Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genes
Stripe rust (Puccinia striiformis f. sp. tritici) poses a major threat to Chinese wheat production. Assessing resistance levels and gene distribution in spring wheat landraces is critical for breeding durable resistant cultivars. This study evaluated 267 landraces from eight provinces for seedling r...
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Elsevier
2025-09-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825002936 |
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| author | Miaomiao Huang Yuqing Zhang Wanwei Hou Ping Jin Liang Huang |
| author_facet | Miaomiao Huang Yuqing Zhang Wanwei Hou Ping Jin Liang Huang |
| author_sort | Miaomiao Huang |
| collection | DOAJ |
| description | Stripe rust (Puccinia striiformis f. sp. tritici) poses a major threat to Chinese wheat production. Assessing resistance levels and gene distribution in spring wheat landraces is critical for breeding durable resistant cultivars. This study evaluated 267 landraces from eight provinces for seedling resistance against dominant races CYR32/CYR34 and adult-plant resistance (APR) across five Qinghai natural disease nurseries during 2023–2024. At the seedling stage, 7.5 % (20 accessions; IT 0–6) and 12.4 % (33; IT 0–6) exhibited resistance to CYR32 and CYR34, respectively, with 2.99 % (8 accessions) conferring dual resistance. APR trials identified2.25 % (6 landraces) showed stable resistance, including Banjiemang with complete immunity (IT 0). Molecular analyses revealed prevalent Yr genes, Yr10 (38.6 %,103/267), Yr18 (29.6 %,79/267), Yr26 (20.9 %, 56/267) and multi-gene combinations 60 accessions (22.5 %) with 2 genes; 33 (12.4 %) with ≥3 genes. Notably, 97 accessions (36.3 %) lacked known Yr genes, suggesting novel resistance mechanisms. The Yr18/Yr26 combination emerged as a promising APR source. This study provides germplasm and genetic insights for pyramiding strategies to enhance stripe rust resistance. |
| format | Article |
| id | doaj-art-c1ddf11fdd2f4efeb5eaa4d074a76c73 |
| institution | Kabale University |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
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| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-c1ddf11fdd2f4efeb5eaa4d074a76c732025-08-20T04:02:51ZengElsevierBiochemistry and Biophysics Reports2405-58082025-09-014310220610.1016/j.bbrep.2025.102206Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genesMiaomiao Huang0Yuqing Zhang1Wanwei Hou2Ping Jin3Liang Huang4Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, 810016, China; National Crop Germplasm Resources Duplicate, Xining, Qinghai, 810016, ChinaAcademy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, 810016, China; National Crop Germplasm Resources Duplicate, Xining, Qinghai, 810016, ChinaAcademy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, 810016, China; National Crop Germplasm Resources Duplicate, Xining, Qinghai, 810016, ChinaAcademy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai, 810016, China; Corresponding author.State Key Laboratory for the Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, 100193, China; National Agricultural Experimental Station for Plant Protection, Gangu, Ministry of Agriculture and Rural Affairs, Gansu, 741200, China; Corresponding author. State Key Laboratory for the Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, 100193, China.Stripe rust (Puccinia striiformis f. sp. tritici) poses a major threat to Chinese wheat production. Assessing resistance levels and gene distribution in spring wheat landraces is critical for breeding durable resistant cultivars. This study evaluated 267 landraces from eight provinces for seedling resistance against dominant races CYR32/CYR34 and adult-plant resistance (APR) across five Qinghai natural disease nurseries during 2023–2024. At the seedling stage, 7.5 % (20 accessions; IT 0–6) and 12.4 % (33; IT 0–6) exhibited resistance to CYR32 and CYR34, respectively, with 2.99 % (8 accessions) conferring dual resistance. APR trials identified2.25 % (6 landraces) showed stable resistance, including Banjiemang with complete immunity (IT 0). Molecular analyses revealed prevalent Yr genes, Yr10 (38.6 %,103/267), Yr18 (29.6 %,79/267), Yr26 (20.9 %, 56/267) and multi-gene combinations 60 accessions (22.5 %) with 2 genes; 33 (12.4 %) with ≥3 genes. Notably, 97 accessions (36.3 %) lacked known Yr genes, suggesting novel resistance mechanisms. The Yr18/Yr26 combination emerged as a promising APR source. This study provides germplasm and genetic insights for pyramiding strategies to enhance stripe rust resistance.http://www.sciencedirect.com/science/article/pii/S2405580825002936Spring wheat landracesStripe rustResistance identificationYr |
| spellingShingle | Miaomiao Huang Yuqing Zhang Wanwei Hou Ping Jin Liang Huang Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genes Biochemistry and Biophysics Reports Spring wheat landraces Stripe rust Resistance identification Yr |
| title | Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genes |
| title_full | Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genes |
| title_fullStr | Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genes |
| title_full_unstemmed | Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genes |
| title_short | Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genes |
| title_sort | identification of resistance to stripe rust in 267 chinese spring wheat landraces germplasm and molecular detection of disease resistance genes |
| topic | Spring wheat landraces Stripe rust Resistance identification Yr |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825002936 |
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