Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)
This study assessed the effect of Bacillus amyloliquefaciens AV5 on the diet of Nile tilapia, focusing on growth, immune responses, and resistance to Streptococcus agalactiae infection. Fingerlings of Oreochromis niloticus were split into three groups and fed diets with different concentrations of B...
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Elsevier
2024-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424004794 |
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| author | Vicent Michael Shija Kwaku Amoah Yi Li Zhong Yong Zhengsi Chen Jia Cai |
| author_facet | Vicent Michael Shija Kwaku Amoah Yi Li Zhong Yong Zhengsi Chen Jia Cai |
| author_sort | Vicent Michael Shija |
| collection | DOAJ |
| description | This study assessed the effect of Bacillus amyloliquefaciens AV5 on the diet of Nile tilapia, focusing on growth, immune responses, and resistance to Streptococcus agalactiae infection. Fingerlings of Oreochromis niloticus were split into three groups and fed diets with different concentrations of B. amyloliquefaciens AV5 for 30 days as follows: no AV5, G1:106 CFU/g, G2:108 CFU/g. Upon completion of the feeding trial, G2 exhibited improved growth performance, including Wf, WGR, and SGR, along with a significantly lower (p< 0.05) feed conversion ratio (FCR) than the other groups. Enhanced immune and biochemical parameters (p < 0.05), including lysozyme (LZM), immunoglobulin M (IgM), alkaline phosphatase (AKP), and acid phosphatase (ACP), as well as antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) and digestive enzymes (trypsin (TYS), lipase (LPS), and protease (PTS)), were observed in group G2 compared to the GC group. No Significant differences in amylase (AML), cellulase (CLS), and xylanase (XYN) levels were observed among the GC, G1, and G2 groups. A substantial decrease (p< 0.05) in GOT, GPT, and MDA activity was observed in G2 compared to that in the GC group. No substantial differences in glucose (GL) levels were observed among the GC, G1, and G2 groups. A substantial increase (p< 0.05) in total protein (TP) was observed in G2 relative to the GC and G1 groups. Significant increases (p< 0.05) were observed in the liver and kidneys of growth- and immunity-related genes in the G2 group, including C-LYZ, GHR-1, TNF-α, IL-1β, and IGF-1. After intraperitoneal injection of S. agalactiae into both control and test fish, the cumulative mortalities in the GC, G1, and G2 groups were 78 %, 51 %, and 14 %, respectively. The findings indicate that administering B. amyloliquefaciens AV5 at a concentration of 1×108cfu/g could potentially yield beneficial outcomes as a probiotic in aquaculture. |
| format | Article |
| id | doaj-art-c0d47a0919d4446ea7fabe386b1731dc |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-c0d47a0919d4446ea7fabe386b1731dc2024-12-03T04:29:05ZengElsevierAquaculture Reports2352-51342024-12-0139102391Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)Vicent Michael Shija0Kwaku Amoah1Yi Li2Zhong Yong3Zhengsi Chen4Jia Cai5College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524002, China; Guangxi Key Laboratory for Marine Natural Products and Combinational Biosynthesis Chemistry, Nanning 530200, China; Guangxi Beibu Gulf Marine Research Centre, Guangxi Academy of Sciences, Nanning 530007, China; Correspondence to: Fisheries College, Guangdong Ocean University, Fisheries College, Guangdong Ocean University, No. 1 of Haida Road, Mazhang District, Zhanjiang, Guangdong Province 524088, China.This study assessed the effect of Bacillus amyloliquefaciens AV5 on the diet of Nile tilapia, focusing on growth, immune responses, and resistance to Streptococcus agalactiae infection. Fingerlings of Oreochromis niloticus were split into three groups and fed diets with different concentrations of B. amyloliquefaciens AV5 for 30 days as follows: no AV5, G1:106 CFU/g, G2:108 CFU/g. Upon completion of the feeding trial, G2 exhibited improved growth performance, including Wf, WGR, and SGR, along with a significantly lower (p< 0.05) feed conversion ratio (FCR) than the other groups. Enhanced immune and biochemical parameters (p < 0.05), including lysozyme (LZM), immunoglobulin M (IgM), alkaline phosphatase (AKP), and acid phosphatase (ACP), as well as antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) and digestive enzymes (trypsin (TYS), lipase (LPS), and protease (PTS)), were observed in group G2 compared to the GC group. No Significant differences in amylase (AML), cellulase (CLS), and xylanase (XYN) levels were observed among the GC, G1, and G2 groups. A substantial decrease (p< 0.05) in GOT, GPT, and MDA activity was observed in G2 compared to that in the GC group. No substantial differences in glucose (GL) levels were observed among the GC, G1, and G2 groups. A substantial increase (p< 0.05) in total protein (TP) was observed in G2 relative to the GC and G1 groups. Significant increases (p< 0.05) were observed in the liver and kidneys of growth- and immunity-related genes in the G2 group, including C-LYZ, GHR-1, TNF-α, IL-1β, and IGF-1. After intraperitoneal injection of S. agalactiae into both control and test fish, the cumulative mortalities in the GC, G1, and G2 groups were 78 %, 51 %, and 14 %, respectively. The findings indicate that administering B. amyloliquefaciens AV5 at a concentration of 1×108cfu/g could potentially yield beneficial outcomes as a probiotic in aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513424004794B. amyloliquefaciensBiochemical and antioxidantGrowth parametersNile tilapiaProbiotics |
| spellingShingle | Vicent Michael Shija Kwaku Amoah Yi Li Zhong Yong Zhengsi Chen Jia Cai Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus) Aquaculture Reports B. amyloliquefaciens Biochemical and antioxidant Growth parameters Nile tilapia Probiotics |
| title | Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus) |
| title_full | Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus) |
| title_fullStr | Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus) |
| title_full_unstemmed | Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus) |
| title_short | Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus) |
| title_sort | influence of dietary bacillus amyloliquefaciens av5 on growth immune responses and resistance to streptococcus agalactiae in nile tilapia oreochromis niloticus |
| topic | B. amyloliquefaciens Biochemical and antioxidant Growth parameters Nile tilapia Probiotics |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424004794 |
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