Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae
Bacterial canker is a devastating disease in kiwifruit production, primarily caused by Pseudomonas syringae pv. actinidiae. In this study, a strain of Bacillus velezensis named JIN4, isolated from a kiwifruit branch, showed antagonistic activity. The B. velezensis JIN4 strain was identified based on...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1513438/full |
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| author | Xin Zhao Yang Zhai Lin Wei Fei Xia Yuanru Yang Yongjian Yi Hongying Wang Caisheng Qiu Feng Wang Liangbin Zeng |
| author_facet | Xin Zhao Yang Zhai Lin Wei Fei Xia Yuanru Yang Yongjian Yi Hongying Wang Caisheng Qiu Feng Wang Liangbin Zeng |
| author_sort | Xin Zhao |
| collection | DOAJ |
| description | Bacterial canker is a devastating disease in kiwifruit production, primarily caused by Pseudomonas syringae pv. actinidiae. In this study, a strain of Bacillus velezensis named JIN4, isolated from a kiwifruit branch, showed antagonistic activity. The B. velezensis JIN4 strain was identified based on its morphological, physiological and biochemical characteristics, 16S rDNA, and gyrA sequences. Furthermore, the complete genome of the strain was elucidated, revealing the presence of three genes that confer resistance to antibiotics, including tetracycline, lincomycin, and streptomycin. Additionally, a cluster of nine secondary metabolite synthesis genes was identified. In the laboratory, the JIN4 strain exhibited nitrogen (N) and phosphorus (P) production, demonstrating plant growth-promoting and broad-spectrum inhibitory activities against pathogenic fungi and bacteria. In the field, the JIN4 strain demonstrated effective colonization of kiwifruit, increased antioxidant enzyme activity, induced resistance in kiwifruit plants, and achieved a control efficiency of 60.22% against kiwifruit bacterial canker. These results indicate that B. velezensis JIN4 is a potential biocontrol agent against kiwifruit bacterial canker. |
| format | Article |
| id | doaj-art-c25c6aed04d94db5a4eb0fe5ac702b54 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-c25c6aed04d94db5a4eb0fe5ac702b542024-12-23T06:39:15ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.15134381513438Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiaeXin Zhao0Yang Zhai1Lin Wei2Fei Xia3Yuanru Yang4Yongjian Yi5Hongying Wang6Caisheng Qiu7Feng Wang8Liangbin Zeng9Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaInstitute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, ChinaCollege of Computer and Mathematics, Central South University of Forestry and Technology, Changsha, Hunan, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaGuizhou Academy of Tobacco Science, Guizhou, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaBacterial canker is a devastating disease in kiwifruit production, primarily caused by Pseudomonas syringae pv. actinidiae. In this study, a strain of Bacillus velezensis named JIN4, isolated from a kiwifruit branch, showed antagonistic activity. The B. velezensis JIN4 strain was identified based on its morphological, physiological and biochemical characteristics, 16S rDNA, and gyrA sequences. Furthermore, the complete genome of the strain was elucidated, revealing the presence of three genes that confer resistance to antibiotics, including tetracycline, lincomycin, and streptomycin. Additionally, a cluster of nine secondary metabolite synthesis genes was identified. In the laboratory, the JIN4 strain exhibited nitrogen (N) and phosphorus (P) production, demonstrating plant growth-promoting and broad-spectrum inhibitory activities against pathogenic fungi and bacteria. In the field, the JIN4 strain demonstrated effective colonization of kiwifruit, increased antioxidant enzyme activity, induced resistance in kiwifruit plants, and achieved a control efficiency of 60.22% against kiwifruit bacterial canker. These results indicate that B. velezensis JIN4 is a potential biocontrol agent against kiwifruit bacterial canker.https://www.frontiersin.org/articles/10.3389/fpls.2024.1513438/fullantibacterial activityBacillus velezensisbiocontrol agentkiwifruit bacterial cankerwhole genome sequencing |
| spellingShingle | Xin Zhao Yang Zhai Lin Wei Fei Xia Yuanru Yang Yongjian Yi Hongying Wang Caisheng Qiu Feng Wang Liangbin Zeng Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae Frontiers in Plant Science antibacterial activity Bacillus velezensis biocontrol agent kiwifruit bacterial canker whole genome sequencing |
| title | Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae |
| title_full | Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae |
| title_fullStr | Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae |
| title_full_unstemmed | Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae |
| title_short | Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae |
| title_sort | isolation and identification of a novel bacillus velezensis strain jin4 and its potential for biocontrol of kiwifruit bacterial canker caused by pseudomonas syringae pv actinidiae |
| topic | antibacterial activity Bacillus velezensis biocontrol agent kiwifruit bacterial canker whole genome sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1513438/full |
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