Flow affects the structural and mechanical properties of the fibrin network in plasma clots
Abstract The fibrin network is one of the main components of thrombi. Altered fibrin network properties are known to influence the development and progression of thrombotic disorders, at least partly through effects on the mechanical stability of fibrin. Most studies investigating the role of fibrin...
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| Format: | Article |
| Language: | English |
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Springer
2024-01-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06775-1 |
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| author | Hande Eyisoylu Emma D. Hazekamp Janneke Cruts Gijsje H. Koenderink Moniek P. M. de Maat |
| author_facet | Hande Eyisoylu Emma D. Hazekamp Janneke Cruts Gijsje H. Koenderink Moniek P. M. de Maat |
| author_sort | Hande Eyisoylu |
| collection | DOAJ |
| description | Abstract The fibrin network is one of the main components of thrombi. Altered fibrin network properties are known to influence the development and progression of thrombotic disorders, at least partly through effects on the mechanical stability of fibrin. Most studies investigating the role of fibrin in thrombus properties prepare clots under static conditions, missing the influence of blood flow which is present in vivo. In this study, plasma clots in the presence and absence of flow were prepared inside a Chandler loop. Recitrated plasma from healthy donors were spun at 0 and 30 RPM. The clot structure was characterized using scanning electron microscopy and confocal microscopy and correlated with the stiffness measured by unconfined compression testing. We quantified fibrin fiber density, pore size, and fiber thickness and bulk stiffness at low and high strain values. Clots formed under flow had thinner fibrin fibers, smaller pores, and a denser fibrin network with higher stiffness values compared to clots formed in absence of flow. Our findings indicate that fluid flow is an essential factor to consider when developing physiologically relevant in vitro thrombus models used in researching thrombectomy outcomes or risk of embolization. Graphical Abstract |
| format | Article |
| id | doaj-art-c0a6b155241d40ec90bb4393b065d88f |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-c0a6b155241d40ec90bb4393b065d88f2024-12-22T12:11:18ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382024-01-0135111010.1007/s10856-024-06775-1Flow affects the structural and mechanical properties of the fibrin network in plasma clotsHande Eyisoylu0Emma D. Hazekamp1Janneke Cruts2Gijsje H. Koenderink3Moniek P. M. de Maat4Department of Hematology, Erasmus MC, University Medical Center RotterdamDepartment of Biomedical Engineering, Thoraxcenter, Erasmus MC, University Medical CenterDepartment of Biomedical Engineering, Thoraxcenter, Erasmus MC, University Medical CenterDepartment of Bionanoscience, Kavli Institute of Nanoscience, Delft University of TechnologyDepartment of Hematology, Erasmus MC, University Medical Center RotterdamAbstract The fibrin network is one of the main components of thrombi. Altered fibrin network properties are known to influence the development and progression of thrombotic disorders, at least partly through effects on the mechanical stability of fibrin. Most studies investigating the role of fibrin in thrombus properties prepare clots under static conditions, missing the influence of blood flow which is present in vivo. In this study, plasma clots in the presence and absence of flow were prepared inside a Chandler loop. Recitrated plasma from healthy donors were spun at 0 and 30 RPM. The clot structure was characterized using scanning electron microscopy and confocal microscopy and correlated with the stiffness measured by unconfined compression testing. We quantified fibrin fiber density, pore size, and fiber thickness and bulk stiffness at low and high strain values. Clots formed under flow had thinner fibrin fibers, smaller pores, and a denser fibrin network with higher stiffness values compared to clots formed in absence of flow. Our findings indicate that fluid flow is an essential factor to consider when developing physiologically relevant in vitro thrombus models used in researching thrombectomy outcomes or risk of embolization. Graphical Abstracthttps://doi.org/10.1007/s10856-024-06775-1 |
| spellingShingle | Hande Eyisoylu Emma D. Hazekamp Janneke Cruts Gijsje H. Koenderink Moniek P. M. de Maat Flow affects the structural and mechanical properties of the fibrin network in plasma clots Journal of Materials Science: Materials in Medicine |
| title | Flow affects the structural and mechanical properties of the fibrin network in plasma clots |
| title_full | Flow affects the structural and mechanical properties of the fibrin network in plasma clots |
| title_fullStr | Flow affects the structural and mechanical properties of the fibrin network in plasma clots |
| title_full_unstemmed | Flow affects the structural and mechanical properties of the fibrin network in plasma clots |
| title_short | Flow affects the structural and mechanical properties of the fibrin network in plasma clots |
| title_sort | flow affects the structural and mechanical properties of the fibrin network in plasma clots |
| url | https://doi.org/10.1007/s10856-024-06775-1 |
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