ZFP36, an RNA-binding protein promotes hBMSCs osteogenic differentiation via binding with JUN
Abstract Osteoporosis (OP) is a metabolic bone disease characterized by progressive decline of bone mass and bone quality, leading to bone fragility and an increased risk of fracture. The osteogenic differentiation of bone mesenchymal stem cells (BMSCs) is crucial to maintain the balance of osteobla...
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| Format: | Article |
| Language: | English |
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BMC
2024-11-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-024-05232-7 |
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| _version_ | 1846165070287994880 |
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| author | Hairong Su Linyuan Liang Junling Wang Xiaolu Yuan Binxiu Zhao |
| author_facet | Hairong Su Linyuan Liang Junling Wang Xiaolu Yuan Binxiu Zhao |
| author_sort | Hairong Su |
| collection | DOAJ |
| description | Abstract Osteoporosis (OP) is a metabolic bone disease characterized by progressive decline of bone mass and bone quality, leading to bone fragility and an increased risk of fracture. The osteogenic differentiation of bone mesenchymal stem cells (BMSCs) is crucial to maintain the balance of osteoblast and osteoclast. Bioinformatics prediction indicates that ZFP36 ring finger protein (ZFP36), an RNA-binding protein, is a potential target of OP. Herein, we sought to probe the regulatory role and mechanisms of ZFP36 in the progression of OP. Overexpression of ZFP36 enhanced osteoblast viability, differentiation and mineralization of human BMSCs (hBMSCs). RNA immunoprecipitation qPCR (RIP-qPCR) assays demonstrated that ZFP36 could inhibit the translation of JUN, which was also verified with dual luciferase reporter gene assay. Furthermore, administration with T-5224, a transcription factor c-Fos/activator protein (AP)-1 inhibitor, which specifically inhibits the DNA binding activity of c-Fos/JUN, abolished the effect of ZFP36 knockdown on the behaviors of hBMSCs, suggesting that ZFP36 might promotes osteogenic differentiation through regulating JUN. These findings provide insights into the progression and a potential therapeutic target of OP. |
| format | Article |
| id | doaj-art-c10c09c7d2fa4f0c81213226bdc999c2 |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-c10c09c7d2fa4f0c81213226bdc999c22024-11-17T12:38:16ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-11-011911910.1186/s13018-024-05232-7ZFP36, an RNA-binding protein promotes hBMSCs osteogenic differentiation via binding with JUNHairong Su0Linyuan Liang1Junling Wang2Xiaolu Yuan3Binxiu Zhao4Maoming People’s HospitalMaoming People’s HospitalThe Second Affiliated Hospital of Guangzhou, University of Chinese MedicineMaoming People’s HospitalMaoming People’s HospitalAbstract Osteoporosis (OP) is a metabolic bone disease characterized by progressive decline of bone mass and bone quality, leading to bone fragility and an increased risk of fracture. The osteogenic differentiation of bone mesenchymal stem cells (BMSCs) is crucial to maintain the balance of osteoblast and osteoclast. Bioinformatics prediction indicates that ZFP36 ring finger protein (ZFP36), an RNA-binding protein, is a potential target of OP. Herein, we sought to probe the regulatory role and mechanisms of ZFP36 in the progression of OP. Overexpression of ZFP36 enhanced osteoblast viability, differentiation and mineralization of human BMSCs (hBMSCs). RNA immunoprecipitation qPCR (RIP-qPCR) assays demonstrated that ZFP36 could inhibit the translation of JUN, which was also verified with dual luciferase reporter gene assay. Furthermore, administration with T-5224, a transcription factor c-Fos/activator protein (AP)-1 inhibitor, which specifically inhibits the DNA binding activity of c-Fos/JUN, abolished the effect of ZFP36 knockdown on the behaviors of hBMSCs, suggesting that ZFP36 might promotes osteogenic differentiation through regulating JUN. These findings provide insights into the progression and a potential therapeutic target of OP.https://doi.org/10.1186/s13018-024-05232-7OsteoporosisZFP36 ring finger proteinJUNOsteogenic differentiation |
| spellingShingle | Hairong Su Linyuan Liang Junling Wang Xiaolu Yuan Binxiu Zhao ZFP36, an RNA-binding protein promotes hBMSCs osteogenic differentiation via binding with JUN Journal of Orthopaedic Surgery and Research Osteoporosis ZFP36 ring finger protein JUN Osteogenic differentiation |
| title | ZFP36, an RNA-binding protein promotes hBMSCs osteogenic differentiation via binding with JUN |
| title_full | ZFP36, an RNA-binding protein promotes hBMSCs osteogenic differentiation via binding with JUN |
| title_fullStr | ZFP36, an RNA-binding protein promotes hBMSCs osteogenic differentiation via binding with JUN |
| title_full_unstemmed | ZFP36, an RNA-binding protein promotes hBMSCs osteogenic differentiation via binding with JUN |
| title_short | ZFP36, an RNA-binding protein promotes hBMSCs osteogenic differentiation via binding with JUN |
| title_sort | zfp36 an rna binding protein promotes hbmscs osteogenic differentiation via binding with jun |
| topic | Osteoporosis ZFP36 ring finger protein JUN Osteogenic differentiation |
| url | https://doi.org/10.1186/s13018-024-05232-7 |
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