Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis
Abstract We present an advanced electrochemical immunosensor designed to detect the vascular endothelial growth factor (VEGF) precisely. The sensor is constructed on a modified porous gold electrode through a fabrication process involving the deposition of silver and gold on an FTO substrate. Employ...
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Nature Portfolio
2024-05-01
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Online Access: | https://doi.org/10.1038/s41598-024-60447-2 |
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author | Sadaf Yarjoo Hossein Siampour Mehrsa Khalilipour Reza H. Sajedi Hassan Bagheri Ahmad Moshaii |
author_facet | Sadaf Yarjoo Hossein Siampour Mehrsa Khalilipour Reza H. Sajedi Hassan Bagheri Ahmad Moshaii |
author_sort | Sadaf Yarjoo |
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description | Abstract We present an advanced electrochemical immunosensor designed to detect the vascular endothelial growth factor (VEGF) precisely. The sensor is constructed on a modified porous gold electrode through a fabrication process involving the deposition of silver and gold on an FTO substrate. Employing thermal annealing and a de-alloying process, the silver is eliminated from the electrode, producing a reproducible porous gold substrate. Utilizing a well-defined protocol, we immobilize the heavy-chain (VHH) antibody against VEGF on the gold substrate, facilitating VEGF detection through various electrochemical methods. Remarkably, this immunosensor performs well, featuring an impressive detection limit of 0.05 pg/mL and an extensive linear range from 0.1 pg/mL to 0.1 µg/mL. This emphasizes it’s to measure biomarkers across a wide concentration spectrum precisely. The robust fabrication methodology in this research underscores its potential for widespread application, offering enhanced precision, reproducibility, and remarkable detection capabilities for the developed immunosensor. |
format | Article |
id | doaj-art-c1e6b33a2bf04fdf8cdb66b767ad7b43 |
institution | Kabale University |
issn | 2045-2322 |
language | English |
publishDate | 2024-05-01 |
publisher | Nature Portfolio |
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series | Scientific Reports |
spelling | doaj-art-c1e6b33a2bf04fdf8cdb66b767ad7b432024-12-01T12:20:36ZengNature PortfolioScientific Reports2045-23222024-05-0114111110.1038/s41598-024-60447-2Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosisSadaf Yarjoo0Hossein Siampour1Mehrsa Khalilipour2Reza H. Sajedi3Hassan Bagheri4Ahmad Moshaii5Department of Physics, Tarbiat Modares UniversityDepartment of Physics, Tarbiat Modares UniversityDepartment of Physics, Tarbiat Modares UniversityDepartment of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Jalal Ale Ahmad HighwayChemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical SciencesDepartment of Physics, Tarbiat Modares UniversityAbstract We present an advanced electrochemical immunosensor designed to detect the vascular endothelial growth factor (VEGF) precisely. The sensor is constructed on a modified porous gold electrode through a fabrication process involving the deposition of silver and gold on an FTO substrate. Employing thermal annealing and a de-alloying process, the silver is eliminated from the electrode, producing a reproducible porous gold substrate. Utilizing a well-defined protocol, we immobilize the heavy-chain (VHH) antibody against VEGF on the gold substrate, facilitating VEGF detection through various electrochemical methods. Remarkably, this immunosensor performs well, featuring an impressive detection limit of 0.05 pg/mL and an extensive linear range from 0.1 pg/mL to 0.1 µg/mL. This emphasizes it’s to measure biomarkers across a wide concentration spectrum precisely. The robust fabrication methodology in this research underscores its potential for widespread application, offering enhanced precision, reproducibility, and remarkable detection capabilities for the developed immunosensor.https://doi.org/10.1038/s41598-024-60447-2Electrochemical immunosensorVEGF detectionPorous gold electrodeFabrication methodologyBiomarker measurement |
spellingShingle | Sadaf Yarjoo Hossein Siampour Mehrsa Khalilipour Reza H. Sajedi Hassan Bagheri Ahmad Moshaii Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis Scientific Reports Electrochemical immunosensor VEGF detection Porous gold electrode Fabrication methodology Biomarker measurement |
title | Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis |
title_full | Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis |
title_fullStr | Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis |
title_full_unstemmed | Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis |
title_short | Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis |
title_sort | gold nanostructure enhanced immunosensing ultra sensitive detection of vegf tumor marker for early disease diagnosis |
topic | Electrochemical immunosensor VEGF detection Porous gold electrode Fabrication methodology Biomarker measurement |
url | https://doi.org/10.1038/s41598-024-60447-2 |
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