Can Scanning Electron Microscopy be used to quantitatively measure dentine roughness?
Objective: The aim of this study was to determine if SEM used with imaging software could be used to quantitatively determine the extent of dentine roughness due to tooth-brushing. Material and methods: Flat, polished dentine surfaces were subjected to 25,000 cycles of simulated tooth-brushing at 2...
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| Format: | Article |
| Language: | English |
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Universidade Estadual Paulista
2024-01-01
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| Series: | Brazilian Dental Science |
| Online Access: | https://ojs.ict.unesp.br/index.php/cob/article/view/4015 |
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| author | Shivaughn Maria Marchan Marsha Ivey Amrita Rajhbeharrysingh Kelee Bascombe |
| author_facet | Shivaughn Maria Marchan Marsha Ivey Amrita Rajhbeharrysingh Kelee Bascombe |
| author_sort | Shivaughn Maria Marchan |
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Objective: The aim of this study was to determine if SEM used with imaging software could be used to quantitatively determine the extent of dentine roughness due to tooth-brushing. Material and methods: Flat, polished dentine surfaces were subjected to 25,000 cycles of simulated tooth-brushing at 2 strokes per second with a load of 200 g. At the end of the brushing cycle, dentine surfaces were first assessed using profilometry and then subjected to SEM analysis. Ra (average roughness) readings were obtained for profilometric assessment. Using imaging software, the horizontal distance between adjacent characteristic grooves noted on micrographs was measured, ensuring that the middle of each crest of the associated groove was used as the reference point, the Crest-to-Crest distance (C-C distance). These two parameters were examined statistically for correlation. Results: When Spearman Rank tests were utilized the correlation between average Ra and crest to crest measurements was 0.709 (p <0.01). The Bland Altman plot, however, showed poor agreement between the two test parameters. Conclusion: Further work is needed to validate the use of this methodology in the quantitative assessment of tooth surface loss due to abrasion.
KEYWORDS
Abrasion; Bland-Altman; Dentine roughness; Profilometry; Scanning electron microscopy.
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| format | Article |
| id | doaj-art-c1a76a368c0f47f5bf7e9638416f5597 |
| institution | Kabale University |
| issn | 2178-6011 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Brazilian Dental Science |
| spelling | doaj-art-c1a76a368c0f47f5bf7e9638416f55972025-08-22T14:49:23ZengUniversidade Estadual PaulistaBrazilian Dental Science2178-60112024-01-0127110.4322/bds.2024.e4015Can Scanning Electron Microscopy be used to quantitatively measure dentine roughness?Shivaughn Maria Marchan0https://orcid.org/0000-0002-1390-8937Marsha Ivey1https://orcid.org/0000-0002-3205-1748Amrita Rajhbeharrysingh2https://orcid.org/0000-0002-3604-6794Kelee Bascombe3https://orcid.org/0000-0002-0119-5554The University of the West Indies, School of Dentistry, Unit of Restorative Dentistry, Mount Hope Medical Complex, Champs Fleurs, Trinidad and TobagoThe University of the West Indies, Public Health and Primary Care, Unit of Paraclinical Sciences, Mount Hope Medical Complex, Champs Fleurs, Trinidad and TobagoThe University of the West Indies, School of Dentistry, Unit of Restorative Dentistry, Mount Hope Medical Complex, Champs Fleurs, Trinidad and TobagoThe University of the West Indies, School of Dentistry, Unit of Child Dental Health, Mount Hope Medical Complex, Champs Fleurs, Trinidad and Tobago Objective: The aim of this study was to determine if SEM used with imaging software could be used to quantitatively determine the extent of dentine roughness due to tooth-brushing. Material and methods: Flat, polished dentine surfaces were subjected to 25,000 cycles of simulated tooth-brushing at 2 strokes per second with a load of 200 g. At the end of the brushing cycle, dentine surfaces were first assessed using profilometry and then subjected to SEM analysis. Ra (average roughness) readings were obtained for profilometric assessment. Using imaging software, the horizontal distance between adjacent characteristic grooves noted on micrographs was measured, ensuring that the middle of each crest of the associated groove was used as the reference point, the Crest-to-Crest distance (C-C distance). These two parameters were examined statistically for correlation. Results: When Spearman Rank tests were utilized the correlation between average Ra and crest to crest measurements was 0.709 (p <0.01). The Bland Altman plot, however, showed poor agreement between the two test parameters. Conclusion: Further work is needed to validate the use of this methodology in the quantitative assessment of tooth surface loss due to abrasion. KEYWORDS Abrasion; Bland-Altman; Dentine roughness; Profilometry; Scanning electron microscopy. https://ojs.ict.unesp.br/index.php/cob/article/view/4015 |
| spellingShingle | Shivaughn Maria Marchan Marsha Ivey Amrita Rajhbeharrysingh Kelee Bascombe Can Scanning Electron Microscopy be used to quantitatively measure dentine roughness? Brazilian Dental Science |
| title | Can Scanning Electron Microscopy be used to quantitatively measure dentine roughness? |
| title_full | Can Scanning Electron Microscopy be used to quantitatively measure dentine roughness? |
| title_fullStr | Can Scanning Electron Microscopy be used to quantitatively measure dentine roughness? |
| title_full_unstemmed | Can Scanning Electron Microscopy be used to quantitatively measure dentine roughness? |
| title_short | Can Scanning Electron Microscopy be used to quantitatively measure dentine roughness? |
| title_sort | can scanning electron microscopy be used to quantitatively measure dentine roughness |
| url | https://ojs.ict.unesp.br/index.php/cob/article/view/4015 |
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