Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis

Abstract We present an advanced electrochemical immunosensor designed to detect the vascular endothelial growth factor (VEGF) precisely. The sensor is constructed on a modified porous gold electrode through a fabrication process involving the deposition of silver and gold on an FTO substrate. Employ...

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Main Authors: Sadaf Yarjoo, Hossein Siampour, Mehrsa Khalilipour, Reza H. Sajedi, Hassan Bagheri, Ahmad Moshaii
Format: Article
Language:English
Published: Nature Portfolio 2024-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-60447-2
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author Sadaf Yarjoo
Hossein Siampour
Mehrsa Khalilipour
Reza H. Sajedi
Hassan Bagheri
Ahmad Moshaii
author_facet Sadaf Yarjoo
Hossein Siampour
Mehrsa Khalilipour
Reza H. Sajedi
Hassan Bagheri
Ahmad Moshaii
author_sort Sadaf Yarjoo
collection DOAJ
description Abstract We present an advanced electrochemical immunosensor designed to detect the vascular endothelial growth factor (VEGF) precisely. The sensor is constructed on a modified porous gold electrode through a fabrication process involving the deposition of silver and gold on an FTO substrate. Employing thermal annealing and a de-alloying process, the silver is eliminated from the electrode, producing a reproducible porous gold substrate. Utilizing a well-defined protocol, we immobilize the heavy-chain (VHH) antibody against VEGF on the gold substrate, facilitating VEGF detection through various electrochemical methods. Remarkably, this immunosensor performs well, featuring an impressive detection limit of 0.05 pg/mL and an extensive linear range from 0.1 pg/mL to 0.1 µg/mL. This emphasizes it’s to measure biomarkers across a wide concentration spectrum precisely. The robust fabrication methodology in this research underscores its potential for widespread application, offering enhanced precision, reproducibility, and remarkable detection capabilities for the developed immunosensor.
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institution Kabale University
issn 2045-2322
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publishDate 2024-05-01
publisher Nature Portfolio
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series Scientific Reports
spelling doaj-art-c1e6b33a2bf04fdf8cdb66b767ad7b432024-12-01T12:20:36ZengNature PortfolioScientific Reports2045-23222024-05-0114111110.1038/s41598-024-60447-2Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosisSadaf Yarjoo0Hossein Siampour1Mehrsa Khalilipour2Reza H. Sajedi3Hassan Bagheri4Ahmad Moshaii5Department of Physics, Tarbiat Modares UniversityDepartment of Physics, Tarbiat Modares UniversityDepartment of Physics, Tarbiat Modares UniversityDepartment of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Jalal Ale Ahmad HighwayChemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical SciencesDepartment of Physics, Tarbiat Modares UniversityAbstract We present an advanced electrochemical immunosensor designed to detect the vascular endothelial growth factor (VEGF) precisely. The sensor is constructed on a modified porous gold electrode through a fabrication process involving the deposition of silver and gold on an FTO substrate. Employing thermal annealing and a de-alloying process, the silver is eliminated from the electrode, producing a reproducible porous gold substrate. Utilizing a well-defined protocol, we immobilize the heavy-chain (VHH) antibody against VEGF on the gold substrate, facilitating VEGF detection through various electrochemical methods. Remarkably, this immunosensor performs well, featuring an impressive detection limit of 0.05 pg/mL and an extensive linear range from 0.1 pg/mL to 0.1 µg/mL. This emphasizes it’s to measure biomarkers across a wide concentration spectrum precisely. The robust fabrication methodology in this research underscores its potential for widespread application, offering enhanced precision, reproducibility, and remarkable detection capabilities for the developed immunosensor.https://doi.org/10.1038/s41598-024-60447-2Electrochemical immunosensorVEGF detectionPorous gold electrodeFabrication methodologyBiomarker measurement
spellingShingle Sadaf Yarjoo
Hossein Siampour
Mehrsa Khalilipour
Reza H. Sajedi
Hassan Bagheri
Ahmad Moshaii
Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis
Scientific Reports
Electrochemical immunosensor
VEGF detection
Porous gold electrode
Fabrication methodology
Biomarker measurement
title Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis
title_full Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis
title_fullStr Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis
title_full_unstemmed Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis
title_short Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis
title_sort gold nanostructure enhanced immunosensing ultra sensitive detection of vegf tumor marker for early disease diagnosis
topic Electrochemical immunosensor
VEGF detection
Porous gold electrode
Fabrication methodology
Biomarker measurement
url https://doi.org/10.1038/s41598-024-60447-2
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