Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection
Background. Adenoviruses are highly contagious pathogens which cause respiratory disease particularly in children; they may induce severe disease in infants. Human neutrophil peptides (HNPs) have been found to exhibit antiadenoviral activity. Thus, we have investigated HNPs in nasal aspirates (NAs)...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Canadian Respiratory Journal |
| Online Access: | http://dx.doi.org/10.1155/2018/1038593 |
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| author | V. S. Priyadharshini F. Ramírez-Jiménez M. Molina-Macip C. Renteria-Rosales J. Santiago-Cruz Paola Zarate-Segura E. Lara-Padilla Luis M. Teran |
| author_facet | V. S. Priyadharshini F. Ramírez-Jiménez M. Molina-Macip C. Renteria-Rosales J. Santiago-Cruz Paola Zarate-Segura E. Lara-Padilla Luis M. Teran |
| author_sort | V. S. Priyadharshini |
| collection | DOAJ |
| description | Background. Adenoviruses are highly contagious pathogens which cause respiratory disease particularly in children; they may induce severe disease in infants. Human neutrophil peptides (HNPs) have been found to exhibit antiadenoviral activity. Thus, we have investigated HNPs in nasal aspirates (NAs) of children suffering from adenoviral common cold. Objective. To investigate the release of HNP-1–4 in adenovirus infection and the relationship with self-limiting upper respiratory tract infections. Methods. Nasal aspirate samples (n=14) were obtained from children (aged 6–12 years) infected with adenovirus between June 2012 and December 2015. Control samples were taken 4 weeks after infection when the children were asymptomatic. Levels of HNPs were measured using an enzyme-linked immunosorbent assay (ELISA). Results. There were increased levels of HNP-1, -3, and -4, but not HNP-2, in nasal aspirates (NAs) during adenovirus infections compared to healthy specimens (p≤0.01). Moreover, there was also increase in the neutrophil count, which is a known cell source of HNPs. Conclusion. Our finding supports the involvement of HNP-1, -3, and -4 in naturally occurring cold in children infected with adenovirus. Because of their known antiviral properties, it is tempting to hypothesize that HNPs might play a protective role in adenovirus-induced respiratory disease; however, this remains to be shown. |
| format | Article |
| id | doaj-art-c156787a66b04d4ba5cc6bd4707102d0 |
| institution | Kabale University |
| issn | 1198-2241 1916-7245 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Respiratory Journal |
| spelling | doaj-art-c156787a66b04d4ba5cc6bd4707102d02025-02-03T05:47:22ZengWileyCanadian Respiratory Journal1198-22411916-72452018-01-01201810.1155/2018/10385931038593Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus InfectionV. S. Priyadharshini0F. Ramírez-Jiménez1M. Molina-Macip2C. Renteria-Rosales3J. Santiago-Cruz4Paola Zarate-Segura5E. Lara-Padilla6Luis M. Teran7Escuela Superior de Medicina del Instituto Politecnico Nacional, Plan de San Luis y Díaz Miron, Casco de Santo Tomas, 11340 Mexico City, MexicoInstituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas”, Calzada Tlalpan No. 4502, Sección XVI, 14080 Mexico City, MexicoInstituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas”, Calzada Tlalpan No. 4502, Sección XVI, 14080 Mexico City, MexicoInstituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas”, Calzada Tlalpan No. 4502, Sección XVI, 14080 Mexico City, MexicoInstituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas”, Calzada Tlalpan No. 4502, Sección XVI, 14080 Mexico City, MexicoEscuela Superior de Medicina del Instituto Politecnico Nacional, Plan de San Luis y Díaz Miron, Casco de Santo Tomas, 11340 Mexico City, MexicoComisión de Operación y Fomento de Actividades Académicas del IPN, Mexico City, MexicoInstituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas”, Calzada Tlalpan No. 4502, Sección XVI, 14080 Mexico City, MexicoBackground. Adenoviruses are highly contagious pathogens which cause respiratory disease particularly in children; they may induce severe disease in infants. Human neutrophil peptides (HNPs) have been found to exhibit antiadenoviral activity. Thus, we have investigated HNPs in nasal aspirates (NAs) of children suffering from adenoviral common cold. Objective. To investigate the release of HNP-1–4 in adenovirus infection and the relationship with self-limiting upper respiratory tract infections. Methods. Nasal aspirate samples (n=14) were obtained from children (aged 6–12 years) infected with adenovirus between June 2012 and December 2015. Control samples were taken 4 weeks after infection when the children were asymptomatic. Levels of HNPs were measured using an enzyme-linked immunosorbent assay (ELISA). Results. There were increased levels of HNP-1, -3, and -4, but not HNP-2, in nasal aspirates (NAs) during adenovirus infections compared to healthy specimens (p≤0.01). Moreover, there was also increase in the neutrophil count, which is a known cell source of HNPs. Conclusion. Our finding supports the involvement of HNP-1, -3, and -4 in naturally occurring cold in children infected with adenovirus. Because of their known antiviral properties, it is tempting to hypothesize that HNPs might play a protective role in adenovirus-induced respiratory disease; however, this remains to be shown.http://dx.doi.org/10.1155/2018/1038593 |
| spellingShingle | V. S. Priyadharshini F. Ramírez-Jiménez M. Molina-Macip C. Renteria-Rosales J. Santiago-Cruz Paola Zarate-Segura E. Lara-Padilla Luis M. Teran Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection Canadian Respiratory Journal |
| title | Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection |
| title_full | Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection |
| title_fullStr | Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection |
| title_full_unstemmed | Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection |
| title_short | Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection |
| title_sort | human neutrophil defensin 1 3 and 4 are elevated in nasal aspirates from children with naturally occurring adenovirus infection |
| url | http://dx.doi.org/10.1155/2018/1038593 |
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