Binding patterns of glycine receptor autoantibodies are related to clinical syndromes
Abstract Patients diagnosed with the rare autoimmune disease Stiff Person Syndrome (SPS) or the more severe form Progressive Encephalomyelitis with Rigidity and Myoclonus (PERM) as well as patients with encephalitis or epilepsy may harbor autoantibodies against synaptic molecules, for example the gl...
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BMC
2025-07-01
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| Series: | Acta Neuropathologica Communications |
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| Online Access: | https://doi.org/10.1186/s40478-025-02082-0 |
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| author | Inken Piro Anna-Lena Wiessler Eleni Kakavela Betül Baykan Erdem Tüzün Carmen Villmann Claudia Sommer |
| author_facet | Inken Piro Anna-Lena Wiessler Eleni Kakavela Betül Baykan Erdem Tüzün Carmen Villmann Claudia Sommer |
| author_sort | Inken Piro |
| collection | DOAJ |
| description | Abstract Patients diagnosed with the rare autoimmune disease Stiff Person Syndrome (SPS) or the more severe form Progressive Encephalomyelitis with Rigidity and Myoclonus (PERM) as well as patients with encephalitis or epilepsy may harbor autoantibodies against synaptic molecules, for example the glycine receptor (GlyR). These autoantibodies interfere with inhibitory signal transmission, which causes a variety of symptoms. How the underlying autoantibody associated pathomechanisms contribute to the variability of clinical presentations, is so far not understood. In this study, binding patterns of GlyR autoantibodies from patients with SPS, PERM and epilepsy to murine central nervous system (CNS) tissue samples were analyzed for disease- and patient-specificity patterns. Twelve GlyR autoantibody positive patients were grouped by the patients’ primary diagnoses. Serum samples from these SPS, PERM and epilepsy patients were evaluated for autoantibody binding on transfected HEK-293 cells and murine spinal cord and various brain tissue samples. Autoantibody binding was further verified by co-localization with commercial antibodies binding to the GlyR and the synaptic marker synaptophysin. Immunochemistry revealed GlyRα1-specific autoantibody binding for all included patients on transfected HEK-293 cells and in the grey matter of murine spinal cord sections. Other CNS regions of enhanced autoantibody accumulation however varied among the groups of SPS, PERM and epilepsy patients and also within groups. Similarly, autoantibody deposits were detected in GlyR expressing higher brain areas for each patient. Even if variations between labeled areas and cell layers were rather patient-specific than group-specific, functionality of the labeled areas aligned with the patients’ impaired functions. Labeled areas and cell layers differing between patients could thereby explain the variability of symptomatology between and within the diseases. The observed diversity suggests a necessity for a personalized approach correlating patient-specific autoantibody properties, phenotype and treatment approaches. |
| format | Article |
| id | doaj-art-c1fad4d07c0545afba6c20e5c101b4a6 |
| institution | Kabale University |
| issn | 2051-5960 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Neuropathologica Communications |
| spelling | doaj-art-c1fad4d07c0545afba6c20e5c101b4a62025-08-20T03:46:12ZengBMCActa Neuropathologica Communications2051-59602025-07-0113111610.1186/s40478-025-02082-0Binding patterns of glycine receptor autoantibodies are related to clinical syndromesInken Piro0Anna-Lena Wiessler1Eleni Kakavela2Betül Baykan3Erdem Tüzün4Carmen Villmann5Claudia Sommer6Department of Neurology, University Hospital WürzburgInstitute for Clinical Neurobiology, University of WürzburgDepartment of Neurology, University Hospital WürzburgDepartment of Neurology and Clinical Neurophysiology, EMAR Medical CenterDepartment of Neuroscience, Institute of Experimental Medicine, Istanbul UniversityInstitute for Clinical Neurobiology, University of WürzburgDepartment of Neurology, University Hospital WürzburgAbstract Patients diagnosed with the rare autoimmune disease Stiff Person Syndrome (SPS) or the more severe form Progressive Encephalomyelitis with Rigidity and Myoclonus (PERM) as well as patients with encephalitis or epilepsy may harbor autoantibodies against synaptic molecules, for example the glycine receptor (GlyR). These autoantibodies interfere with inhibitory signal transmission, which causes a variety of symptoms. How the underlying autoantibody associated pathomechanisms contribute to the variability of clinical presentations, is so far not understood. In this study, binding patterns of GlyR autoantibodies from patients with SPS, PERM and epilepsy to murine central nervous system (CNS) tissue samples were analyzed for disease- and patient-specificity patterns. Twelve GlyR autoantibody positive patients were grouped by the patients’ primary diagnoses. Serum samples from these SPS, PERM and epilepsy patients were evaluated for autoantibody binding on transfected HEK-293 cells and murine spinal cord and various brain tissue samples. Autoantibody binding was further verified by co-localization with commercial antibodies binding to the GlyR and the synaptic marker synaptophysin. Immunochemistry revealed GlyRα1-specific autoantibody binding for all included patients on transfected HEK-293 cells and in the grey matter of murine spinal cord sections. Other CNS regions of enhanced autoantibody accumulation however varied among the groups of SPS, PERM and epilepsy patients and also within groups. Similarly, autoantibody deposits were detected in GlyR expressing higher brain areas for each patient. Even if variations between labeled areas and cell layers were rather patient-specific than group-specific, functionality of the labeled areas aligned with the patients’ impaired functions. Labeled areas and cell layers differing between patients could thereby explain the variability of symptomatology between and within the diseases. The observed diversity suggests a necessity for a personalized approach correlating patient-specific autoantibody properties, phenotype and treatment approaches.https://doi.org/10.1186/s40478-025-02082-0Glycine receptorAutoantibodiesStiff person syndromeBinding pattern |
| spellingShingle | Inken Piro Anna-Lena Wiessler Eleni Kakavela Betül Baykan Erdem Tüzün Carmen Villmann Claudia Sommer Binding patterns of glycine receptor autoantibodies are related to clinical syndromes Acta Neuropathologica Communications Glycine receptor Autoantibodies Stiff person syndrome Binding pattern |
| title | Binding patterns of glycine receptor autoantibodies are related to clinical syndromes |
| title_full | Binding patterns of glycine receptor autoantibodies are related to clinical syndromes |
| title_fullStr | Binding patterns of glycine receptor autoantibodies are related to clinical syndromes |
| title_full_unstemmed | Binding patterns of glycine receptor autoantibodies are related to clinical syndromes |
| title_short | Binding patterns of glycine receptor autoantibodies are related to clinical syndromes |
| title_sort | binding patterns of glycine receptor autoantibodies are related to clinical syndromes |
| topic | Glycine receptor Autoantibodies Stiff person syndrome Binding pattern |
| url | https://doi.org/10.1186/s40478-025-02082-0 |
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