Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapy

Hydrogel microspheres hold great promise as scaffolds for bone repair. Their hydrated matrix, biocompatibility, and functional properties make them an attractive choice in regenerative medicine. However, the irregularity of defect requires shape adaptability of the microspheres. Additionally, there...

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Main Authors: Kaixuan Ma, Lei Yang, Wenzhao Li, Kai Chen, Luoran Shang, Yushu Bai, Yuanjin Zhao
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Materials Today Bio
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590006424004241
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author Kaixuan Ma
Lei Yang
Wenzhao Li
Kai Chen
Luoran Shang
Yushu Bai
Yuanjin Zhao
author_facet Kaixuan Ma
Lei Yang
Wenzhao Li
Kai Chen
Luoran Shang
Yushu Bai
Yuanjin Zhao
author_sort Kaixuan Ma
collection DOAJ
description Hydrogel microspheres hold great promise as scaffolds for bone repair. Their hydrated matrix, biocompatibility, and functional properties make them an attractive choice in regenerative medicine. However, the irregularity of defect requires shape adaptability of the microspheres. Additionally, there is still room for improvement regarding the component of the microspheres to achieve sufficient bioactivity. Here, we prepare multi-bioactive microspheres composed of methacrylated silk fibroin (SFMA) via microfluidic electrospray. Magnesium ascorbyl phosphate (MAP) is encapsulated within the microspheres, whose sustained release facilitates angiogenesis and osteogenic differentiation. The microspheres are further coated with a polydopamine (PDA) layer, allowing them to assemble in situ into a scaffold that conforms to the non-uniform contours of bone defects. The photothermal conversion capability of PDA also provides mild photothermal stimulation to further promote bone regeneration. Based on the synergistic effects, our in vivo experiments demonstrated that the microsphere scaffold effectively promotes bone defect healing. Thus, this multi-bioactive scaffold offers a versatile strategy for bone repair with promising clinical potential.
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publishDate 2024-12-01
publisher Elsevier
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series Materials Today Bio
spelling doaj-art-c1d0b651d41e4ec085abca9d772c7dd22024-12-14T06:32:26ZengElsevierMaterials Today Bio2590-00642024-12-0129101363Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapyKaixuan Ma0Lei Yang1Wenzhao Li2Kai Chen3Luoran Shang4Yushu Bai5Yuanjin Zhao6Department of Orthopedics, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, ChinaOujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, ChinaOujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, ChinaDepartment of Orthopedics, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China; Corresponding author.Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, and the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China; Corresponding author.Department of Orthopedics, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China; Corresponding author.Department of Orthopedics, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China; Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; Corresponding author. Department of Orthopedics, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China.Hydrogel microspheres hold great promise as scaffolds for bone repair. Their hydrated matrix, biocompatibility, and functional properties make them an attractive choice in regenerative medicine. However, the irregularity of defect requires shape adaptability of the microspheres. Additionally, there is still room for improvement regarding the component of the microspheres to achieve sufficient bioactivity. Here, we prepare multi-bioactive microspheres composed of methacrylated silk fibroin (SFMA) via microfluidic electrospray. Magnesium ascorbyl phosphate (MAP) is encapsulated within the microspheres, whose sustained release facilitates angiogenesis and osteogenic differentiation. The microspheres are further coated with a polydopamine (PDA) layer, allowing them to assemble in situ into a scaffold that conforms to the non-uniform contours of bone defects. The photothermal conversion capability of PDA also provides mild photothermal stimulation to further promote bone regeneration. Based on the synergistic effects, our in vivo experiments demonstrated that the microsphere scaffold effectively promotes bone defect healing. Thus, this multi-bioactive scaffold offers a versatile strategy for bone repair with promising clinical potential.http://www.sciencedirect.com/science/article/pii/S2590006424004241Bone regenerationHydrogel microsphereAdhesionMicrofluidicsTissue engineering
spellingShingle Kaixuan Ma
Lei Yang
Wenzhao Li
Kai Chen
Luoran Shang
Yushu Bai
Yuanjin Zhao
Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapy
Materials Today Bio
Bone regeneration
Hydrogel microsphere
Adhesion
Microfluidics
Tissue engineering
title Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapy
title_full Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapy
title_fullStr Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapy
title_full_unstemmed Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapy
title_short Mussel-inspired multi-bioactive microsphere scaffolds for bone defect photothermal therapy
title_sort mussel inspired multi bioactive microsphere scaffolds for bone defect photothermal therapy
topic Bone regeneration
Hydrogel microsphere
Adhesion
Microfluidics
Tissue engineering
url http://www.sciencedirect.com/science/article/pii/S2590006424004241
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