Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey
<b>Aim:</b> It was aimed to investigate the mecC gene in phenotypically methicillin-resistant Staphylococcus aureus (MRSA), isolated from mastitic milk samples.<p> <b>Materials and Methods:</b> This study was performed on 150 S. aureus strains isolated from mastitic cat...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1131 |
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| author | Zafer Sayın Aslı Sakmanoğlu Uçkun Sait Uçan Yasemin Pınarkara Ali Uslu Zeki Aras Osman Erganiş |
| author_facet | Zafer Sayın Aslı Sakmanoğlu Uçkun Sait Uçan Yasemin Pınarkara Ali Uslu Zeki Aras Osman Erganiş |
| author_sort | Zafer Sayın |
| collection | DOAJ |
| description | <b>Aim:</b> It was aimed to investigate the mecC gene in phenotypically
methicillin-resistant Staphylococcus aureus (MRSA),
isolated from mastitic milk samples.<p>
<b>Materials and Methods:</b> This study was performed on 150
S. aureus strains isolated from mastitic cattle. Resistance to
methicillin was determined by cefoxitin disc diffusion method
and commercial PBP2a latex agglutination test. Detection
of mecA and mecC gene in MRSA isolates were carried out
by Polymerase Chain Reaction (PCR).<p>
<b>Results:</b> Twenty eight (18.6%) out of 150 S. aureus isolates
were identified phenotypically as MRSA by cefoxitin disc
diffusion test. Aglutination and mecA gen were detected in
21 (75%) MRSA isolates by latex agglutination test and PCR,
mecC gene was determined in 7 (25%) MRSA isolates in all of
which no positivity by latex agglutination test and mecA-PCR
analysis was observed.<p>
<b>Conclusion:</b> mecC MRSA isolation rate was determined as to
surprisingly be high in mastitic cattle in Turkey, and mecC
gene should also be further analyzed for genotypic confirmation
of MRSA isolates that already identified by phenotypic
methods. |
| format | Article |
| id | doaj-art-c0a422df988f44c4b8b6db94f80bf231 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-c0a422df988f44c4b8b6db94f80bf2312025-01-03T01:36:16ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19533231821871131Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkeyZafer Sayın0Aslı Sakmanoğlu1Uçkun Sait Uçan2Yasemin Pınarkara3Ali Uslu4Zeki Aras5Osman Erganiş6Selçuk Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Selçuklu, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Selçuklu, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Selçuklu, Konya, TürkiyeSelçuk Üniversitesi, Sarayönü Meslek Yüksek Okulu, Gıda Teknolojisi Bölümü, Sarayönü, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Selçuklu, Konya, TürkiyeAksaray Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Aksaray, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Selçuklu, Konya, Türkiye<b>Aim:</b> It was aimed to investigate the mecC gene in phenotypically methicillin-resistant Staphylococcus aureus (MRSA), isolated from mastitic milk samples.<p> <b>Materials and Methods:</b> This study was performed on 150 S. aureus strains isolated from mastitic cattle. Resistance to methicillin was determined by cefoxitin disc diffusion method and commercial PBP2a latex agglutination test. Detection of mecA and mecC gene in MRSA isolates were carried out by Polymerase Chain Reaction (PCR).<p> <b>Results:</b> Twenty eight (18.6%) out of 150 S. aureus isolates were identified phenotypically as MRSA by cefoxitin disc diffusion test. Aglutination and mecA gen were detected in 21 (75%) MRSA isolates by latex agglutination test and PCR, mecC gene was determined in 7 (25%) MRSA isolates in all of which no positivity by latex agglutination test and mecA-PCR analysis was observed.<p> <b>Conclusion:</b> mecC MRSA isolation rate was determined as to surprisingly be high in mastitic cattle in Turkey, and mecC gene should also be further analyzed for genotypic confirmation of MRSA isolates that already identified by phenotypic methods.http://eurasianjvetsci.org/pdf.php3?id=1131mecameccmastitisstaphylococcus aureusturkey |
| spellingShingle | Zafer Sayın Aslı Sakmanoğlu Uçkun Sait Uçan Yasemin Pınarkara Ali Uslu Zeki Aras Osman Erganiş Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey Eurasian Journal of Veterinary Sciences meca mecc mastitis staphylococcus aureus turkey |
| title | Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey |
| title_full | Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey |
| title_fullStr | Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey |
| title_full_unstemmed | Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey |
| title_short | Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey |
| title_sort | detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey |
| topic | meca mecc mastitis staphylococcus aureus turkey |
| url | http://eurasianjvetsci.org/pdf.php3?id=1131 |
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