Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey
<b>Aim:</b> It was aimed to investigate the mecC gene in phenotypically methicillin-resistant Staphylococcus aureus (MRSA), isolated from mastitic milk samples.<p> <b>Materials and Methods:</b> This study was performed on 150 S. aureus strains isolated from mastitic cat...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1131 |
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| Summary: | <b>Aim:</b> It was aimed to investigate the mecC gene in phenotypically
methicillin-resistant Staphylococcus aureus (MRSA),
isolated from mastitic milk samples.<p>
<b>Materials and Methods:</b> This study was performed on 150
S. aureus strains isolated from mastitic cattle. Resistance to
methicillin was determined by cefoxitin disc diffusion method
and commercial PBP2a latex agglutination test. Detection
of mecA and mecC gene in MRSA isolates were carried out
by Polymerase Chain Reaction (PCR).<p>
<b>Results:</b> Twenty eight (18.6%) out of 150 S. aureus isolates
were identified phenotypically as MRSA by cefoxitin disc
diffusion test. Aglutination and mecA gen were detected in
21 (75%) MRSA isolates by latex agglutination test and PCR,
mecC gene was determined in 7 (25%) MRSA isolates in all of
which no positivity by latex agglutination test and mecA-PCR
analysis was observed.<p>
<b>Conclusion:</b> mecC MRSA isolation rate was determined as to
surprisingly be high in mastitic cattle in Turkey, and mecC
gene should also be further analyzed for genotypic confirmation
of MRSA isolates that already identified by phenotypic
methods. |
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| ISSN: | 1309-6958 2146-1953 |