strategies have been investigated to impart abiotic stress tolerance. In the agricultural field, the stress conditions do not act in isolation rather they act synergistically in combinations against the wellbeing of the crop. The combined impact of abiotic stress conditions has been referred to as stress matrix or stress combination. Here, we have proposed a probable candidate that could be instrumental in imparting tolerance against stress matrix or stress combination. The investigation was made on a peroxisome localized glutathione reductase (GR) enzyme from the economically significant crop Oryza sativa. The protein was expressed in the E. coli system, purified and characterized. The enzyme was found to be active even at an elevated temperature of 55℃, demonstrating its abiotic stress tolerance properties. Further, the gene expression studies showed it to be upregulated under salinity, dehydration, low and high temperature. The expression was also investigated under various stress combinations and it was observed that the level of GR mRNA was found to be higher in the case of combined stress treatment as compared to individual stress conditions suggesting an additive effect phenomenon. The results obtained here put this peroxisomal GR in the spotlight for imparting abiotic stress combinations studies.http://www.sciencedirect.com/science/article/pii/S1018364724004877PeroxisomeGlutathione reductaseStress combinationHeat stable |
| spellingShingle | Pamela Chanda Roy Deepak Kumar Ojha Welka Sahu Mohammad Khalid Al-Sadoon K. Sony Reddy Gopal Chowdhary Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerance Journal of King Saud University: Science Peroxisome Glutathione reductase Stress combination Heat stable |
| title | Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerance |
| title_full | Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerance |
| title_fullStr | Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerance |
| title_full_unstemmed | Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerance |
| title_short | Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerance |
| title_sort | overexpression purification and characterization of a heat stable peroxisomal glutathione reductase from oryza sativa involved in multifactorial abiotic stress tolerance |
| topic | Peroxisome Glutathione reductase Stress combination Heat stable |
| url | http://www.sciencedirect.com/science/article/pii/S1018364724004877 |
| work_keys_str_mv | AT pamelachandaroy overexpressionpurificationandcharacterizationofaheatstableperoxisomalglutathionereductasefromoryzasativainvolvedinmultifactorialabioticstresstolerance AT deepakkumarojha overexpressionpurificationandcharacterizationofaheatstableperoxisomalglutathionereductasefromoryzasativainvolvedinmultifactorialabioticstresstolerance AT welkasahu overexpressionpurificationandcharacterizationofaheatstableperoxisomalglutathionereductasefromoryzasativainvolvedinmultifactorialabioticstresstolerance AT mohammadkhalidalsadoon overexpressionpurificationandcharacterizationofaheatstableperoxisomalglutathionereductasefromoryzasativainvolvedinmultifactorialabioticstresstolerance AT ksonyreddy overexpressionpurificationandcharacterizationofaheatstableperoxisomalglutathionereductasefromoryzasativainvolvedinmultifactorialabioticstresstolerance AT gopalchowdhary overexpressionpurificationandcharacterizationofaheatstableperoxisomalglutathionereductasefromoryzasativainvolvedinmultifactorialabioticstresstolerance |