Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma
Background: Oral squamous cell carcinoma (OSCC), being a multifactorial cancer, is significantly higher in South Asian countries and only 70 % of the cases are reported only in its advanced stages. As a component of the renin-angiotensin system (RAS), Angiotensin-Converting Enzyme 2 (ACE2) has been...
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Elsevier
2024-06-01
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| Series: | Oral Oncology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772906024003224 |
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| author | Shivani Sivasakthivel Pratibha Ramani Selvaraj Jayaraman |
| author_facet | Shivani Sivasakthivel Pratibha Ramani Selvaraj Jayaraman |
| author_sort | Shivani Sivasakthivel |
| collection | DOAJ |
| description | Background: Oral squamous cell carcinoma (OSCC), being a multifactorial cancer, is significantly higher in South Asian countries and only 70 % of the cases are reported only in its advanced stages. As a component of the renin-angiotensin system (RAS), Angiotensin-Converting Enzyme 2 (ACE2) has been reported to restrain the progression of hepatocellular carcinoma (HCC) and non-small cell lung cancer (NSCLC) by inhibiting angiogenesis, nevertheless the role of ACE 2 in Oral Squamous Cell Carcinoma progression remains unexplored. Objective: To quantify and compare tissue expression levels of Angiotensin Converting Enzyme 2 in patients with oral squamous cell carcinoma and healthy individuals. Materials and methods: The current study was a case-control study, that includes n = 10 Oral Squamous Cell Carcinoma samples and n = 10, non-pathological tissues taken from healthy subjects, as controls. Fresh tissue samples were collected from the primary tumor areas of OSCC patients and healthy tissue samples from patients who had tooth extraction, and both tissues were stored at −80 °C. RNA isolation was performed using the TRIR kit from Invitrogen. cDNA was synthesized from total RNA isolated from tissues using a first-strand synthesis kit. Finally, ACE2 was assayed using an ultrasensitive human-specific ELISA kit. Statistical analysis was done using independent T test. Results: The data were expressed as mean ± standard deviation and the statistical analysis was done.OSCC patients showed ACE 2 mean optical density of 0.37 with SD ± 0.045 and healthy individuals showed a mean optical density of 0.45 with SD ± 0.052. The mean ng/L scores of OSCC and healthy individuals were 13.57 with SD ± 1.65 and 16.2 with SD ± 1.9 respectively. Optical density and concentration ng/L scores were compared between OSCC samples, showed a minimum value of 0.137 and a maximum of 2.253. Analyzed results shows that the ACE 2 protein expression in OSCC tumour tissue was lower than that in normal tissue (p = 0.004) Conclusion: Angiotensin Converting Enzyme 2 was significantly lower in Oral Squamous Cell Carcinoma tissues when compared with healthy samples. However, further validation in large-scale prospective studies is necessary to explore the role of ACE 2 in prognosis of OSCC. |
| format | Article |
| id | doaj-art-c17ddea47de24e3babfcfc786ad75e15 |
| institution | Kabale University |
| issn | 2772-9060 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Oral Oncology Reports |
| spelling | doaj-art-c17ddea47de24e3babfcfc786ad75e152025-01-09T06:16:27ZengElsevierOral Oncology Reports2772-90602024-06-0110100476Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinomaShivani Sivasakthivel0Pratibha Ramani1Selvaraj Jayaraman2Department of Oral and Maxillofacial Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, India; Corresponding author. Department of Oral and Maxillofacial pathology, Saveetha Dental college and Hospitals, Saveetha institute of Medical and technical sciences, India.Department of Oral and Maxillofacial Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, IndiaCentre of Molecular Medicine and Diagnostics (COMManD), Department of Biochemistry, Saveetha Institute of Medical and Technical Sciences, IndiaBackground: Oral squamous cell carcinoma (OSCC), being a multifactorial cancer, is significantly higher in South Asian countries and only 70 % of the cases are reported only in its advanced stages. As a component of the renin-angiotensin system (RAS), Angiotensin-Converting Enzyme 2 (ACE2) has been reported to restrain the progression of hepatocellular carcinoma (HCC) and non-small cell lung cancer (NSCLC) by inhibiting angiogenesis, nevertheless the role of ACE 2 in Oral Squamous Cell Carcinoma progression remains unexplored. Objective: To quantify and compare tissue expression levels of Angiotensin Converting Enzyme 2 in patients with oral squamous cell carcinoma and healthy individuals. Materials and methods: The current study was a case-control study, that includes n = 10 Oral Squamous Cell Carcinoma samples and n = 10, non-pathological tissues taken from healthy subjects, as controls. Fresh tissue samples were collected from the primary tumor areas of OSCC patients and healthy tissue samples from patients who had tooth extraction, and both tissues were stored at −80 °C. RNA isolation was performed using the TRIR kit from Invitrogen. cDNA was synthesized from total RNA isolated from tissues using a first-strand synthesis kit. Finally, ACE2 was assayed using an ultrasensitive human-specific ELISA kit. Statistical analysis was done using independent T test. Results: The data were expressed as mean ± standard deviation and the statistical analysis was done.OSCC patients showed ACE 2 mean optical density of 0.37 with SD ± 0.045 and healthy individuals showed a mean optical density of 0.45 with SD ± 0.052. The mean ng/L scores of OSCC and healthy individuals were 13.57 with SD ± 1.65 and 16.2 with SD ± 1.9 respectively. Optical density and concentration ng/L scores were compared between OSCC samples, showed a minimum value of 0.137 and a maximum of 2.253. Analyzed results shows that the ACE 2 protein expression in OSCC tumour tissue was lower than that in normal tissue (p = 0.004) Conclusion: Angiotensin Converting Enzyme 2 was significantly lower in Oral Squamous Cell Carcinoma tissues when compared with healthy samples. However, further validation in large-scale prospective studies is necessary to explore the role of ACE 2 in prognosis of OSCC.http://www.sciencedirect.com/science/article/pii/S2772906024003224Angiotensin converting enzyme 2OSCCELISA |
| spellingShingle | Shivani Sivasakthivel Pratibha Ramani Selvaraj Jayaraman Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma Oral Oncology Reports Angiotensin converting enzyme 2 OSCC ELISA |
| title | Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma |
| title_full | Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma |
| title_fullStr | Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma |
| title_full_unstemmed | Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma |
| title_short | Expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma |
| title_sort | expression of angiotensin converting enzyme 2 in patients with oral squamous cell carcinoma |
| topic | Angiotensin converting enzyme 2 OSCC ELISA |
| url | http://www.sciencedirect.com/science/article/pii/S2772906024003224 |
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