Momoridica charantia and fermented Momoridica charantia with Leuconostoc mesenteroides MKSR change intestinal microbial diversity indices and compositions in high-fat and high-cholesterol diet-fed C57BL/6 male mice
IntroductionThis study explores the impact of 4% Momordica charantia (MC) and 4% fermented Momordica charantia (FMC) on gut microbiota and obesity-related health outcomes in male C57BL/6 mice, a model relevant to veterinary sciences for understanding metabolic and gut health disorders in animals.Met...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1496067/full |
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| author | Heewon Moon Kangwook Lee Jung-Heun Ha Na Yeun Kim Hyo Ri Shin Tae Jin Cho Nam Su Oh Jinbong Park Jingsi Tang Jae Kyeom Kim Misook Kim |
| author_facet | Heewon Moon Kangwook Lee Jung-Heun Ha Na Yeun Kim Hyo Ri Shin Tae Jin Cho Nam Su Oh Jinbong Park Jingsi Tang Jae Kyeom Kim Misook Kim |
| author_sort | Heewon Moon |
| collection | DOAJ |
| description | IntroductionThis study explores the impact of 4% Momordica charantia (MC) and 4% fermented Momordica charantia (FMC) on gut microbiota and obesity-related health outcomes in male C57BL/6 mice, a model relevant to veterinary sciences for understanding metabolic and gut health disorders in animals.MethodsMice were assigned to four dietary regimens, including control, high-fat and high-cholesterol diet (POS), POS with 4% MC, and POS with 4% FMC (fermented with Leuconostoc mesenteroides MKSR) over 12 weeks. Fecal samples were collected for 16S rRNA sequencing to evaluate microbial diversity and composition, key factors influencing animal health.ResultsBoth MC and FMC groups exhibited significant alterations in gut microbial communities, with FMC inducing a distinct shift in beta diversity indices. Changes in microbial taxa such as Bacteroidetes, Verrucomicrobia, and Firmicutes were observed, along with enhancement in the ‘L-glutamate and L-glutamine biosynthesis’ pathway. These shifts were associated with reduced body weight gain and liver weights.DiscussionThe findings suggest that MC and FMC have potential benefits for managing diet-induced metabolic disorders and protecting against obesity by modulating gut microbiota and improving gut metabolism. |
| format | Article |
| id | doaj-art-c15976c5a9e147e3887b9a92be2e014d |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-c15976c5a9e147e3887b9a92be2e014d2024-12-17T14:17:43ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.14960671496067Momoridica charantia and fermented Momoridica charantia with Leuconostoc mesenteroides MKSR change intestinal microbial diversity indices and compositions in high-fat and high-cholesterol diet-fed C57BL/6 male miceHeewon Moon0Kangwook Lee1Jung-Heun Ha2Na Yeun Kim3Hyo Ri Shin4Tae Jin Cho5Nam Su Oh6Jinbong Park7Jingsi Tang8Jae Kyeom Kim9Misook Kim10Department of Food Science and Nutrition, Dankook University, Cheonan, Republic of KoreaDepartment of Food Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Food Science and Nutrition, Dankook University, Cheonan, Republic of KoreaDepartment of Food Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Food Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Food Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Food Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Development of Biology, Chinese Academy of Sciences, Beijing, ChinaDepartment of Food Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Food Science and Nutrition, Dankook University, Cheonan, Republic of KoreaIntroductionThis study explores the impact of 4% Momordica charantia (MC) and 4% fermented Momordica charantia (FMC) on gut microbiota and obesity-related health outcomes in male C57BL/6 mice, a model relevant to veterinary sciences for understanding metabolic and gut health disorders in animals.MethodsMice were assigned to four dietary regimens, including control, high-fat and high-cholesterol diet (POS), POS with 4% MC, and POS with 4% FMC (fermented with Leuconostoc mesenteroides MKSR) over 12 weeks. Fecal samples were collected for 16S rRNA sequencing to evaluate microbial diversity and composition, key factors influencing animal health.ResultsBoth MC and FMC groups exhibited significant alterations in gut microbial communities, with FMC inducing a distinct shift in beta diversity indices. Changes in microbial taxa such as Bacteroidetes, Verrucomicrobia, and Firmicutes were observed, along with enhancement in the ‘L-glutamate and L-glutamine biosynthesis’ pathway. These shifts were associated with reduced body weight gain and liver weights.DiscussionThe findings suggest that MC and FMC have potential benefits for managing diet-induced metabolic disorders and protecting against obesity by modulating gut microbiota and improving gut metabolism.https://www.frontiersin.org/articles/10.3389/fvets.2024.1496067/fullMomoridica charantiafermentationLeuconostoc mesenteroides MKSRhigh-fat dietobesitygut microbiota |
| spellingShingle | Heewon Moon Kangwook Lee Jung-Heun Ha Na Yeun Kim Hyo Ri Shin Tae Jin Cho Nam Su Oh Jinbong Park Jingsi Tang Jae Kyeom Kim Misook Kim Momoridica charantia and fermented Momoridica charantia with Leuconostoc mesenteroides MKSR change intestinal microbial diversity indices and compositions in high-fat and high-cholesterol diet-fed C57BL/6 male mice Frontiers in Veterinary Science Momoridica charantia fermentation Leuconostoc mesenteroides MKSR high-fat diet obesity gut microbiota |
| title | Momoridica charantia and fermented Momoridica charantia with Leuconostoc mesenteroides MKSR change intestinal microbial diversity indices and compositions in high-fat and high-cholesterol diet-fed C57BL/6 male mice |
| title_full | Momoridica charantia and fermented Momoridica charantia with Leuconostoc mesenteroides MKSR change intestinal microbial diversity indices and compositions in high-fat and high-cholesterol diet-fed C57BL/6 male mice |
| title_fullStr | Momoridica charantia and fermented Momoridica charantia with Leuconostoc mesenteroides MKSR change intestinal microbial diversity indices and compositions in high-fat and high-cholesterol diet-fed C57BL/6 male mice |
| title_full_unstemmed | Momoridica charantia and fermented Momoridica charantia with Leuconostoc mesenteroides MKSR change intestinal microbial diversity indices and compositions in high-fat and high-cholesterol diet-fed C57BL/6 male mice |
| title_short | Momoridica charantia and fermented Momoridica charantia with Leuconostoc mesenteroides MKSR change intestinal microbial diversity indices and compositions in high-fat and high-cholesterol diet-fed C57BL/6 male mice |
| title_sort | momoridica charantia and fermented momoridica charantia with leuconostoc mesenteroides mksr change intestinal microbial diversity indices and compositions in high fat and high cholesterol diet fed c57bl 6 male mice |
| topic | Momoridica charantia fermentation Leuconostoc mesenteroides MKSR high-fat diet obesity gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1496067/full |
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