Effects of Alpinia Officinarum and Euodia Rutaecarpa on the expression of TRPA1 and TRPM8 in rats with irritable bowel syndrome
Objective: Alpinia Officinarum and Euodia Rutaecarpap are two traditional Chinese herbs with warm nature, usually called interior-warming medicinal and widely used to treat cold syndrome for a long history. The purpose of this study is to explore the effects of Alpinia Officinarum and Euodia Rutaeca...
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KeAi Communications Co., Ltd.
2023-03-01
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| Series: | Journal of Holistic Integrative Pharmacy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2707368823000912 |
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| author | Jiahui HE Yuying ZHANG Chaoyan YANG Kunping LI Xujiang YUAN Zihong ZHANG Chiming GU Yanfen CHEN |
| author_facet | Jiahui HE Yuying ZHANG Chaoyan YANG Kunping LI Xujiang YUAN Zihong ZHANG Chiming GU Yanfen CHEN |
| author_sort | Jiahui HE |
| collection | DOAJ |
| description | Objective: Alpinia Officinarum and Euodia Rutaecarpap are two traditional Chinese herbs with warm nature, usually called interior-warming medicinal and widely used to treat cold syndrome for a long history. The purpose of this study is to explore the effects of Alpinia Officinarum and Euodia Rutaecarpap on irritable bowel syndrome (IBS) rats with spleen-stomach deficiency cold syndrome, and to clarify the link between “warming the middle to alleviate pain” and transient receptor potential channel A1 (TRPA1), M8 (TRPM8). Methods: IBS rat model with spleen-stomach deficiency cold syndrome was established by cold diarrhea and restraint stress. Rats were randomly divided into the normal group, model group, positive group (pinaverium bromide), Alpinia Officinarum group and Euodia Rutaecarpa group. After continuous modeling and administration for seven days, body weight, loose stool grades, activity of ATP enzyme, pathological morphology of gastrointestinal tissue were evaluated. The expression of TRPA1 and TRPM8 in spinal ganglion, stomach and colon tissue of rats were detected by RT-PCR and immunohistochemistry. Results: The IBS model with spleen-stomach deficiency cold syndrome caused increase in the number of diarrhea and loss in the body weight in rats. Compared with the normal group, the loose stool grades of model group was significantly increased (P<0.01), Na+-K+-ATP and Ca2+-Mg2+-ATP activity of erythrocyte membrane were significantly decreased (P<0.01). However, compared with the model group, Alpinia Officinarum and Euodia Rutaecarpa obviously reduced the loose stool grades (P<0.05 or P<0.01), and obviously increased the activity of Na+-K+-ATP and Ca2+-Mg2+-ATP (P<0.05). The expression of TRPA1 and TRPM8 in spinal ganglia and colon tissue of the model group was markedly enhanced (P<0.01), but Alpinia Officinarum and Euodia Rutaecarpa significantly inhibited these overexpression. Conclusion: The effect of interior-warming and alleviating pain of Alpinia Officinarum and Euodia Rutaecarpa on IBS model may be mainly achieved by regulating the abnormal expression of TRPA1 and TRPM8. Thus, these results could support the fact that interior-warming medicinal such as Alpinia Officinarum and Euodia Rutaecarpa are traditionally used to cure spleen-stomach deficiency cold syndrome. |
| format | Article |
| id | doaj-art-c0a56fee1ae94d789f6b980692fdc25f |
| institution | Kabale University |
| issn | 2707-3688 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Holistic Integrative Pharmacy |
| spelling | doaj-art-c0a56fee1ae94d789f6b980692fdc25f2024-11-12T05:21:42ZengKeAi Communications Co., Ltd.Journal of Holistic Integrative Pharmacy2707-36882023-03-0141113Effects of Alpinia Officinarum and Euodia Rutaecarpa on the expression of TRPA1 and TRPM8 in rats with irritable bowel syndromeJiahui HE0Yuying ZHANG1Chaoyan YANG2Kunping LI3Xujiang YUAN4Zihong ZHANG5Chiming GU6Yanfen CHEN7School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaInstitute of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaCenter for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaThe second clinical medical college of Guangzhou University of Chinese Medicine, Guangzhou 510120, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China; [Corresponding authors]Objective: Alpinia Officinarum and Euodia Rutaecarpap are two traditional Chinese herbs with warm nature, usually called interior-warming medicinal and widely used to treat cold syndrome for a long history. The purpose of this study is to explore the effects of Alpinia Officinarum and Euodia Rutaecarpap on irritable bowel syndrome (IBS) rats with spleen-stomach deficiency cold syndrome, and to clarify the link between “warming the middle to alleviate pain” and transient receptor potential channel A1 (TRPA1), M8 (TRPM8). Methods: IBS rat model with spleen-stomach deficiency cold syndrome was established by cold diarrhea and restraint stress. Rats were randomly divided into the normal group, model group, positive group (pinaverium bromide), Alpinia Officinarum group and Euodia Rutaecarpa group. After continuous modeling and administration for seven days, body weight, loose stool grades, activity of ATP enzyme, pathological morphology of gastrointestinal tissue were evaluated. The expression of TRPA1 and TRPM8 in spinal ganglion, stomach and colon tissue of rats were detected by RT-PCR and immunohistochemistry. Results: The IBS model with spleen-stomach deficiency cold syndrome caused increase in the number of diarrhea and loss in the body weight in rats. Compared with the normal group, the loose stool grades of model group was significantly increased (P<0.01), Na+-K+-ATP and Ca2+-Mg2+-ATP activity of erythrocyte membrane were significantly decreased (P<0.01). However, compared with the model group, Alpinia Officinarum and Euodia Rutaecarpa obviously reduced the loose stool grades (P<0.05 or P<0.01), and obviously increased the activity of Na+-K+-ATP and Ca2+-Mg2+-ATP (P<0.05). The expression of TRPA1 and TRPM8 in spinal ganglia and colon tissue of the model group was markedly enhanced (P<0.01), but Alpinia Officinarum and Euodia Rutaecarpa significantly inhibited these overexpression. Conclusion: The effect of interior-warming and alleviating pain of Alpinia Officinarum and Euodia Rutaecarpa on IBS model may be mainly achieved by regulating the abnormal expression of TRPA1 and TRPM8. Thus, these results could support the fact that interior-warming medicinal such as Alpinia Officinarum and Euodia Rutaecarpa are traditionally used to cure spleen-stomach deficiency cold syndrome.http://www.sciencedirect.com/science/article/pii/S2707368823000912Alpinia OfficinarumEuodia RutaecarpaInterior-warming medicinalSpleen-stomach cold deficiencyTRPA1TRPM8 |
| spellingShingle | Jiahui HE Yuying ZHANG Chaoyan YANG Kunping LI Xujiang YUAN Zihong ZHANG Chiming GU Yanfen CHEN Effects of Alpinia Officinarum and Euodia Rutaecarpa on the expression of TRPA1 and TRPM8 in rats with irritable bowel syndrome Journal of Holistic Integrative Pharmacy Alpinia Officinarum Euodia Rutaecarpa Interior-warming medicinal Spleen-stomach cold deficiency TRPA1 TRPM8 |
| title | Effects of Alpinia Officinarum and Euodia Rutaecarpa on the expression of TRPA1 and TRPM8 in rats with irritable bowel syndrome |
| title_full | Effects of Alpinia Officinarum and Euodia Rutaecarpa on the expression of TRPA1 and TRPM8 in rats with irritable bowel syndrome |
| title_fullStr | Effects of Alpinia Officinarum and Euodia Rutaecarpa on the expression of TRPA1 and TRPM8 in rats with irritable bowel syndrome |
| title_full_unstemmed | Effects of Alpinia Officinarum and Euodia Rutaecarpa on the expression of TRPA1 and TRPM8 in rats with irritable bowel syndrome |
| title_short | Effects of Alpinia Officinarum and Euodia Rutaecarpa on the expression of TRPA1 and TRPM8 in rats with irritable bowel syndrome |
| title_sort | effects of alpinia officinarum and euodia rutaecarpa on the expression of trpa1 and trpm8 in rats with irritable bowel syndrome |
| topic | Alpinia Officinarum Euodia Rutaecarpa Interior-warming medicinal Spleen-stomach cold deficiency TRPA1 TRPM8 |
| url | http://www.sciencedirect.com/science/article/pii/S2707368823000912 |
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